A carbon nanotube-enhanced real-time immuno-PCR for ultrasensitive detection of AHTN in water

2018 ◽  
Vol 544 ◽  
pp. 22-28 ◽  
Author(s):  
Xiaohan Zhang ◽  
Huisheng Zhuang
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Ultrasensitive Detection

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

scholarly journals Real-Time Monitoring of Cellular Cultures with Electrolyte-Gated Carbon Nanotube Transistors

2019 ◽  
Vol 11 (41) ◽  
pp. 37966-37972 ◽  
Author(s):  
Francesca Scuratti ◽  
Giorgio E. Bonacchini ◽  
Caterina Bossio ◽  
Jorge M. Salazar-Rios ◽  
Wytse Talsma ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Real Time Monitoring ◽  
Carbon Nanotube Transistors ◽  
Nanotube Transistors

scholarly journals Performance Evaluation of a Carbon Nanotube Sensor for Fatigue Crack Monitoring of Metal Structures

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4383
Author(s):  
Shafique Ahmed ◽  
Thomas Schumacher ◽  
Erik T. Thostenson ◽  
Jennifer McConnell
Keyword(s):  
Fatigue Crack ◽  
Carbon Nanotube ◽  
Crack Initiation ◽  
Crack Propagation ◽  
Real Time ◽  
False Positive ◽  
Fatigue Cracks ◽  
Fatigue Crack Initiation ◽  
Resistance Change ◽  
Metal Structures

This article describes research that investigated the ability of a carbon nanotube (CNT) sensor to detect and monitor fatigue crack initiation and propagation in metal structures. The sensor consists of a nonwoven carrier fabric with a thin film of CNT that is bonded to the surface of a structure using an epoxy adhesive. The carrier fabric enables the sensor to be easily applied over large areas with complex geometries. Furthermore, the distributed nature of the sensor improves the probability of detecting crack initiation and enables monitoring of crack propagation over time. Piezoresistivity of the sensor enables strains to be monitored in real time and the sensor, which is designed to fragment as fatigue cracks propagate, directly measures crack growth through permanent changes in resistance. The following laboratory tests were conducted to evaluate the performance of the sensor: (1) continuous crack propagation monitoring, (2) potential false positive evaluation under near-threshold crack propagation conditions, and (3) crack re-initiation detection at a crack-stop hole, which is a commonly used technique to arrest fatigue cracks. Real-time sensor measurements and post-mortem fractography show that a distinguishable resistance change of the sensor occurs due to fatigue crack propagation that can be quantitatively related to crack length. The sensor does not show false positive responses when the crack does not propagate, which is a drawback of many other fatigue sensors. The sensor is also shown to be remarkably sensitive to detecting crack re-initiation.

Sign in / Sign up

Export Citation Format

Share Document