A GATA-type transcription factor regulates expression of the high-affinity iron uptake system in the methylotrophic yeast Pichia pastoris

2007 ◽  
Vol 465 (1) ◽  
pp. 172-179 ◽  
Author(s):  
Rossella Miele ◽  
Donatella Barra ◽  
Maria Carmela Bonaccorsi di Patti
Keyword(s):  
Transcription Factor ◽  
Pichia Pastoris ◽  
Iron Uptake ◽  
Methylotrophic Yeast ◽  
Uptake System ◽  
Yeast Pichia Pastoris ◽  
High Affinity ◽  
Iron Uptake System ◽  
Methylotrophic Yeast Pichia Pastoris

Cloning of Pichia pastoris Fet3: Insights into the High Affinity Iron Uptake System

2001 ◽  
Vol 392 (1) ◽  
pp. 162-167 ◽  
Author(s):  
Maria Paola Paronetto ◽  
Rossella Miele ◽  
Antonella Maugliani ◽  
Marina Borro ◽  
Maria Carmela Bonaccorsi di Patti
Keyword(s):  
Pichia Pastoris ◽  
Iron Uptake ◽  
Uptake System ◽  
High Affinity ◽  
Iron Uptake System

The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris

2014 ◽  
Vol 1841 (2) ◽  
pp. 215-226 ◽  
Author(s):  
Lisa Klug ◽  
Pablo Tarazona ◽  
Clemens Gruber ◽  
Karlheinz Grillitsch ◽  
Brigitte Gasser ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Methylotrophic Yeast ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris

scholarly journals Positive Selection of Novel Peroxisome Biogenesis-Defective Mutants of the Yeast Pichia pastoris

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1379-1391
Author(s):  
Monique A Johnson ◽  
Hans R Waterham ◽  
Galyna P Ksheminska ◽  
Liubov R Fayura ◽  
Joan Lin Cereghino ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Allyl Alcohol ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeast ◽  
Direct Selection ◽  
Toxic Exposure ◽  
Peroxisome Biogenesis ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Selection Of

Abstract We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.

scholarly journals Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris

2013 ◽  
Vol 1831 (2) ◽  
pp. 282-290 ◽  
Author(s):  
Vasyl A. Ivashov ◽  
Karlheinz Grillitsch ◽  
Harald Koefeler ◽  
Erich Leitner ◽  
Dominic Baeumlisberger ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Lipid Droplets ◽  
Methylotrophic Yeast ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris

scholarly journals Yeast protective response to arsenate involves the repression of the high affinity iron uptake system

2013 ◽  
Vol 1833 (5) ◽  
pp. 997-1005 ◽  
Author(s):  
Liliana Batista-Nascimento ◽  
Michel B. Toledano ◽  
Dennis J. Thiele ◽  
Claudina Rodrigues-Pousada
Keyword(s):  
Iron Uptake ◽  
Uptake System ◽  
Protective Response ◽  
High Affinity ◽  
Iron Uptake System
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