Use of Microscopic Morphology in Smears Prepared from Radiometric Cultures or Presumptive Identification of Mycobacterium tuberculosis Complex, Mycobacterium avium Complex, Mycobacterium kansasii , and Mycobacterium xenopi

1998 ◽  
Vol 17 (7) ◽  
pp. 493-500
Author(s):  
J. González ◽  
G. Tudó ◽  
J. Gómez ◽  
A. García ◽  
M. Navarro ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Complex ◽  
Mycobacterium Tuberculosis Complex ◽  
Mycobacterium Kansasii ◽  
Tuberculosis Complex ◽  
Mycobacterium Xenopi ◽  
Presumptive Identification ◽  
Microscopic Morphology

scholarly journals Growth characteristics of liquid cultures increase the reliability of presumptive identification of Mycobacterium tuberculosis complex

2018 ◽  
Vol 67 (6) ◽  
pp. 828-833 ◽  
Author(s):  
Juliana Maira Watanabe Pinhata ◽  
Isis Moreira Felippe ◽  
Juliana Failde Gallo ◽  
Erica Chimara ◽  
Lucilaine Ferrazoli ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Growth Characteristics ◽  
Tuberculosis Complex ◽  
Liquid Cultures ◽  
Presumptive Identification

scholarly journals Prevalence and Risk Factors for Pulmonary Mycobacteriosis in Lagos, Nigeria

2019 ◽  
pp. 1-12
Author(s):  
T. Y. Raheem ◽  
B. A. Iwalokun ◽  
A. Oluwadun ◽  
O. A. Adesesan ◽  
N. Tochukwu ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mycobacterium Tuberculosis ◽  
Informed Consent ◽  
Mycobacterium Avium Complex ◽  
Mycobacterium Tuberculosis Complex ◽  
Tuberculosis Complex ◽  
Cross Sectional ◽  
Mycobacterial Infections ◽  
Inappropriate Treatment ◽  
Lowenstein Jensen

Background: Pulmonary mycobacteriosis has been documented in HIV-infected, diabetics, asthmatics, smokers and alcoholics and its progression and severity are affected by these risk factors. Inappropriate diagnosis of mycobacteriosis could lead to inappropriate treatment with anti- tuberculosis drugs. Methods: This cross-sectional, prospective study was conducted in patients with TB-like diseases attending six DOTs centres in Lagos, Nigeria, from May 2012 to October 2016. Participants’ informed consent was obtained, structured questionnaires administered to obtain socio-demographic and co-morbid data. Sputum samples collected and processed for microscopy and culture using Lowenstein-Jensen medium with or without pyruvate and MGIT 960 liquid medium. Mycobacteria were identified using MPT64 immunochromatographic, biochemical and molecular methods. This study investigated the presence and prevalence of mycobacteriosis in the participants and assessed the risk factors for the mycobacterial infections. Results: Of the 1,020 participants, 339 (33.2%) had mycobacteriosis of which 33 (9.7%) were caused by Non-Tuberculosis Mycobacteria (NTM) and 306 (90.3%) caused by Mycobacterium tuberculosis complex (MTBC). Of the isolated 306 MTBC, 247 (80.7%) were M. tuberculosis, 28 (9.2%) were M. africanum, 23 (7.5%) were M. bovis while 8(2.6%) were M. ulcerans [P < 0.0005]. The 33 NTM showed 11 (33.3%), 20 (60.6%) had HIV, 8(24.2%) M. fortuitum, 2 (6.1%) M. abscessus, 2 (6.1%) M. scrofulacium, 6 (18.2%) M. kansasii, 4 (12.1%) M. megateriense and 11 (33.3%) Mycobacterium avium complex (MAC). Sequence analysis of the 16s rRNA of the 11 MAC showed 3 (27.3%) M. avium, 5(45.5%) M. intracellulare, 2(18.2%) M. colombiense and 1(9.1%) M. velneri.  M. fortuitum and MAC were significantly (P<0.05) associated with HIV infection, while only M. fortuitum relate strongly with diabetes (P <0.05). Conclusion: The study showed that mycobacteriosis is caused by different species of MTBC and NTM. Relatively high mycobacteriosis were detected during dry season and were significantly associated with gender, age, HIV and diabetes.

scholarly journals Cord formation and colony morphology for the presumptive identification of Mycobacterium tuberculosis complex

2003 ◽  
Vol 34 (2) ◽  
Author(s):  
Paulo Henrique Tasso Monteiro ◽  
Maria Conceição Martins ◽  
Suely Yoko Mizuka Ueki ◽  
Carmen Maria Saraiva Giampaglia ◽  
Maria Alice da Silva Telles
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Colony Morphology ◽  
Tuberculosis Complex ◽  
Presumptive Identification

scholarly journals Assessment of Morphology for Rapid Presumptive Identification of Mycobacterium tuberculosis andMycobacterium kansasii

2000 ◽  
Vol 38 (4) ◽  
pp. 1426-1429 ◽  
Author(s):  
Silvia Attorri ◽  
Sherry Dunbar ◽  
Jill E. Clarridge
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Avium ◽  
Cost Effective ◽  
Mycobacterium Kansasii ◽  
Specific Training ◽  
Predictive Values ◽  
Morphologic Characteristics ◽  
Cost Effective Method ◽  
Probe Selection ◽  
Presumptive Identification

Mycobacterium tuberculosis often exhibits serpentine cording when grown in liquid medium, whereas Mycobacterium kansasii can be larger and cross-barred. We assessed the use of these morphologic characteristics as a cost-effective method for rapid presumptive identification of isolates from BACTEC bottles. Without specific training, using the Kinyoun acid-fast stain, definitive cording was found in 237 of 373 specimens positive forM. tuberculosis (64%) and cross-barring was recognized within 63 of 76 (83%) of the specimens positive for M. kansasii, giving sensitivities specificities, positive predictive values, and negative predictive values of 63.5, 96, 92, and 79%, respectively, for M. tuberculosis and 83, 95, 59, and 98%, respectively, for M. kansasii. With training and experience, these results improved to 74.5, 98, 96, and 84% and 93, 98, 79, and 98%, respectively. The major improvements were in distinguishing the pseudocording, or loose aggregation ofMycobacterium avium complex from M. tuberculosis and the long beaded forms of Mycobacterium gordonae from M. kansasii. Mycobacterium asiaticum and Mycobacterium szulgai, which rarely occur, are genetically related to M. kansasiiand morphologically difficult to distinguish. In defined circumstances, serpentine cording and cross-barring can be used for rapid presumptive identification of M. tuberculosisand M. kansasii, respectively, and as guides for initial probe selection to reduce costs.

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