Methylation-dependent fragment separation: Direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA

2006 ◽  
Vol 354 (2) ◽  
pp. 266-273 ◽  
Author(s):  
Victoria L. Boyd ◽  
Kristina I. Moody ◽  
Achim E. Karger ◽  
Kenneth J. Livak ◽  
Gerald Zon ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Capillary Electrophoresis ◽  
Genomic Dna ◽  
Direct Detection ◽  
Pcr Products

scholarly journals Fluorescence Polarization Combined Capillary Electrophoresis Immunoassay for the Sensitive Detection of Genomic DNA Methylation

2009 ◽  
Vol 81 (19) ◽  
pp. 7885-7891 ◽  
Author(s):  
Xiaoli Wang ◽  
Yuling Song ◽  
Maoyong Song ◽  
Zhixin Wang ◽  
Tao Li ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Capillary Electrophoresis ◽  
Fluorescence Polarization ◽  
Genomic Dna ◽  
Sensitive Detection

Effect of low temperature on genomic DNA methylation in Nile tilapia (Oreochromis niloticus)

2013 ◽  
Vol 37 (10) ◽  
pp. 1460 ◽  
Author(s):  
Huaping ZHU ◽  
Maixin LU ◽  
Zhanghan HUANG ◽  
Fengying GAO ◽  
Xiaoli KE ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Low Temperature ◽  
Oreochromis Niloticus ◽  
Genomic Dna ◽  
Nile Tilapia ◽  
Nile Tilapia Oreochromis Niloticus

scholarly journals Effects of Particulate Matter on Genomic DNA Methylation Content and iNOS Promoter Methylation

2009 ◽  
Vol 117 (2) ◽  
pp. 217-222 ◽  
Author(s):  
Letizia Tarantini ◽  
Matteo Bonzini ◽  
Pietro Apostoli ◽  
Valeria Pegoraro ◽  
Valentina Bollati ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Particulate Matter ◽  
Promoter Methylation ◽  
Genomic Dna ◽  
Inos Promoter

scholarly journals Epigenetic Basis of Regeneration: Analysis of Genomic DNA Methylation Profiles in the MRL/MpJ Mouse

DNA Research ◽  
2013 ◽  
Vol 20 (6) ◽  
pp. 605-621 ◽  
Author(s):  
B. Gornikiewicz ◽  
A. Ronowicz ◽  
J. Podolak ◽  
P. Madanecki ◽  
A. Stanislawska-Sachadyn ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genomic Dna

scholarly journals EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis

2018 ◽  
Vol 221 (1) ◽  
pp. 261-278 ◽  
Author(s):  
Francesca Tedeschi ◽  
Paride Rizzo ◽  
Bui Thi Mai Huong ◽  
Andreas Czihal ◽  
Twan Rutten ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Transcription Factors ◽  
Genomic Dna

scholarly journals Physical activity and global genomic DNA methylation in a cancer-free population

Epigenetics ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Fang Fang Zhang ◽  
Roberto Cardarelli ◽  
Joan Carroll ◽  
Shun Zhang ◽  
Kimberly G. Fulda ◽  
...  
Keyword(s):  
Physical Activity ◽  
Dna Methylation ◽  
Genomic Dna

scholarly journals Folate depletion during pregnancy and lactation reduces genomic DNA methylation in murine adult offspring

2010 ◽  
Vol 6 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Jill A. McKay ◽  
Kevin J. Waltham ◽  
Elizabeth A. Williams ◽  
John C. Mathers
Keyword(s):  
Dna Methylation ◽  
Genomic Dna ◽  
Adult Offspring ◽  
Pregnancy And Lactation ◽  
Folate Depletion

scholarly journals High ferritin and low folate increases PBMCs genomic DNA methylation in association with SHMT1–1420TT variant

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Sara Moruzzi ◽  
Patrizia Guarini ◽  
Silvia Udali ◽  
Oliviero Olivieri ◽  
Domenico Girelli ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genomic Dna

scholarly journals DNA Cytosine-Demethylating Agent 5-Aza-2'-Deoxycytidine Targets Leukemia Cells through Reducing DNA N6-Methyladenine

Blood ◽  
2019 ◽  
Vol 134 (Supplement_1) ◽  
pp. 2513-2513
Author(s):  
Xiaochang Liu ◽  
Jiuxia Pang ◽  
Christopher Seiler ◽  
Ryan Kempen ◽  
Hao Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cell Proliferation ◽  
Mayo Clinic ◽  
Cell Apoptosis ◽  
Genomic Dna ◽  
Cytosine Methylation ◽  
Methylation Status ◽  
Cell Counting ◽  
Leukemia Cells ◽  
Dna Cytosine Methylation

