Proteoglycan-substrate gel zymography for the detection of chondroitin sulfate-degrading enzymes

2019 ◽  
Vol 568 ◽  
pp. 51-52
Author(s):  
Takashi Kobayashi ◽  
Ikuko Kakizaki ◽  
Toshiya Nakamura
Keyword(s):  
Chondroitin Sulfate ◽  
Degrading Enzymes

scholarly journals Chondroitin Sulfate-Degrading Enzymes as Tools for the Development of New Pharmaceuticals

Catalysts ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 322 ◽  
Author(s):  
Raúl Benito-Arenas ◽  
Sandra Zárate ◽  
Julia Revuelta ◽  
Agatha Bastida
Keyword(s):  
Extracellular Matrix ◽  
Pharmaceutical Industry ◽  
Chondroitin Sulfate ◽  
Biological Tissues ◽  
Sulfated Polysaccharides ◽  
Specific Time ◽  
Time Points ◽  
Therapeutic Applications ◽  
Degrading Enzymes ◽  
Chondroitin Sulfates

Chondroitin sulfates are linear anionic sulfated polysaccharides found in biological tissues, mainly within the extracellular matrix, which are degraded and altered by specific lyases depending on specific time points. These polysaccharides have recently acquired relevance in the pharmaceutical industry due to their interesting therapeutic applications. As a consequence, chondroitin sulfate (CS) lyases have been widely investigated as tools for the development of new pharmaceuticals based on these polysaccharides. This review focuses on the major breakthrough represented by chondroitin sulfate-degrading enzymes and their structures and mechanisms of function in addition to their major applications.

scholarly journals Research and Application of Chondroitin Sulfate/Dermatan Sulfate-Degrading Enzymes

2020 ◽  
Vol 8 ◽  
Author(s):  
Wenshuang Wang ◽  
Liran Shi ◽  
Yong Qin ◽  
Fuchuan Li
Keyword(s):  
Chondroitin Sulfate ◽  
Dermatan Sulfate ◽  
Structural Complexity ◽  
Medical Application ◽  
Composition Analysis ◽  
Biological Processes ◽  
Functional Studies ◽  
Degrading Enzymes ◽  
Site Of Action ◽  
Basic Studies

Chondroitin sulfate (CS) and dermatan sulfate (DS) are widely distributed on the cell surface and in the extracellular matrix in the form of proteoglycan, where they participate in various biological processes. The diverse functions of CS/DS can be mainly attributed to their high structural variability. However, their structural complexity creates a big challenge for structural and functional studies of CS/DS. CS/DS-degrading enzymes with different specific activities are irreplaceable tools that could be used to solve this problem. Depending on the site of action, CS/DS-degrading enzymes can be classified as glycosidic bond-cleaving enzymes and sulfatases from animals and microorganisms. As discussed in this review, a few of the identified enzymes, particularly those from bacteria, have wildly applied to the basic studies and applications of CS/DS, such as disaccharide composition analysis, the preparation of bioactive oligosaccharides, oligosaccharide sequencing, and potential medical application, but these do not fulfill all of the needs in terms of the structural complexity of CS/DS.

Effect of Glycosaminoglycans on Thrombin- and Atroxin-Induced Fibrin Assembly and Structure

1989 ◽  
Vol 62 (04) ◽  
pp. 1057-1061 ◽  
Author(s):  
Marcus E Carr ◽  
Patrick L Powers
Keyword(s):  
Chondroitin Sulfate ◽  
Fiber Diameter ◽  
Lag Phase ◽  
Low Molecular Weight ◽  
Fibrin Gels ◽  
Fiber Size ◽  
Induced Changes ◽  
The Impact ◽  
Fiber Radius ◽  
Turbidity Increase

