scholarly journals Enzymatic amplification of DNA/RNA hybrid molecular beacon signaling in nucleic acid detection

2013 ◽  
Vol 432 (2) ◽  
pp. 106-114 ◽  
Author(s):  
Thomas Jacroux ◽  
Daniel C. Rieck ◽  
Rong Cui ◽  
Yexin Ouyang ◽  
Wen-Ji Dong
Keyword(s):  
Nucleic Acid ◽  
Molecular Beacon ◽  
Nucleic Acid Detection ◽  
Enzymatic Amplification

Assembled molecular beacon-based self-propelled DNA machine for enzyme-free and distinctly amplified nucleic acid detection

2021 ◽  
Vol 339 ◽  
pp. 129877
Author(s):  
Jialong Wang ◽  
Shunjun Xie ◽  
Dengren Liu ◽  
Hong Zhou ◽  
Li Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Beacon ◽  
Nucleic Acid Detection ◽  
Dna Machine

scholarly journals A molecular beacon-like Ag nanoclusters fluorescence probe for nucleic acid detection

2021 ◽  
Author(s):  
Ying ZHAO ◽  
Hao ZHANG ◽  
Lili LIAN ◽  
Xiyue WANG ◽  
Wenxiu GAO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Beacon ◽  
Fluorescence Probe ◽  
Nucleic Acid Detection ◽  
Ag Nanoclusters

scholarly journals A Self-Immolative Molecular Beacon for Amplified Nucleic Acid Detection

2021 ◽  
Author(s):  
Magdalena Roth ◽  
Oliver Seitz
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Molecular Beacon ◽  
Homogeneous Solution ◽  
Nucleic Acid Detection ◽  
Loop Region ◽  
Hybridization Probes ◽  
The One ◽  
Reporter Group ◽  
Rna And Dna

Fluorogenic hybridization probes allow the detection of RNA and DNA sequences in homogeneous solution. Typically, one target molecule is activating the fluorescence of a single probe molecule. This limits the sensitivity of nucleic acid detection. Herein, we report a self-immolative Molecular Beacon (iMB), which escapes the one-target-one-probe dogma. The iMB probe includes a photoreductively cleavable N-alkylpicolinium (NAP) linkage within the loop region. A fluorophore at the 5'-end serves, on the one hand, as a reporter group and, on the other hand, as a photosensitizer of a NAP-linker cleavage reaction. In the absence of a target, the iMB adopts a hairpin shape. Quencher proups prevent photo-induced cleavage. The iMB opens upon hybridization with target, and both fluorescent emission as well as photo-inductive cleavage of the NAP-linker can occur. In contrast to previous chemical amplification probes, iMBs are unimolecular. Cleavage leads to products that have lower target affinity than the probes before reaction. Aided by catalysis, the method allowed the detection of 5 pM RNA target within 100 min. <br>

A Self-Immolative Molecular Beacon for Amplified Nucleic Acid Detection

2021 ◽  
Author(s):  
Magdalena Roth ◽  
Oliver Seitz
Keyword(s):  
Nucleic Acid ◽  
Dna Sequences ◽  
Molecular Beacon ◽  
Homogeneous Solution ◽  
Nucleic Acid Detection ◽  
Loop Region ◽  
Hybridization Probes ◽  
The One ◽  
Reporter Group ◽  
Rna And Dna

Fluorogenic hybridization probes allow the detection of RNA and DNA sequences in homogeneous solution. Typically, one target molecule is activating the fluorescence of a single probe molecule. This limits the sensitivity of nucleic acid detection. Herein, we report a self-immolative Molecular Beacon (iMB), which escapes the one-target-one-probe dogma. The iMB probe includes a photoreductively cleavable N-alkylpicolinium (NAP) linkage within the loop region. A fluorophore at the 5'-end serves, on the one hand, as a reporter group and, on the other hand, as a photosensitizer of a NAP-linker cleavage reaction. In the absence of a target, the iMB adopts a hairpin shape. Quencher proups prevent photo-induced cleavage. The iMB opens upon hybridization with target, and both fluorescent emission as well as photo-inductive cleavage of the NAP-linker can occur. In contrast to previous chemical amplification probes, iMBs are unimolecular. Cleavage leads to products that have lower target affinity than the probes before reaction. Aided by catalysis, the method allowed the detection of 5 pM RNA target within 100 min. <br>

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