Combination of peptide nucleic acid beacon and nuclease S1 for clear-cut genotyping of single nucleotide polymorphisms

2007 ◽  
Vol 363 (2) ◽  
pp. 300-302 ◽  
Author(s):  
Sheng Ye ◽  
Yoshitaka Miyajima ◽  
Toshiyuki Ohnishi ◽  
Yoji Yamamoto ◽  
Makoto Komiyama
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Peptide Nucleic Acid ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Clear Cut ◽  
Nuclease S1

scholarly journals A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Jing Zhang ◽  
Huizhe Wu ◽  
Qiuchen Chen ◽  
Pengfei Zhao ◽  
Haishan Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Room Temperature ◽  
Genetic Mutation ◽  
Locked Nucleic Acid ◽  
Nucleotide Polymorphisms ◽  
Buffer Solution ◽  
Single Nucleotide ◽  
Real Time Quantitative Pcr ◽  
Pcr Products

Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.

Locked nucleic acid (LNA) induced effect on the hybridization and fluorescence properties of oligodeoxyribonucleotides modified with nucleobase-functionalized DNA monomers

2015 ◽  
Vol 13 (26) ◽  
pp. 7236-7247 ◽  
Author(s):  
Mamta Kaura ◽  
Patrick J. Hrdlicka
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Locked Nucleic Acid ◽  
Fluorescence Properties ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Mixmer oligonucleotides modified with LNA and C5-pyrene-functionalized DNA monomers are shown to display interesting fluorescence properties for the discrimination of single nucleotide polymorphisms (SNPs).

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