SummaryThis study was performed to quantitate the impact of several glycosaminoglycans (GAG) on fibrin assembly and structure. Gel formation was monitored as the increase in optical density at 633 nm subsequent to thrombin (2 NIH u/ml) or atroxin (0.10 mg/ml) addition to solutions of buffered fibrinogen (1 mg/ml) or plasma. Gel absorbance was measured as a function of wavelength (400 to 800 nm) and gel fiber diameter and mass/length ratio (μ) were calculated. Chondroitin sulfate A (CSA)shortened the lag phase, enhanced the maximal rate of turbidity increase, and increased the final gel turbidity of fibrin gels formed by thrombin or atroxin. CSA (16 mg/ml) increased fiber μ from 1.3 to 3.1 × 1013 dalton/cm and fiber radius from 6.0 to 8.6 × 10-6 cm in thrombin-induced gels. μ increased from 0.7 to 2.7 × 1013 dalton/cm and fiber radius from 4 to 7.8 × 10-6 cm for atroxin-induced gels. Above 16 mg/ml, CSA caused fibrinogen precipitation in purified solutions but not in plasma. CSA inhibited thrombin-induced plasma clotting of plasma but effects in atroxin-mediated plasma gels paralleled those seen in purified solutions. Chondroitin sulfate B (CSB)-induced changes in fibrin were similar but slightly less dramatic than those seen with CSA. μ increased from 0.9 to 2.0 × 1013 dalton/cm for thrombin-induced fibrin gels and from 0.8 to 2.3 × 1013 dalton/cm for atroxininduced gels. Low molecular weight heparin (Mr = 5100) slowed fibrin assembly and reduced fiber size by 50% in thrombininduced gels. Changes in μ of atroxin-induced gels were much less pronounced (<20%). This study documents pronounced GAGinduced changes in fibrin structure which vary with GAG species and may mediate significant physiologic functions.

The Gut Microbiota of Rats under Experimental Osteoarthritis and Administration of Chondroitin Sulfate and Probiotic

2020 ◽  
Vol 82 (6) ◽  
pp. 64-73
Author(s):  
O.H. Korotkyi ◽  
◽  
T.V. Luhovska ◽  
T.M. Serhiychuk ◽  
K.O. Dvorshchenko ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Chondroitin Sulfate ◽  
Joint Inflammation ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Quantitative Composition ◽  
Combined Administration ◽  
Osteoarthritis Progression ◽  
Experimental Osteoarthritis ◽  
Gut Microbiota Composition

Osteoarthritis is a most widespread chronic degenerative joint disease that causes pain, cartilage deformation, and joint inflammation. Adverse alterations of intestinal microbiota like dysbiosis may lead to metabolic syndrome and inflammation, two important components of osteoarthritis progression. Aim. In this study we investigated the effect of chondroitin sulfate and probiotics on the gut microbiome in monoiodoacetate-induced osteoarthritis model in rats. Methods. The species and quantitative composition of feces were determined using diagnostic media with selective properties. Further identification of isolated microorganisms was carried out according to morphological, tinctorial, physiological and metabolic parameters. The results are presented in the form of lg CFU/g. Results. Induction of osteoarthritis caused significant increasing the number of opportunistic enterobacteria and lactose-negative Escherichia coli against the decreasing of lacto- and bifidobacteria that may indicate a dysbiotic condition. Coadministration of chondroitin sulfate and probiotic bacteria has led to improvement the quantitative composition of the gut microbiota in experimental animals, the numerous of Bifidobacterium, Lactobacillus were increasing against decreasing the quantitative composition of opportunistic microorganisms. Conclusions. Monoiodoacetate-induced osteoarthritis caused dysbiosis of gut in rat. We observed beneficial effect of combined administration of chondroitin sulfate and probiotics on gut microbiota composition in rats with experimental osteoarthritis. Thus, adding of supplements like probiotics to standard treatment of osteoarthritis may have potentials to prevent and treat this disease.

Effect of Olive Oil Mill Waste Waters on the Edible and Medicinal Mushroom Lentinus edodes (Berk.) Sing. Growth and Lignin Degrading Enzymes

2002 ◽  
Vol 4 (2) ◽  
pp. 10 ◽  
Author(s):  
Slaven Zjalic ◽  
Anna Adele Fabbri ◽  
Alessandra Ricelli ◽  
Massimo Reverberi ◽  
Emanuela Galli ◽  
...  
Keyword(s):  
Olive Oil ◽  
Medicinal Mushroom ◽  
Lentinus Edodes ◽  
Waste Waters ◽  
Degrading Enzymes ◽  
Mill Waste

Genetically Reduced Chondroitin Sulfate Prevents the Progression of Diabetic Neuropathy

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 547-P
Author(s):  
HAJIME ISHIGURO ◽  
TAKASHI USHIKI ◽  
ASAMI KAWASAKI ◽  
KAORI CHO ◽  
MASAYOSHI MASUKO ◽  
...  
Keyword(s):  
Diabetic Neuropathy ◽  
Chondroitin Sulfate
Sign in / Sign up

Export Citation Format

Share Document