scholarly journals Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum

2008 ◽  
Vol 478 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Kristen E. Whalen ◽  
Dexter Morin ◽  
Ching Yu Lin ◽  
Ronald S. Tjeerdema ◽  
Jared V. Goldstone ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Cdna Cloning ◽  
Marine Gastropod ◽  
Glutathione S Transferases ◽  
Proteomic Identification ◽  
Cyphoma Gibbosum

scholarly journals High-affinity binding of fatty acyl-CoAs and peroxisome proliferator-CoA esters to glutathione S-transferases. Effect on enzymatic activity

1999 ◽  
Vol 266 (1) ◽  
pp. 143-150 ◽  
Author(s):  
Cecilia Silva ◽  
Gloria Loyola ◽  
Rodrigo Valenzuela ◽  
Tea Garcia-Huidobro ◽  
Octavio Monasterio ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Fatty Acyl ◽  
Affinity Binding ◽  
Peroxisome Proliferator ◽  
High Affinity Binding ◽  
High Affinity ◽  
Glutathione S Transferases

A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression

1996 ◽  
Vol 32 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Oshra Blecher ◽  
Noa Erel ◽  
Isabelle Callebaut ◽  
Keren Aviezer ◽  
Adina Breiman
Keyword(s):  
Structural Analysis ◽  
Enzymatic Activity ◽  
Cdna Cloning

scholarly journals Cytochrome P450 diversity and induction by gorgonian allelochemicals in the marine gastropod Cyphoma gibbosum

BMC Ecology ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 24 ◽  
Author(s):  
Kristen E Whalen ◽  
Victoria R Starczak ◽  
David R Nelson ◽  
Jared V Goldstone ◽  
Mark E Hahn
Keyword(s):  
Cytochrome P450 ◽  
Marine Gastropod ◽  
Cyphoma Gibbosum

cDNA cloning, expression, and enzymatic activity of a cellulase from the mulberry longicorn beetle, Apriona germari

2004 ◽  
Vol 139 (1) ◽  
pp. 107-116 ◽  
Author(s):  
Seong Jin Lee ◽  
Seong Ryul Kim ◽  
Hyung Joo Yoon ◽  
Iksoo Kim ◽  
Kwang Sik Lee ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Cdna Cloning ◽  
Longicorn Beetle

cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi

Gene ◽  
2013 ◽  
Vol 514 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Dingguo Xia ◽  
Yadong Wei ◽  
Guozheng Zhang ◽  
Qiaoling Zhao ◽  
Yeshun Zhang ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Cdna Cloning ◽  
Batocera Horsfieldi

scholarly journals Resistance Mechanism to Metsulfuron-Methyl in Polypogon fugax

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1309
Author(s):  
Xiaoyue Yu ◽  
Hanwen Wu ◽  
Jianping Zhang ◽  
Yongjie Yang ◽  
Wei Tang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Sequence Analysis ◽  
Synergistic Effect ◽  
Enzymatic Activity ◽  
Resistance Mechanism ◽  
Acetolactate Synthase ◽  
Metsulfuron Methyl ◽  
Herbicide Metabolism ◽  
Glutathione S Transferases ◽  
Gst Activity

Polypogon fugax is a common winter weed in China and other Asia countries. We have previously found a P. fugax biotype (R) resistant to acetyl co-enzyme A carboxylase (ACCase) herbicides also cannot be effectively controlled by some acetolactate synthase (ALS) herbicides. This study evaluated the level of resistance to four ALS herbicides (metsulfuron-methyl, chlorsulfuron, monosulfuron, pyribambenz isopropyl) in the R biotype and the associated resistance mechanism. The R biotype exhibited moderate level of resistance to metsulfuron-methyl (6.0-fold) compared with the sensitive biotype (S). Sequence analysis of ALS gene revealed that two ALS genes existed in P. fugax. However, no substitution associated with ALS resistance mechanism were found in ALS genes between the S and R biotypes. The activity of ALS enzyme isolated from the R biotype was inherently higher and less sensitive to metsulfuron-methyl than the S biotype. Glutathione S-transferases (GST) activity was also less sensitive to metsulfuron-methyl in the R than as the S biotypes. Malathion, a cytochrome P450 (CYP) monooxygenase inhibitor, had much greater synergistic effect with metsulfuron-methyl on the R than as the S plants, reducing the ED50 value (herbicide dose to inhibit growth by 50%) of metsulfuron-methyl by 23- and 6-fold, respectively, suggesting that CYP mediated enhanced metabolism might contribute to the resistance to ALS herbicides. These results suggest that metsulfuron-methyl resistance in the R biotype was associated with the up-regulated ALS enzymatic activity and the GST and CYP-mediated enhanced herbicide metabolism.

