Characterization of Lectin Resistant Cell Populations Derived from Human Colon Carcinoma: Correlation of K-Ras with β1-6 Branching of N-Linked Carbohydrate and CEA Production

1999 ◽  
Vol 259 (3) ◽  
pp. 588-593 ◽  
Author(s):  
Donald C. Wojciechowicz ◽  
Robert G. Mallon ◽  
Antonio Picon ◽  
Philip B. Paty
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Resistant Cell ◽  
Cell Populations ◽  
Human Colon Carcinoma

scholarly journals Characterization of human colon carcinoma cell lines isolated from a single primary tumour

1983 ◽  
Vol 47 (3) ◽  
pp. 373-381 ◽  
Author(s):  
M G Brattain ◽  
M E Marks ◽  
J McCombs ◽  
W Finely ◽  
D E Brattain
Keyword(s):  
Colon Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Primary Tumour ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Carcinoma Cell Lines ◽  
Human Colon Carcinoma

Ultrasonic characterization of human colon carcinoma cells in the 5-25 MHz frequency range

2017 ◽  
Vol 141 (5) ◽  
pp. 3492-3492
Author(s):  
Amy Longstreth ◽  
Judene Thomas ◽  
Yaa Kwakwa ◽  
Janae Davis ◽  
Maria-Teresa Herd
Keyword(s):  
Colon Carcinoma ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Frequency Range ◽  
Colon Carcinoma Cells ◽  
Ultrasonic Characterization ◽  
Human Colon Carcinoma

Isolation and Characterization of an Undifferentiated Human Colon Carcinoma Cell Line (MIP-101)

1987 ◽  
Vol 5 (6) ◽  
pp. 545-552 ◽  
Author(s):  
Richard M. Niles ◽  
Sally A. Wilhelm ◽  
Glenn D. Steele ◽  
Bohdana Burke ◽  
Thomas Christensen ◽  
...  
Keyword(s):  
Cell Line ◽  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Carcinoma Cell Line ◽  
Human Colon Carcinoma Cell ◽  
Colon Carcinoma Cell Line ◽  
Isolation And Characterization ◽  
Human Colon Carcinoma

scholarly journals Characterization of a family of structurally related glycoproteins expressing β1-6-branched asparagine-linked oligosaccharides in human colon carcinoma cells

1992 ◽  
Vol 283 (1) ◽  
pp. 193-201 ◽  
Author(s):  
S Laferté ◽  
L C Loh
Keyword(s):  
Colon Cancer ◽  
Colon Carcinoma ◽  
Cell Lines ◽  
Culture Medium ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Ht29 Cells ◽  
Human Colon Carcinoma

Previous studies have established that metastatic tumour cells express high levels of beta 1-6-branched Asn-linked oligosaccharides which can be detected with the lectin leucoagglutinin (L-PHA) [Dennis, Laferté, Waghorne, Breitman & Kerbel (1987) Science 236, 582-585]. In order to identify L-PHA-binding glycoproteins which may play a role specifically in colon cancer, we have prepared monoclonal antibodies (MAbs) to the moderately well-differentiated human colon carcinoma cell line HT29. In this paper we present the initial characterization of a family of structurally related L-PHA-binding glycoproteins detected by MAb 1H9 which are differentially expressed and processed by HT29 cells and by two other human colon carcinoma cell lines, SW480 and SW620. In contrast to HT29, the SW480 and SW620 cell lines were established from a poorly differentiated grade III/IV primary tumour and one of its lymph node metastases respectively. MAb 1H9 detects in HT29 cells a conformational determinant present on three L-PHA-binding glycoproteins of 100, 70 and 25kDa, as well as a 74 kDa glycoprotein with high-mannose-type Asn-linked oligosaccharides. Pulse-chase experiments and peptide mapping analyses revealed that the 74 kDa and 100 kDa species are related by carbohydrate processing and are probably derived from a common 76 kDa precursor. On the other hand, the 70 kDa glycoprotein is synthesized from an endoglycosidase H-sensitive precursor of 56 kDa which is structurally related to, but distinct from, the aforementioned 76 kDa precursor. In addition, the 100 kDa species is secreted into the culture medium, whereas the 70 kDa glycoprotein is retained intracellularly. SW480 and SW620 cells showed qualitative and quantitative differences from HT29 cells, including increased secretion of a smaller L-PHA-binding glycoprotein of 92 kDa into the culture medium, as well as apparent differences in glycosylation of the intracellular 66 kDa glycoprotein. These results suggested that the expression, glycosylation and subcellular localization of this family of L-PHA-binding glycoproteins may correlate with the differentiation status of colon cancer cells and/or reflect biochemical changes. characteristic of more progressive metastatic tumours.

Molecular and functional characterization of choline transporter in human colon carcinoma HT-29 cells

2009 ◽  
Vol 483 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Hironobu Kouji ◽  
Masato Inazu ◽  
Tomoko Yamada ◽  
Hirohisa Tajima ◽  
Tatsuya Aoki ◽  
...  
Keyword(s):  
Colon Carcinoma ◽  
Functional Characterization ◽  
Human Colon ◽  
Choline Transporter ◽  
Human Colon Carcinoma ◽  
Ht 29
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