Comparative Study between Line Probe Assay by MTBDRplus (Hain PCR) and XpertMTB/RIF Assay (GeneXpert PCR) for the Detection of Mycobacterium Tuberculosis Complex Directly from Clinical Samples and from Cultured Isolates

2016 ◽  
Vol 25 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Dalia El-Hossary
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Comparative Study ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Samples ◽  
Line Probe Assay ◽  
Tuberculosis Complex ◽  
Probe Assay

scholarly journals Line probe assay for detection of Mycobacterium tuberculosis complex: An experience from Central India

2017 ◽  
Vol 145 (1) ◽  
pp. 70 ◽  
Author(s):  
Prabha Desikan ◽  
Nikita Panwalkar ◽  
ShainaBeg Mirza ◽  
Aparna Chaturvedi ◽  
Kudsia Ansari ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Central India ◽  
Line Probe Assay ◽  
Tuberculosis Complex ◽  
Probe Assay

scholarly journals A study on mutation in genes associated with rifampicin and isoniazid resistance in Mycobacterium tuberculosis complex using line probe assay

2018 ◽  
Vol 6 (12) ◽  
pp. 3997
Author(s):  
Rohit Kumar ◽  
Shivendra Kumar Shahi ◽  
Rakesh Kumar ◽  
Balkrishna Mishra ◽  
Shailesh Kumar ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Diagnosis ◽  
Pulmonary Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Smear Microscopy ◽  
Common Mutation ◽  
Line Probe Assay ◽  
Tuberculosis Complex ◽  
Sensitivity Pattern ◽  
Probe Assay

Background: The term tuberculosis describe a clinical illness, which is predominantly caused by Mycobacterium tuberculosis and less common by other species. Infection is transmitted by infected droplets through respiratory route. Early diagnosis and appropriate management is the only way to control the spread of infection. The available diagnostic tools include, smear microscopy, culture and molecular methods. Culture is the gold standard, but it takes around 2-8 weeks to get the result and smear microscopy having less sensitivity. Molecular technique especially Line probe assay can be better option because of high sensitivity and specificity, and directly clinical sample can be used, and result will be made available within same day with sensitivity pattern. Present study was designed to use of LPA for early diagnosis.Methods: Laboratory based observational study conducted in department of microbiology, IGIMS Patna and TBDC, Patna. Sputum specimens were collected from clinically suspected cases of pulmonary tuberculosis, and subjected to smear microscopy, culture and LPA.Results: During the study period, 2841 patients were diagnosed as pulmonary tuberculosis. Strain of Mycobacterium tuberculosis complex in, 12% (347) patients were rifampicin and isoniazid resistant, 4% (117) and 3% (86) patients were rifampicin and isoniazid mono-resistant respectively. We found that rpoB MUT3 was the most common mutation in gene associated with rifampicin resistant and katG MUT1gene associated with isoniazid resistant.Conclusions: Present study support the use of LPA for early diagnosis of smear positive as well as smear negative pulmonary tuberculosis cases. Resulting early diagnosis and appropriate management of patients.

Sign in / Sign up

Export Citation Format

Share Document