scholarly journals Caloric restriction increases levels of taurine in the intestine and stimulates taurine uptake by conjugation to glutathione

2020 ◽  
Author(s):  
András Gregor ◽  
Marc Pignitter ◽  
Christine Fahrngruber ◽  
Sebastian Bayer ◽  
Veronika Somoza ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Bile Acids ◽  
Caloric Restriction ◽  
Gi Tract ◽  
Taurine Transporter ◽  
Glutathione S Transferases ◽  
Ad Libitum ◽  
Taurine Uptake ◽  
Conjugated Bile Acids

AbstractOur previous study indicated increased levels of taurine-conjugated bile acids in the intestine content of caloric restriction (CR) mice. In the current project, we found increased levels of free taurine and taurine conjugates, including glutathione (GSH)-taurine, in CR compared to ad libitum fed animals in the mucosa along the intestine while there was no impact on taurine and its conjugates in the liver. The levels of free GSH were decreased in the intestine of CR compared to ad libitum fed mice. However, the levels of oxidized GSH were not affected and were complemented by the lack of changes in the antioxidative parameters. Glutathione-S transferases (GST) enzymatic activity was increased as was the expression of GST genes along the GI tract of CR mice. In CR intestine addition of GSH to taurine solution enhanced taurine uptake. Accordingly, the expression of taurine transporter (TauT) was increased in the ileum of CR mice.

scholarly journals cDNA cloning of an alginate lyase from a marine gastropod Aplysia kurodai and assessment of catalytically important residues of this enzyme

Biochimie ◽  
2011 ◽  
Vol 93 (10) ◽  
pp. 1720-1730 ◽  
Author(s):  
Mohammad Matiur Rahman ◽  
Akira Inoue ◽  
Hiroyuki Tanaka ◽  
Takao Ojima
Keyword(s):  
Cdna Cloning ◽  
Alginate Lyase ◽  
Marine Gastropod

Chlorpyrifos-induced hormesis in insecticide-resistant and -susceptible Plutella xylostella under normal and high temperatures

2016 ◽  
Vol 106 (3) ◽  
pp. 378-386 ◽  
Author(s):  
Z.Z. Deng ◽  
F. Zhang ◽  
Z.L. Wu ◽  
Z.Y. Yu ◽  
G. Wu
Keyword(s):  
High Temperature ◽  
Enzymatic Activity ◽  
Plutella Xylostella ◽  
Term Treatment ◽  
Insect Control ◽  
Activity Increase ◽  
Long Term Treatment ◽  
Glutathione S Transferases

AbstractHormesis induced by insecticides at the dosage lower than what ostensibly directly causes death on insects was studied. This paper reports the effects of the in vivo application of varied concentrations of chlorpyrifos (CPF) on Plutella xylostella (DBM). The insecticide concentrations applied included 0.000025–2.5 mg l−1, which are far lower than LC1 (7.2 mg l−1), for the CPF-susceptable (Si) DBM, and 250 mg l−1 which is far below LC1 (1286 mg l−1), for the CPF-resistant (Rc) DBM, as well as LC10- and LC50-doses for both strains. Significant hormesis was found with the ‘hermetic-CPFs’, i.e., 0.0025 mg l−1 for Si DBM and 2.5 mg l−1 for Rc DBM, at the normal or high temperature either in a 24 h or under a long-term treatment. These doses of CPF significantly stimulated the development and increased the fecundity of Si and Rc DBM at 25°C with approximately 23.5–29.8% activity increase on acetylcholinesterase (AChE) and 30.5–91.3% increase on glutathione S-transferases (GSTs) at 25 or 38°C in 4–24 h. The enzymatic activities were significantly reduced by LC50-CPF at 25°C in vivo, but the inhibition was relieved significantly, if the insects were first subjected to a hormetic-CPF pretreatment. It was remarkable that the average rates of enzymatic activity increase were 67.5–76.6% for AChE and 366–546% for GSTs. Consequently, it was concluded that the hormesis on Si and Rc DBM could be induced by CPF doses far below LC1 at normal or high temperature in short- or long-term treatment. These findings might help to improve the current insect control practices in the field.

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