In silico model-driven cofactor engineering strategies for improving the overall NADP(H) turnover in microbial cell factories

2015 ◽  
Vol 42 (10) ◽  
pp. 1401-1414 ◽  
Author(s):  
Meiyappan Lakshmanan ◽  
Kai Yu ◽  
Lokanand Koduru ◽  
Dong-Yup Lee
Keyword(s):  
In Silico ◽  
Microbial Cell ◽  
Cofactor Engineering ◽  
Model Driven ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
In Silico Model

Genome-scale in silico modeling and analysis for designing synthetic terpenoid-producing microbial cell factories

2013 ◽  
Vol 103 ◽  
pp. 100-108 ◽  
Author(s):  
Bevan Kai-Sheng Chung ◽  
Meiyappan Lakshmanan ◽  
Maximilian Klement ◽  
Bijayalaxmi Mohanty ◽  
Dong-Yup Lee
Keyword(s):  
In Silico ◽  
Microbial Cell ◽  
Modeling And Analysis ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
In Silico Modeling ◽  
Genome Scale

Developing fourth-generation biofuels secreting microbial cell factories for enhanced productivity and efficient product recovery; a review

Fuel ◽  
2021 ◽  
Vol 298 ◽  
pp. 120858
Author(s):  
Sana Malik ◽  
Ayesha Shahid ◽  
Chen-Guang Liu ◽  
Aqib Zafar Khan ◽  
Muhammad Zohaib Nawaz ◽  
...  
Keyword(s):  
Product Recovery ◽  
Microbial Cell ◽  
Fourth Generation ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Establishing new microbial cell factories for sustainable bioprocesses

2012 ◽  
Vol 29 ◽  
pp. S75-S76
Author(s):  
Mhairi Workman ◽  
Philippe Holt ◽  
Xiaoying Liu
Keyword(s):  
Microbial Cell ◽  
Microbial Cell Factories ◽  
Cell Factories

scholarly journals Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

2018 ◽  
Vol 38 (2) ◽  
Author(s):  
Johanna Hausjell ◽  
Heidi Halbwirth ◽  
Oliver Spadiut
Keyword(s):  
Cytochrome P450s ◽  
Microbial Cell ◽  
Cytochrome P450 Reductase ◽  
Redox Partner ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Recombinant Production ◽  
Membrane Bound ◽  
Essential Reactions ◽  
Synthesis Of Hormones

Cytochrome P450s (P450s) comprise one of the largest known protein families. They occur in every kingdom of life and catalyze essential reactions, such as carbon source assimilation, synthesis of hormones and secondary metabolites, or degradation of xenobiotics. Due to their outstanding ability of specifically hydroxylating complex hydrocarbons, there is a great demand to use these enzymes for biocatalysis, including applications at an industrial scale. Thus, the recombinant production of these enzymes is intensively investigated. However, especially eukaryotic P450s are difficult to produce. Challenges are faced due to complex cofactor requirements and the availability of a redox-partner (cytochrome P450 reductase, CPR) can be a key element to get active P450s. Additionally, most eukaryotic P450s are membrane bound which complicates the recombinant production. This review describes current strategies for expression of P450s in the microbial cell factories Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris.

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