Modified His-tag fusion vector for enhanced protein purification by immobilized metal affinity chromatography

2006 ◽  
Vol 355 (1) ◽  
pp. 148-150 ◽  
Author(s):  
Sinéad T. Loughran ◽  
Noeleen B. Loughran ◽  
Barry J. Ryan ◽  
Brendan N. D’Souza ◽  
Dermot Walls
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Immobilized Metal Affinity Chromatography ◽  
Metal Affinity ◽  
His Tag

scholarly journals Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

RSC Advances ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 8718-8726 ◽  
Author(s):  
Kouroush Salimi ◽  
Duygu Deniz Usta ◽  
İlkay Koçer ◽  
Eda Çelik ◽  
Ali Tuncel
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Affinity Purification ◽  
Porous Silica ◽  
Immobilized Metal Affinity Chromatography ◽  
Silica Microspheres ◽  
Magnetic Sorbent ◽  
Metal Affinity ◽  
Composite Microspheres

A magnetic sorbent based on monodisperse-porous silica microspheres was developed for His-tagged protein purification by immobilized metal affinity chromatography.

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