Clinical Evaluation of a Commercial Ligase-Based Gene Amplification Method for Detection of Mycobacterium tuberculosis

1998 ◽  
Vol 17 (6) ◽  
pp. 371-376
Author(s):  
P. Alonso ◽  
A. Orduña ◽  
M. A. Bratos ◽  
A. San Miguel ◽  
A. Rodríguez Torres
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Gene Amplification ◽  
Clinical Evaluation ◽  
Amplification Method

scholarly journals Study on cartridge based nucleic acid amplification test in children with Neurotuberculosis at RNT Medical College, Udaipur, Rajasthan, India

2019 ◽  
Vol 6 (4) ◽  
pp. 1588
Author(s):  
Shashi Bala ◽  
Suresh Goyal
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Clinical Evaluation ◽  
Controlled Trial ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Gastric Aspirate ◽  
Age Group ◽  
Major Health Problem ◽  
Medical College

Background: The aim was to determine utility of Cartridge based nucleic acid amplification test (CBNAAT) in diagnosis of mycobacterium tuberculosis in children with neurotuberculosis diagnosed on the basis of clinical evaluation, CSF findings and neuroimaging.Methods: A prospective randomized controlled trial was conducted in Pediatric Department of RNT Medical College, Udaipur, Rajasthan, India from July 2017 to June 2018. Total 110 children of age group of 6 months to 18 years with the diagnosis of tubercular meningoencephalitis (TBME) on the basis of clinical evaluation, CSF examination and neuroimaging were included in the study.Results: A total 110 children were enrolled. Maximum number of cases admitted with TBME were among 1-5 years of age group (60.91%). CSF and gastric aspirate were examined by CBNAAT for MTB. 5 (4.55%) children had CBNAAT positivity in CSF. Gastric aspirate was positive among 16 (14.55%) children. None of the patient had CBNAAT positive result both in CSF and gastric aspirate.Conclusions: TBME is a major health problem in children below 5 years. Gene Xpert assay has the potential to significantly improve and escalate the diagnosis of smear-negative body fluid specimens. CBNAAT for mycobacterium tuberculosis was positive in 5 (4.55%) children from CSF and 16 (14.55%) from gastric aspirate. Negative CBNAAT should not prevent any patient with suspected features of TBME from starting anti tubercular treatment (ATT) as sensitivity of this test remains low. Final judgement to start ATT should be based on clinical, biochemical and radiological profile especially in CNS tuberculosis.

Loop-mediated isothermal amplification technique: principle, development and wide application in food safety

2020 ◽  
Vol 12 (46) ◽  
pp. 5551-5561
Author(s):  
Tianzeng Huang ◽  
Linzhi Li ◽  
Xing Liu ◽  
Qi Chen ◽  
Xueen Fang ◽  
...  
Keyword(s):  
Food Safety ◽  
Gene Amplification ◽  
Foodborne Pathogens ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Isothermal Conditions ◽  
Loop Mediated Isothermal Amplification ◽  
Novel Gene ◽  
Amplification Method ◽  
Amplification Technique

LAMP is a relatively novel gene amplification method under isothermal conditions with rapidity, and high specificity. It is widely applied in the field of food safety, such as in the detection of foodborne pathogens, GM, OP pesticides and so on

Rapid detection of pathogenic virus genome sequence from throat and nasal swab samples using an exhaustive gene amplification method

2019 ◽  
Vol 15 (3) ◽  
pp. 399-403 ◽  
Author(s):  
Junji Hosokawa-Muto ◽  
Hiroki Sakai ◽  
Yukiko Sassa ◽  
Yoshihito Fujinami ◽  
Mai Kishimoto ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Genome Sequence ◽  
Rapid Detection ◽  
Nasal Swab ◽  
Virus Genome ◽  
Amplification Method ◽  
Pathogenic Virus

Investigation of the Molecular Mechanism of ICAN, a Novel Gene Amplification Method

2007 ◽  
Vol 142 (2) ◽  
pp. 283-292 ◽  
Author(s):  
T. Uemori ◽  
H. Mukai ◽  
O. Takeda ◽  
M. Moriyama ◽  
Y. Sato ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Gene Amplification ◽  
Novel Gene ◽  
Amplification Method

High levels of human recombinant cyclooxygenase-1 expression in mammalian cells using a novel gene amplification method

2011 ◽  
Vol 80 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Hiromitsu Yoshimura ◽  
Shingo Sekine ◽  
Hisashi Adachi ◽  
Yoshikatsu Uematsu ◽  
Akiko Mitani ◽  
...  
Keyword(s):  
Gene Amplification ◽  
Mammalian Cells ◽  
Novel Gene ◽  
Amplification Method ◽  
Cyclooxygenase 1 ◽  
Recombinant Cyclooxygenase 1 ◽  
Expression In Mammalian Cells
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