Tomato Fruits—A Platform for Metabolic Engineering of Terpenes

2016 ◽  
pp. 333-359 ◽  
Author(s):  
M. Gutensohn ◽  
N. Dudareva
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits

scholarly journals Metabolic engineering of xanthophyll content in tomato fruits

FEBS Letters ◽  
2002 ◽  
Vol 519 (1-3) ◽  
pp. 30-34 ◽  
Author(s):  
Sridhar Dharmapuri ◽  
Carlo Rosati ◽  
Patrizia Pallara ◽  
Riccardo Aquilani ◽  
Florence Bouvier ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits

scholarly journals Enhanced Levels of the Aroma and Flavor Compound S-Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits

2001 ◽  
Vol 127 (3) ◽  
pp. 1256-1265 ◽  
Author(s):  
Efraim Lewinsohn ◽  
Fernond Schalechet ◽  
Jack Wilkinson ◽  
Kenji Matsui ◽  
Yaakov Tadmor ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits ◽  
Flavor Compound ◽  
Terpenoid Pathway

scholarly journals Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits

2014 ◽  
Vol 31 (5) ◽  
pp. 567-571 ◽  
Author(s):  
Takashi Kawasaki ◽  
Takao Koeduka ◽  
Akifumi Sugiyama ◽  
Kanako Sasaki ◽  
Philip J. Linley ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Chalcone Isomerase ◽  
Tomato Fruits

scholarly journals Altered Levels of Aroma and Volatiles by Metabolic Engineering of Shikimate Pathway Genes in Tomato Fruits

2015 ◽  
Vol 2 (2) ◽  
pp. 75-92 ◽  
Author(s):  
Vered Tzin ◽  
◽  
Ilana Rogachev ◽  
Sagit Meir ◽  
Michal Moyal Ben Zvi ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Shikimate Pathway ◽  
Tomato Fruits

Metabolic engineering of monoterpene biosynthesis in tomato fruits via introduction of the non-canonical substrate neryl diphosphate

2014 ◽  
Vol 24 ◽  
pp. 107-116 ◽  
Author(s):  
Michael Gutensohn ◽  
Thuong T.H. Nguyen ◽  
Richard D. McMahon ◽  
Ian Kaplan ◽  
Eran Pichersky ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits ◽  
Monoterpene Biosynthesis

scholarly journals Overcoming Bottlenecks for Metabolic Engineering of Sesquiterpene Production in Tomato Fruits

2021 ◽  
Vol 12 ◽  
Author(s):  
Michael Gutensohn ◽  
Laura K. Henry ◽  
Scott A. Gentry ◽  
Joseph H. Lynch ◽  
Thuong T. H. Nguyen ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Metabolic Flux ◽  
Biotic Interactions ◽  
Fold Increase ◽  
Carotenoid Biosynthesis ◽  
Industrial Applications ◽  
Plant Metabolites ◽  
Terpene Synthases ◽  
Tomato Fruits ◽  
Indirect Determination

Terpenoids are a large and diverse class of plant metabolites that also includes volatile mono- and sesquiterpenes which are involved in biotic interactions of plants. Due to the limited natural availability of these terpenes and the tight regulation of their biosynthesis, there is strong interest to introduce or enhance their production in crop plants by metabolic engineering for agricultural, pharmaceutical and industrial applications. While engineering of monoterpenes has been quite successful, expression of sesquiterpene synthases in engineered plants frequently resulted in production of only minor amounts of sesquiterpenes. To identify bottlenecks for sesquiterpene engineering in plants, we have used two nearly identical terpene synthases, snapdragon (Antirrhinum majus) nerolidol/linalool synthase-1 and -2 (AmNES/LIS-1/-2), that are localized in the cytosol and plastids, respectively. Since these two bifunctional terpene synthases have very similar catalytic properties with geranyl diphosphate (GPP) and farnesyl diphosphate (FPP), their expression in target tissues allows indirect determination of the availability of these substrates in both subcellular compartments. Both terpene synthases were expressed under control of the ripening specific PG promoter in tomato fruits, which are characterized by a highly active terpenoid metabolism providing precursors for carotenoid biosynthesis. As AmNES/LIS-2 fruits produced the monoterpene linalool, AmNES/LIS-1 fruits were found to exclusively produce the sesquiterpene nerolidol. While nerolidol emission in AmNES/LIS-1 fruits was 60- to 584-fold lower compared to linalool emission in AmNES/LIS-2 fruits, accumulation of nerolidol-glucosides in AmNES/LIS-1 fruits was 4- to 14-fold lower than that of linalool-glucosides in AmNES/LIS-2 fruits. These results suggest that only a relatively small pool of FPP is available for sesquiterpene formation in the cytosol. To potentially overcome limitations in sesquiterpene production, we transiently co-expressed the key pathway-enzymes hydroxymethylglutaryl-CoA reductase (HMGR) and 1-deoxy-D-xylulose 5-phosphate synthase (DXS), as well as the regulator isopentenyl phosphate kinase (IPK). While HMGR and IPK expression increased metabolic flux toward nerolidol formation 5.7- and 2.9-fold, respectively, DXS expression only resulted in a 2.5-fold increase.

