General Genetic Strategies

2018 ◽  
pp. 113-132 ◽  
Author(s):  
Louise M. Steele ◽  
Philip G. Morgan
Keyword(s):  
Genetic Strategies

Recent Patents on Melanoma with Focus on Genetic Strategies

2008 ◽  
Vol 3 (2) ◽  
pp. 136-144
Author(s):  
Talia Gramiccia ◽  
Rosita Saraceno ◽  
Alessandro Stefani ◽  
Sergio Chimenti ◽  
Alessandro Stefani
Keyword(s):  
Genetic Strategies

15-Lipoxygenase-2 Deficiency Induces a Dysfunction in Macrophages That Can Be Restored by Salidroside

2021 ◽  
Author(s):  
Rong Huang Huang ◽  
Tingting Li Li ◽  
Xi Yong Yong ◽  
Huling Wen Wen ◽  
Xing Zhou Zhou ◽  
...  
Keyword(s):  
Natural Product ◽  
Signaling Pathway ◽  
Therapeutic Strategy ◽  
Caspase 3 ◽  
Mechanisms Of Action ◽  
Rna Seq ◽  
Immunological Function ◽  
Tnf Α ◽  
And Function ◽  
Genetic Strategies

Abstract 15-Lipoxygenase-2(15-LOX-2) is thought to regulate inflammation and immunological function however, its mechanisms of action are still unclear. Furthermore, it has been reported that salidroside has anti inflammatory properties , but its role in macrophage function has not been understood yet In this study, we aimed to determine how 15-LOX-2 expression level s affect the function of macrophages and the effect of salidroside on 15-LOX-2 deficient macrophages We used multiple functional genetic strategies to determine 15-LOX-2 function in macrophages. 15-LOX-2 deficiency promotes phagocytosis and proliferation of macrophages and impairs their apoptosis Mechanistically, t he expression levels of cyclophilinB (CypB) were upregulated in 15-LOX-2 deficient Ana 1 macrophages, whereas those of caspase 3 were down regulated. Furthermore, RNA-seq analysis showed that inflammation, complement, and TNF-α signaling pathway s were all activated in 15-LOX-2 deficient Ana 1 macrophages. Treatment of 15-LOX-2 deficient macrophages with salidroside, a natural product derived from Rhodiola species, effectively reversed the effects of 15-LOX-2 deficiency on caspase 3 and CypB levels, as well as on apoptosis and proliferation. In conclusion, our study shows that there is a newly identified link between 15-LOX-2 deficiency and salidroside in regulating macrophage survival, proliferation, and function. Salidroside may be a promising therapeutic strategy for treating inflammation related diseases resulting from 15-LOX-2 deficiency.

Genetic Strategies to Understand Human Diabetic Nephropathy: Wet-Lab Approaches

2019 ◽  
pp. 205-240
Author(s):  
Laura J. Smyth ◽  
Katie Kerr ◽  
Seamus Duffy ◽  
Jill Kilner ◽  
Amy Jayne McKnight
Keyword(s):  
Diabetic Nephropathy ◽  
Wet Lab ◽  
Genetic Strategies

Panel Discussion: Comparison of Traditional and Genetic Strategies

1984 ◽  
pp. 267-273
Author(s):  
Ross D. Brown ◽  
Joel S. Hirschhorn ◽  
Hester A. Kobayashi ◽  
Joseph L. McCarthy ◽  
James W. Patterson ◽  
...  
Keyword(s):  
Panel Discussion ◽  
Genetic Strategies

scholarly journals Genetically Encoded CRISPR components Yield Efficient Gene Editing in the Invasive Pest,Drosophila suzukii

2021 ◽  
Author(s):  
Nikolay P. Kandul ◽  
Esther J. Belikoff ◽  
Junru Liu ◽  
Anna Buchman ◽  
Fang Li ◽  
...  
Keyword(s):  
Population Control ◽  
Invasive Pest ◽  
Drosophila Suzukii ◽  
Guide Rnas ◽  
Cas9 Nuclease ◽  
Efficient Gene ◽  
Transgenic Lines ◽  
Repeated Applications ◽  
Very High ◽  
Genetic Strategies

AbstractOriginally from Asia,Drosophila suzukii(Matsumura, 1931, Diptera:Drosophilidae) is presently a global pest of economically important soft-skinned fruits. Also commonly known as spotted wingDrosophila(SWD), it is largely controlled through repeated applications of broad-spectrum insecticides. There is a pressing need for a better understanding of SWD biology and for developing alternative environmentally-friendly methods of control. The RNA-guided Cas9 nuclease has revolutionized functional genomics and is an integral component of several recently developed genetic strategies for population control of insects. Here we have developed transgenic strains that encode three different terminators and four different promoters to express Cas9 in both the soma and/or germline of SWD. The Cas9 lines were evaluated through genetic crossing to transgenic lines that encode single guide RNAs targeting the conserved X-linkedyellowbody andwhiteeye genes. We find that several Cas9/gRNA lines display very high editing capacity. Going forward, these tools will be instrumental for evaluating gene function in SWD and may provide tools useful for the development of new genetic strategies for control of this invasive species.

Genetic strategies to determine the mode of 2n egg formation in diploid potatoes

Euphytica ◽  
1988 ◽  
Vol 38 (3) ◽  
pp. 247-260 ◽  
Author(s):  
D. S. Douches ◽  
C. F. Quiros
Keyword(s):  
Egg Formation ◽  
Genetic Strategies
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