Synthesis of photoaffinity probes [2′-iodo-4′-(3′′-trifluoromethyldiazirinyl)phenoxy]-d-glucopyranoside and [(4′-benzoyl)phenoxy]-d-glucopyranoside for the identification of sugar-binding and phlorizin-binding sites in the sodium/d-glucose cotransporter protein

2003 ◽  
Vol 323 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Navneet Kumar Tyagi ◽  
Rolf K.H Kinne
Keyword(s):  
Binding Sites ◽  
Sugar Binding ◽  
Phlorizin Binding ◽  
Photoaffinity Probes

scholarly journals Structure of uPAR, plasminogen, and sugar-binding sites of the 300 kDa mannose 6-phosphate receptor

2004 ◽  
Vol 23 (10) ◽  
pp. 2019-2028 ◽  
Author(s):  
Linda J Olson ◽  
Rama D Yammani ◽  
Nancy M Dahms ◽  
Jung-Ja P Kim
Keyword(s):  
Binding Sites ◽  
Sugar Binding ◽  
Mannose 6 Phosphate

Evidence for a Role of Helix IV in Connecting Cation- and Sugar-Binding Sites ofEscherichia coliMelibiose Permease†

Biochemistry ◽  
2000 ◽  
Vol 39 (15) ◽  
pp. 4493-4499 ◽  
Author(s):  
Emmanuelle Cordat ◽  
Gérard Leblanc ◽  
Isabelle Mus-Veteau
Keyword(s):  
Binding Sites ◽  
Sugar Binding

O1-11-01: Gamma-secretase inhibitor (GSI) and modulator (GSM) photoaffinity probes reveal distinct binding sites on presenilin

2013 ◽  
Vol 9 ◽  
pp. P151-P151
Author(s):  
Doug Johnson ◽  
Kelly Bales ◽  
Nikolay Pozdnyakov ◽  
Heather Murrey ◽  
Martin Pettersson ◽  
...  
Keyword(s):  
Binding Sites ◽  
Gamma Secretase ◽  
Secretase Inhibitor ◽  
Photoaffinity Probes ◽  
Gamma Secretase Inhibitor

scholarly journals Analysis of the structural requirements of sugar binding to the liver, brain and insulin-responsive glucose transporters expressed in oocytes

1993 ◽  
Vol 294 (3) ◽  
pp. 753-760 ◽  
Author(s):  
C A Colville ◽  
M J Seatter ◽  
G W Gould
Keyword(s):  
Hydrogen Bond ◽  
Binding Sites ◽  
Glucose Transporters ◽  
Binding Pocket ◽  
Structural Basis ◽  
Sugar Binding ◽  
Glucose Analogues ◽  
Sugar Recognition

We have expressed the liver (GLUT 2), brain (GLUT 3) and insulin-responsive (GLUT 4) glucose transporters in oocytes from Xenopus laevis by microinjection of in vitro-transcribed mRNA. Using a range of halogeno- and deoxy-glucose analogues, and other hexoses, we have studied the structural basis of sugar binding to these different isoforms. We show that a hydrogen bond to the C-3 position is involved in sugar binding for all three isoforms, but that the direction of this hydrogen bond is different in GLUT 2 from either GLUT 1, 3 or 4. Hydrogen-bonding at the C-4 position is also involved in sugar recognition by all three isoforms, but we propose that in GLUT 3 this hydrogen bond plays a less significant role than in GLUT 2 and 4. In all transporters we propose that the C-4 position is directed out of the sugar-binding pocket. The role of the C-6 position is also discussed. In addition, we have analysed the ability of fructopyranose and fructofuranose analogues to inhibit the transport mediated by GLUT2. We show that fructofuranose analogues, but not fructopyranose analogues, are efficient inhibitors of transport mediated by GLUT 2, and therefore suggest that GLUT 2 accommodates D-glucose as a pyranose ring, but D-fructose as a furanose ring. Models for the binding sites of GLUT 2, 3 and 4 are presented.

In situ ultrastructural localization of sugar-binding sites in lizard granulosa cell nuclei

1985 ◽  
Vol 55 (1-2) ◽  
pp. 15-20 ◽  
Author(s):  
J. Hubert ◽  
A. P. Seve ◽  
D. Bouvier ◽  
C. Masson ◽  
M. Bouteille ◽  
...  
Keyword(s):  
Granulosa Cell ◽  
Binding Sites ◽  
Ultrastructural Localization ◽  
Cell Nuclei ◽  
Sugar Binding

Net sugar transport is a multistep process. Evidence for cytosolic sugar binding sites in erythrocytes

Biochemistry ◽  
1995 ◽  
Vol 34 (47) ◽  
pp. 15395-15406 ◽  
Author(s):  
Erin K. Cloherty ◽  
Lisa A. Sultzman ◽  
Ralph J. Zottola ◽  
Anthony Carruthers
Keyword(s):  
Binding Sites ◽  
Sugar Transport ◽  
Sugar Binding ◽  
Multistep Process

Multiple sugar binding sites in α-glucosidase

2003 ◽  
Vol 1645 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Xiaojie Yao ◽  
Rebecca Mauldin ◽  
Larry Byers
Keyword(s):  
Binding Sites ◽  
Sugar Binding

scholarly journals Interaction of Azospirillum lipoferumwith Wheat Germ Agglutinin Stimulates Nitrogen Fixation

1999 ◽  
Vol 181 (13) ◽  
pp. 3949-3955 ◽  
Author(s):  
Eva Karpati ◽  
Peter Kiss ◽  
Tamas Ponyi ◽  
Istvan Fendrik ◽  
Miklos de Zamaroczy ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Binding Sites ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Side Chains ◽  
Receptor Complex ◽  
Sugar Binding ◽  
Signalling Cascade

ABSTRACT In vitro, the nitrogen fixation capability of A. lipoferum is efficiently increased in the presence of wheat germ agglutinin (WGA). A putative WGA-binding receptor, a 32-kDa protein, was detected in the cell capsule. The stimulatory effect requiredN-acetyl-d-glucosamine dimer (GlcNAcdi) terminated sugar side chains of the receptor and was dependent on the number of GlcNAcdi links involved in receptor-WGA interface. Binding to the primary sugar binding sites on WGA had a larger stimulatory effect than binding to the secondary sites. The WGA-receptor complex generated stimulus led to elevated transcription of the nifH and nifA genes and of the glnBA gene cluster but not of the glnA gene from its own promoter. There may well be a signalling cascade contributing to the regulation of nitrogen fixation.

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