Exploration of AFM Imaging Artifacts Occurring at Sharp Surface Features When Using Short Carbon Nanotube Probes and Possible Mitigation With Real-Time Force Spectroscopy

2010 ◽  
Vol 132 (3) ◽  
pp. 030904 ◽  
Author(s):  
Santiago D. Solares ◽  
Gaurav Chawla
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Force Spectroscopy ◽  
Surface Features ◽  
Afm Imaging ◽  
Imaging Artifacts ◽  
Short Carbon

Enhanced Topographical Characterization of Sharp Step Edges With Simultaneous AFM Imaging and Force Spectroscopy

2010 ◽  
Author(s):  
Santiago D. Solares
Keyword(s):  
Force Spectroscopy ◽  
Modulation Method ◽  
Inversion Method ◽  
Dual Frequency ◽  
Force Microscopy ◽  
Atomic Force ◽  
Experimental Implementation ◽  
Afm Imaging ◽  
Imaging Artifacts ◽  
Step Edges

Topographical imaging with atomic force microscopy (AFM) has become an established method since its invention in 1986. However, there exist a variety of imaging artifacts that can distort the acquired images, especially when using sharp probes such as carbon nanotubes or nanowires. This paper briefly discusses common imaging artifacts occurring at sharp step edges and explores theoretically their mitigation with spectral and multi-frequency methods that can perform simultaneous topographical imaging and force spectroscopy. The work focuses on the spectral inversion method, which has been experimentally validated by others [Stark et al., Proc. Natl. Acad. Sci. USA 99, 8473–8478 (2002); Sahin et al., Nature Nanotech. 2, 507–514 (2007)], and on a recently proposed dual-frequency-modulation method, which has been demonstrated within computational simulations and is under experimental implementation in our laboratory [Solares & Chawla, Meas. Sci. & Technol. 19, No. 055502; Chawla & Solares, Meas. Sci. & Technol. 20, No. 015501].

Voltammetric Detection of Tetrodotoxin Real-Time In Vivo of Mouse Organs using DNA-Immobilized Carbon Nanotube Sensors

2019 ◽  
Vol 15 (5) ◽  
pp. 567-574
Author(s):  
Huck Jun Hong ◽  
Suw Young Ly
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Anticancer Agents ◽  
Cancer Metastasis ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Takifugu Rubripes ◽  
In Vivo Detection ◽  
Voltammetric Detection

Background: Tetrodotoxin (TTX) is a biosynthesized neurotoxin that exhibits powerful anticancer and analgesic abilities by inhibiting voltage-gated sodium channels that are crucial for cancer metastasis and pain delivery. However, for the toxin’s future medical applications to come true, accurate, inexpensive, and real-time in vivo detection of TTX remains as a fundamental step. Methods: In this study, highly purified TTX extracted from organs of Takifugu rubripes was injected and detected in vivo of mouse organs (liver, heart, and intestines) using Cyclic Voltammetry (CV) and Square Wave Anodic Stripping Voltammetry (SWASV) for the first time. In vivo detection of TTX was performed with auxiliary, reference, and working herring sperm DNA-immobilized carbon nanotube sensor systems. Results: DNA-immobilization and optimization of amplitude (V), stripping time (sec), increment (mV), and frequency (Hz) parameters for utilized sensors amplified detected peak currents, while highly sensitive in vivo detection limits, 3.43 µg L-1 for CV and 1.21 µg L-1 for SWASV, were attained. Developed sensors herein were confirmed to be more sensitive and selective than conventional graphite rodelectrodes modified likewise. A linear relationship was observed between injected TTX concentration and anodic spike peak height. Microscopic examination displayed coagulation and abnormalities in mouse organs, confirming the powerful neurotoxicity of extracted TTX. Conclusion: These results established the diagnostic measures for TTX detection regarding in vivo application of neurotoxin-deviated anticancer agents and analgesics, as well as TTX from food poisoning and environmental contamination.

Kinetics of interaction of HIV fusion protein (gp41) with lipid membranes studied by real-time AFM imaging

2010 ◽  
Vol 110 (6) ◽  
pp. 694-700 ◽  
Author(s):  
Arkady Bitler ◽  
Naama Lev ◽  
Yael Fridmann-Sirkis ◽  
Lior Blank ◽  
Sidney R. Cohen ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Real Time ◽  
Lipid Membranes ◽  
Afm Imaging ◽  
Protein Gp41 ◽  
Kinetics Of

scholarly journals Real-Time Monitoring of Cellular Cultures with Electrolyte-Gated Carbon Nanotube Transistors

2019 ◽  
Vol 11 (41) ◽  
pp. 37966-37972 ◽  
Author(s):  
Francesca Scuratti ◽  
Giorgio E. Bonacchini ◽  
Caterina Bossio ◽  
Jorge M. Salazar-Rios ◽  
Wytse Talsma ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
Real Time Monitoring ◽  
Carbon Nanotube Transistors ◽  
Nanotube Transistors
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