scholarly journals Altered Levels of Aroma and Volatiles by Metabolic Engineering of Shikimate Pathway Genes in Tomato Fruits

2015 ◽  
Vol 2 (2) ◽  
pp. 75-92 ◽  
Author(s):  
Vered Tzin ◽  
◽  
Ilana Rogachev ◽  
Sagit Meir ◽  
Michal Moyal Ben Zvi ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Shikimate Pathway ◽  
Tomato Fruits

scholarly journals Metabolic engineering of Escherichia coli for production of chemicals derived from the shikimate pathway

2020 ◽  
Vol 47 (6-7) ◽  
pp. 525-535
Author(s):  
Zhu Li ◽  
Huiying Wang ◽  
Dongqin Ding ◽  
Yongfei Liu ◽  
Huan Fang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Shikimate Pathway

scholarly journals Metabolic engineering of xanthophyll content in tomato fruits

FEBS Letters ◽  
2002 ◽  
Vol 519 (1-3) ◽  
pp. 30-34 ◽  
Author(s):  
Sridhar Dharmapuri ◽  
Carlo Rosati ◽  
Patrizia Pallara ◽  
Riccardo Aquilani ◽  
Florence Bouvier ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits

scholarly journals Enhanced Levels of the Aroma and Flavor Compound S-Linalool by Metabolic Engineering of the Terpenoid Pathway in Tomato Fruits

2001 ◽  
Vol 127 (3) ◽  
pp. 1256-1265 ◽  
Author(s):  
Efraim Lewinsohn ◽  
Fernond Schalechet ◽  
Jack Wilkinson ◽  
Kenji Matsui ◽  
Yaakov Tadmor ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Tomato Fruits ◽  
Flavor Compound ◽  
Terpenoid Pathway

scholarly journals Coenzyme Q10 Biosynthesis Established in the Non-Ubiquinone Containing Corynebacterium glutamicum by Metabolic Engineering

2021 ◽  
Vol 9 ◽  
Author(s):  
Arthur Burgardt ◽  
Ayham Moustafa ◽  
Marcus Persicke ◽  
Jens Sproß ◽  
Thomas Patschkowski ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
Paracoccus Denitrificans ◽  
Coenzyme Q ◽  
Shikimate Pathway ◽  
Farnesyl Diphosphate ◽  
Mass Spectrometry Analysis ◽  
Biotechnological Production ◽  
Spectrometry Analysis ◽  
Shake Flask Cultivation

Coenzyme Q10 (CoQ10) serves as an electron carrier in aerobic respiration and has become an interesting target for biotechnological production due to its antioxidative effect and benefits in supplementation to patients with various diseases. For the microbial production, so far only bacteria have been used that naturally synthesize CoQ10 or a related CoQ species. Since the whole pathway involves many enzymatic steps and has not been fully elucidated yet, the set of genes required for transfer of CoQ10 synthesis to a bacterium not naturally synthesizing CoQ species remained unknown. Here, we established CoQ10 biosynthesis in the non-ubiquinone-containing Gram-positive Corynebacterium glutamicum by metabolic engineering. CoQ10 biosynthesis involves prenylation and, thus, requires farnesyl diphosphate as precursor. A carotenoid-deficient strain was engineered to synthesize an increased supply of the precursor molecule farnesyl diphosphate. Increased farnesyl diphosphate supply was demonstrated indirectly by increased conversion to amorpha-4,11-diene. To provide the first CoQ10 precursor decaprenyl diphosphate (DPP) from farnesyl diphosphate, DPP synthase gene ddsA from Paracoccus denitrificans was expressed. Improved supply of the second CoQ10 precursor, para-hydroxybenzoate (pHBA), resulted from metabolic engineering of the shikimate pathway. Prenylation of pHBA with DPP and subsequent decarboxylation, hydroxylation, and methylation reactions to yield CoQ10 was achieved by expression of ubi genes from Escherichia coli. CoQ10 biosynthesis was demonstrated in shake-flask cultivation and verified by liquid chromatography mass spectrometry analysis. To the best of our knowledge, this is the first report of CoQ10 production in a non-ubiquinone-containing bacterium.

scholarly journals Metabolic engineering of flavonoids with prenyltransferase and chalcone isomerase genes in tomato fruits

2014 ◽  
Vol 31 (5) ◽  
pp. 567-571 ◽  
Author(s):  
Takashi Kawasaki ◽  
Takao Koeduka ◽  
Akifumi Sugiyama ◽  
Kanako Sasaki ◽  
Philip J. Linley ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Chalcone Isomerase ◽  
Tomato Fruits

scholarly journals New aspects of microbial vitamin K2 production by expanding the product spectrum

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Zimeng Zhang ◽  
Linxia Liu ◽  
Chuan Liu ◽  
Yumei Sun ◽  
Dawei Zhang
Keyword(s):  
Metabolic Engineering ◽  
Small Molecules ◽  
Shikimate Pathway ◽  
Vitamin K2 ◽  
Side Chain ◽  
Head Group ◽  
Depth Information ◽  
Product Spectrum ◽  
Different Types ◽  
Membrane Engineering

AbstractVitamin K2 (menaquinone, MK) is an essential lipid-soluble vitamin with critical roles in blood coagulation and bone metabolism. Chemically, the term vitamin K2 encompasses a group of small molecules that contain a common naphthoquinone head group and a polyisoprenyl side chain of variable length. Among them, menaquinone-7 (MK-7) is the most potent form. Here, the biosynthetic pathways of vitamin K2 and different types of MK produced by microorganisms are briefly introduced. Further, we provide a new aspect of MK-7 production, which shares a common naphthoquinone ring and polyisoprene biosynthesis pathway, by analyzing strategies for expanding the product spectrum. We review the findings of metabolic engineering strategies targeting the shikimate pathway, polyisoprene pathway, and menaquinone pathway, as well as membrane engineering, which provide comprehensive insights for enhancing the yield of MK-7. Finally, the current limitations and perspectives of microbial menaquinone production are also discussed. This article provides in-depth information on metabolic engineering strategies for vitamin K2 production by expanding the product spectrum.

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