A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression

1996 ◽  
Vol 32 (3) ◽  
pp. 493-504 ◽  
Author(s):  
Oshra Blecher ◽  
Noa Erel ◽  
Isabelle Callebaut ◽  
Keren Aviezer ◽  
Adina Breiman
Keyword(s):  
Structural Analysis ◽  
Enzymatic Activity ◽  
Cdna Cloning

cDNA cloning and structural analysis of the human limbic-system-associated membrane protein (LAMP)

Gene ◽  
1996 ◽  
Vol 170 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Aurea F. Pimenta ◽  
Itzhak Fischer ◽  
Pat Levitt
Keyword(s):  
Structural Analysis ◽  
Membrane Protein ◽  
Cdna Cloning ◽  
Limbic System

Enzymatic activity with an incomplete catalytic spine: insights from a comparative structural analysis of human CK2α and its paralogous isoform CK2α′

2011 ◽  
Vol 356 (1-2) ◽  
pp. 57-65 ◽  
Author(s):  
Nils Bischoff ◽  
Jennifer Raaf ◽  
Birgitte Olsen ◽  
Maria Bretner ◽  
Olaf-Georg Issinger ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Enzymatic Activity

cDNA cloning, structural analysis, SNP detection and tissue expression profile of the IGF1 gene in Malabari and Attappady Black goats of India

2017 ◽  
Vol 96 (2) ◽  
pp. 307-312 ◽  
Author(s):  
THOMAS NAICY ◽  
THIRUPATHY VENKATACHALAPATHY ◽  
THAZHATHUVEETTIL ARAVINDAKSHAN ◽  
KUNNIYOOR CHEEMANI RAGHAVAN ◽  
MANGATTUMURUPPEL MINI ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Cdna Cloning ◽  
Expression Profile ◽  
Tissue Expression ◽  
Snp Detection ◽  
Tissue Expression Profile

scholarly journals Structural Analysis of Mammalian Cytochrome P450 2B4 Covalently Bound to the Mechanism-Based Inactivatortert-Butylphenylacetylene: Insight into Partial Enzymatic Activity

Biochemistry ◽  
2011 ◽  
Vol 50 (22) ◽  
pp. 4903-4911 ◽  
Author(s):  
Sean C. Gay ◽  
Haoming Zhang ◽  
P. Ross Wilderman ◽  
Arthur G. Roberts ◽  
Tong Liu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Structural Analysis ◽  
Enzymatic Activity ◽  
Cytochrome P450 2B4 ◽  
Insight Into ◽  
Covalently Bound

scholarly journals Structural Evidence of Functional Divergence in Human Alkaline Phosphatases

2002 ◽  
Vol 277 (51) ◽  
pp. 49808-49814 ◽  
Author(s):  
Marie-Hélène Le Du ◽  
José Luis Millán
Keyword(s):  
Alkaline Phosphatase ◽  
Structural Analysis ◽  
Enzymatic Activity ◽  
Germ Cell ◽  
Active Site ◽  
Functional Divergence ◽  
Alkaline Phosphatases ◽  
Tissue Specific ◽  
Active Site Cleft ◽  
Structural Differences

The evolution of the alkaline phosphatase (AP) gene family has lead to the existence in humans of one tissue-nonspecific (TNAP) and three tissue-specific isozymes,i.e.intestinal (IAP), germ cell (GCAP), and placental AP (PLAP). To define the structural differences between these isozymes, we have built models of the TNAP, IAP, and GCAP molecules based on the 1.8-Å structure of PLAP (1) and have performed a comparative structural analysis. We have examined the monomer-monomer interface as this area is crucial for protein stability and enzymatic activity. We found that the interface allows the formation of heterodimers among IAP, GCAP, and PLAP but not between TNAP with any of the three tissue-specific isozymes. Secondly, the active site cleft was mapped into three regions,i.e.the active site itself, the roof of the cleft, and the floor of the cleft. This analysis led to a structural fingerprint of the active site of each AP isozyme that suggests a diversification in substrate specificity for this isozyme family.

Osmia cornifronsvitellogenin: cDNA cloning, structural analysis and developmental expression

2015 ◽  
Vol 45 (2) ◽  
pp. 94-101 ◽  
Author(s):  
Kyeong Yong Lee ◽  
Hyung Joo Yoon ◽  
Byung Rae Jin
Keyword(s):  
Structural Analysis ◽  
Cdna Cloning ◽  
Developmental Expression

cDNA cloning, expression, and enzymatic activity of a cellulase from the mulberry longicorn beetle, Apriona germari

2004 ◽  
Vol 139 (1) ◽  
pp. 107-116 ◽  
Author(s):  
Seong Jin Lee ◽  
Seong Ryul Kim ◽  
Hyung Joo Yoon ◽  
Iksoo Kim ◽  
Kwang Sik Lee ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Cdna Cloning ◽  
Longicorn Beetle

cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi

Gene ◽  
2013 ◽  
Vol 514 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Dingguo Xia ◽  
Yadong Wei ◽  
Guozheng Zhang ◽  
Qiaoling Zhao ◽  
Yeshun Zhang ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Cdna Cloning ◽  
Batocera Horsfieldi
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