Protein Purification via Aqueous Two-Phase Extraction (ATPE) and Immobilized Metal Affinity Chromatography. Effectiveness of Salt Addition To Enhance Selectivity and Yield of GFPuv

2002 ◽  
Vol 18 (5) ◽  
pp. 1054-1059 ◽  
Author(s):  
Y. Li ◽  
R.R. Beitle
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Immobilized Metal Affinity Chromatography ◽  
Phase Extraction ◽  
Two Phase ◽  
Metal Affinity ◽  
Salt Addition ◽  
Aqueous Two Phase Extraction

scholarly journals Highly selective magnetic affinity purification of histidine-tagged proteins by Ni2+ carrying monodisperse composite microspheres

RSC Advances ◽  
2017 ◽  
Vol 7 (14) ◽  
pp. 8718-8726 ◽  
Author(s):  
Kouroush Salimi ◽  
Duygu Deniz Usta ◽  
İlkay Koçer ◽  
Eda Çelik ◽  
Ali Tuncel
Keyword(s):  
Affinity Chromatography ◽  
Protein Purification ◽  
Affinity Purification ◽  
Porous Silica ◽  
Immobilized Metal Affinity Chromatography ◽  
Silica Microspheres ◽  
Magnetic Sorbent ◽  
Metal Affinity ◽  
Composite Microspheres

A magnetic sorbent based on monodisperse-porous silica microspheres was developed for His-tagged protein purification by immobilized metal affinity chromatography.

Two-Step Purification of Antibody from Tobacco Plants for Vaccine Manufacturing: Aqueous Two-Phase Extraction and Affinity Chromatography

2015 ◽  
Vol 14 (1) ◽  
pp. 43-48
Author(s):  
Williams Ferro ◽  
Tatiana Álvarez ◽  
Déborah Geada ◽  
Yenisley Medina ◽  
Yarysel Guevara ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Phase Extraction ◽  
Two Phase ◽  
Tobacco Plants ◽  
Aqueous Two Phase Extraction
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