Metabolic Engineering for Functional Foods: Tomato Fruits and Stilbenes

2013 ◽  
pp. 1581-1597
Author(s):  
Giovanna Giovinazzo ◽  
Ilaria Ingrosso ◽  
Marco Taurino ◽  
Angelo Santino
Keyword(s):  
Metabolic Engineering ◽  
Functional Foods ◽  
Tomato Fruits

Metabolic engineering of CHO cells to prepare glycoproteins

2018 ◽  
Vol 2 (3) ◽  
pp. 433-442 ◽  
Author(s):  
Qiong Wang ◽  
Michael J. Betenbaugh
Keyword(s):  
Metabolic Engineering ◽  
Cho Cells ◽  
Genetic Manipulation ◽  
Chinese Hamster ◽  
Post Translational Modification ◽  
Effector Functions ◽  
Recombinant Glycoproteins ◽  
Production Platform ◽  
Chemical Inhibitors ◽  
Amino Acid Mutations

As a complex and common post-translational modification, N-linked glycosylation affects a recombinant glycoprotein's biological activity and efficacy. For example, the α1,6-fucosylation significantly affects antibody-dependent cellular cytotoxicity and α2,6-sialylation is critical for antibody anti-inflammatory activity. Terminal sialylation is important for a glycoprotein's circulatory half-life. Chinese hamster ovary (CHO) cells are currently the predominant recombinant protein production platform, and, in this review, the characteristics of CHO glycosylation are summarized. Moreover, recent and current metabolic engineering strategies for tailoring glycoprotein fucosylation and sialylation in CHO cells, intensely investigated in the past decades, are described. One approach for reducing α1,6-fucosylation is through inhibiting fucosyltransferase (FUT8) expression by knockdown and knockout methods. Another approach to modulate fucosylation is through inhibition of multiple genes in the fucosylation biosynthesis pathway or through chemical inhibitors. To modulate antibody sialylation of the fragment crystallizable region, expressions of sialyltransferase and galactotransferase individually or together with amino acid mutations can affect antibody glycoforms and further influence antibody effector functions. The inhibition of sialidase expression and chemical supplementations are also effective and complementary approaches to improve the sialylation levels on recombinant glycoproteins. The engineering of CHO cells or protein sequence to control glycoforms to produce more homogenous glycans is an emerging topic. For modulating the glycosylation metabolic pathways, the interplay of multiple glyco-gene knockouts and knockins and the combination of multiple approaches, including genetic manipulation, protein engineering and chemical supplementation, are detailed in order to achieve specific glycan profiles on recombinant glycoproteins for superior biological function and effectiveness.

Study of the quality and antioxidant properties of tomatoes (Solanum lycopersicum L.) under different postharvest and dehydration conditions

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
María Lorena Luna-Guevara ◽  
Teresita González-Sánchez ◽  
Adriana Delgado-Alvarado ◽  
María Elena Ramos-Cassellis ◽  
José Guillermo Pérez-Luna ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rural Communities ◽  
Antioxidant Properties ◽  
Titratable Acidity ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Convective Drying ◽  
Tomato Fruits ◽  
Solanum Lycopersicum L ◽  
Storage Temperatures

Objective: To study the effect of storage temperatures and dehydration conditions (solar and convective drying; SD, CD), on the quality, physicochemical parameters and antioxidant properties of tomato fruits. Methodology: The physicochemical characteristics pH, titratable acidity, soluble solids (°Bx) and color parameters (L*, a* and b*), were evaluated. The lycopene, carotenoids and antioxidant activity percentages retention of tomatoes fruits stored at 7 and 22 ° C for 5 days and subjected to SD (Temperature (T) of 67 °C and luminescence of 685 lum/sqf) and CD (T 70 °C, flow rates 0.5, 1.0 and 1.5 m/s), were analyzed. Results: The fruits reached humidities of 17 and 15% for SD and CD. The parameters pH, °Bx, L*, a*, b* were highest with 22°C and CD (1.5 m/s). The value of the carotenoids was higher in fruits stored at 7 ° C and subjected to CD (1.0 and 1.5 m/s) and SD with values of 83.85, 85.98 and 99.43%, respectively. The CD (0.5 m/s) and SD improved lycopene (94.37 and 95.14%) and the antioxidant activity with values of 73.06 and 97.21%. Implications: The application of solar dehydration depends on luminescence condition; however, it is inexpensive and environmentally friendly alternative. Conclusions: The results derived in a viable alternative for the conservation and commercialization of tomato fruits in rural communities

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