Evaluation of a Commercial Probe Assay for Detection of Rifampin Resistance in Mycobacterium tuberculosis Directly from Respiratory and Nonrespiratory Clinical Samples

1998 ◽  
Vol 17 (3) ◽  
pp. 189-192
Author(s):  
F. Gamboa ◽  
P. J. Cardona ◽  
J. M. Manterola ◽  
J. Lonca ◽  
L. Matas ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Samples ◽  
Rifampin Resistance ◽  
Probe Assay

AID TB resistance line probe assay for rapid detection of resistant Mycobacterium tuberculosis in clinical samples

2015 ◽  
Vol 70 (4) ◽  
pp. 400-408 ◽  
Author(s):  
B. Molina-Moya ◽  
A. Lacoma ◽  
C. Prat ◽  
J. Diaz ◽  
A. Dudnyk ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Rapid Detection ◽  
Clinical Samples ◽  
Line Probe Assay ◽  
Probe Assay

scholarly journals Detection of Rifampin Resistance in Mycobacterium tuberculosis by Double Gradient-Denaturing Gradient Gel Electrophoresis

1999 ◽  
Vol 43 (10) ◽  
pp. 2550-2554 ◽  
Author(s):  
Paolo Scarpellini ◽  
Sergio Braglia ◽  
Paola Carrera ◽  
Maura Cedri ◽  
Paola Cichero ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Gel Electrophoresis ◽  
Rapid Detection ◽  
Susceptibility Testing ◽  
Denaturing Gradient Gel Electrophoresis ◽  
The Other ◽  
Clinical Samples ◽  
Pcr Products ◽  
Gradient Gel Electrophoresis ◽  
Rifampin Resistance

ABSTRACT We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance fromrpoB PCR products of Mycobacterium tuberculosisisolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mutations for predicting RMP resistance.

scholarly journals Comparison of Three Molecular Assays for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis

1998 ◽  
Vol 36 (7) ◽  
pp. 1969-1973 ◽  
Author(s):  
Simon A. Watterson ◽  
Stuart M. Wilson ◽  
Malcolm D. Yates ◽  
Francis A. Drobniewski
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Surrogate Marker ◽  
Multidrug Resistant ◽  
Diagnostic Methods ◽  
Ratio Method ◽  
Molecular Assays ◽  
Mdr Tb ◽  
Rifampin Resistance ◽  
Probe Assay ◽  
Susceptibility Tests

Multidrug-resistant Mycobacterium tuberculosis (MDR-TB) is an emerging problem of great importance to public health, with higher mortality rates than drug-sensitive TB, particularly in immunocompromised patients. MDR-TB patients require treatment with more-toxic second-line drugs and remain infectious for longer than patients infected with drug-sensitive strains, incurring higher costs due to prolonged hospitalization. It is estimated that 90% of United Kingdom rifampin-resistant isolates are also resistant to isoniazid, making rifampin resistance a useful surrogate marker for multidrug resistance and indicating that second- and third-line drugs to which these isolates are susceptible are urgently required. Resistance in approximately 95% of rifampin-resistant isolates is due to mutations in a 69-bp region of the rpoB gene, making this a good target for molecular genotypic diagnostic methods. Two molecular assays, INNO-LiPA Rif.TB (Innogenetics, Zwijndrecht, Belgium) and MisMatch Detect II (Ambion, Austin, Tex.), were performed on primary specimens and cultures to predict rifampin resistance, and these methods were compared with the resistance ratio method. A third method, the phenotypic PhaB assay, was also evaluated in comparison to cultures in parallel with the genotypic assays. In an initial evaluation 16 of 16, 15 of 16, and 16 of 16 rifampin-resistant cultures (100, 93.8, and 100%, respectively), were correctly identified by line probe assay (LiPA), mismatch assay, and PhaB assay, respectively. Subsequently 38 sputa and bronchealveolar lavage specimens and 21 isolates were received from clinicians for molecular analysis. For the 38 primary specimens the LiPA and mismatch assay correlated with culture and subsequent identification and susceptibility tests in 36 and 38 specimens (94.7 and 100%), respectively. For the 21 isolates submitted by clinicians, both assays correlated 100% with routine testing.

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