Introduction: It is known that overexpression of DNA methyltransferases (e.g., DNMT1) is frequent and changes of DNA cytosine methylation (5mC) are a constant feature of cancers. DNA methylation inhibitors, such as 5-aza-2'-deoxycytidine (Dec), have been in clinics for patients with leukemia. It is classically believed that promoter hypomethylation coupled by reexpression of epigenetically-suppressed tumor suppressors is a core mechanism behind Dec-impaired leukemia cell growth. However, the fact that global DNA methylation profiling barely predicts Dec-response suggests a demethylation-independent mechanism of Dec-induced cell death. N6-methyladenine (m6A) has been identified recently as an abundant DNA modification in eukaryotes (Wu, Nature 2016;532:329). Importantly, m6A is extensively present in the human genome, and m6A abundance is associated with tumorigenesis (Xie, Cell 2018;71:306). Furthermore, the DNA m6A is dynamically modulated by the methyltransferases (i.e., METTL3, N6AMT1) and demethylases (i.e., ALKBH1), and changes in m6A predict gene expression (Wu, Nature 2016;532:329). Given a potential crosstalk between m6A and distinct epigenetic mechanisms (Yao, Nat. Commun 2017;8:1122), we hypothesized that the anticancer actions of Dec may partially result from changes in DNA m6A in leukemia cells. Methods: Protein expression of target genes was assessed by Western blotting. The levels of DNA cytosine methylation (5mC) and N6-methyladenine (m6A) were measured by dotblotting or liquid chromatography-mass spectrometry (LC-MS/MS). The cell viability and apoptosis were determined by the Cell Counting Kit 8 (CCK8) assays as well as the Annexin V/Propidium Iodide staining and flow cytometry. The peripheral blood mononuclear cells (PBMCs) of leukemia patients from Mayo Clinic were prepared by Ficoll-Hypaque gradient centrifugation. Results: To test our hypothesis, leukemia cells, Kasumi-1, MV4-11, K562 and KU812, were exposed to 2 µM Dec, a clinical achievable concentration, for 72 hours. As expected, Dec treatment led to a downregulation of DNMT1 and DNMT3a, a reduction of 5mC levels by dotblotting using anti-5mC antibody, a blockage of cell proliferation and a promotion of cell apoptosis. When genomic DNA was subjected to dotblotting using anti-m6A antibody, the results revealed a marked decrease of DNA m6A levels in all Dec-treated cells. Then genomic DNA from K562 and MV4-11 cells was enzymatically digested to 2'-deoxynucleosides. dA was quantified by HPLC-UV, while the amount of m6A was measured by isotope dilution HPLC-ESI-MS/MS using 15N labeled internal standard. The standard curves were generated using pure standards, from which the m6A/A ratio was calculated. In agreement with dotblotting results, Dec treatment significantly decreased DNA m6A abundance in both cell lines. Mechanistically, exposure to Dec led to a consistent increase of demethylase fat mass and obesity-associated protein (FTO), but not METTL3 nor ALKBH1 and ALKBH5. Further, knockdown of FTO increased DNA m6A, which was further confirmed by treatment with FTO inhibitors rhein and meclofenamic acid (MA). These data indicate that FTO may be responsible for Dec-induced m6A changes in leukemia cells. To investigate the clinical implications of DNA m6A, we obtained PBMCs from AML patients (n = 10), who received Dec therapy (20 mg/m2 daily for 5 days every 4 weeks) in Mayo Clinic. These PBMCs were further cultured for 48 hours, frozen and stored in 100% ethanol before DNA extraction. The results from dotblotting using anti-5mC or anti-m6A showed that a trend of decrease in both m6A and 5mC abundance is observed, and the pattern of changes in m6A and 5mC displays a positive correlation. Finally, exposure of leukemia cells to the combination of Dec (2 µM) with FTO inhibitor MA (50 µM) induced more cell apoptosis and greater inhibition on cell proliferation as compared to single agent in vitro, supporting FTO inhibitors as new therapeutic agents in leukemia. Conclusion: Our studies suggest that the FTO-DNA m6A axis may partially mediate the therapeutic outcomes of Dec in leukemia. Our findings provide a new mechanistic paradigm for the anticancer activities of Dec, and define the m6A methylation status in leukemia cells as a new pharmacodynamic marker for their response to Dec-based therapy, pointing to a novel treatment strategy for incorporating m6A modulators to enhance the therapeutic index of Dec. Disclosures Al-Kali: Astex Pharmaceuticals, Inc.: Research Funding.

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