Nucleoside diphosphate kinase in fetal rat bone tissue: increased expression in osteoblasts and localization in the matrix vesicle fraction

1998 ◽  
Vol 16 (4) ◽  
pp. 215-226 ◽  
Author(s):  
Yoshiro Yasutomo ◽  
Naoshi Ishikawa ◽  
Naokazu Nagata ◽  
Narimichi Kimura
Keyword(s):  
Bone Tissue ◽  
Nucleoside Diphosphate Kinase ◽  
Matrix Vesicle ◽  
Fetal Rat ◽  
The Matrix ◽  
Rat Bone

scholarly journals Histomorphometric identification of carbonic anhydrase in fetal rat bone embedded in glycolmethacrylate.

1987 ◽  
Vol 35 (2) ◽  
pp. 245-250 ◽  
Author(s):  
P J Marie ◽  
M Hott
Keyword(s):  
Acid Phosphatase ◽  
Carbonic Anhydrase ◽  
Bone Tissue ◽  
Specific Inhibitor ◽  
Slight Modification ◽  
Histochemical Staining ◽  
Histochemical Reaction ◽  
Low Concentrations ◽  
Fetal Rat ◽  
Rat Bone

Carbonic anhydrase was identified in bone-resorbing cells present in sections of fetal rat femur embedded in glycolmethacrylate. Using a slight modification of the Hansson's histochemical method, we demonstrated that most chondroclasts (91.8-95.4%) and osteoclasts (95.1-96.3%) display a positive histochemical reaction for carbonic anhydrase. This staining was consistently inhibited in the presence of very low concentrations (10(-6), 10(-7) M) of the specific inhibitor acetazolamide. The number of chondroclasts reacting for carbonic anhydrase was identical to the number of acid phosphatase-stained chondroclasts determined on adjacent sections. A large majority of osteoclasts (96.3%) stained for carbonic anhydrase and for acid phosphatase (97.2%), with more osteoclasts reacting for the latter enzyme than the former (76.8 +/- 8.5 (SD) vs 85.3 +/- 9.2 cells/mm2 of endosteal bone; p less than 0.01). The observation that acetazolamide at a concentration as low as 10(-7) M inhibited Hansson's reaction, together with our histomorphometric results, validates the use of histochemical staining for carbonic anhydrase to evaluate activity of bone-resorbing cells identified in plastic-embedded fetal bone tissue.

Proteoglycan synthesis and deposition in fetal rat bone

Biochemistry ◽  
1984 ◽  
Vol 23 (7) ◽  
pp. 1572-1576 ◽  
Author(s):  
Graeme K. Hunter ◽  
Johan N. M. Heersche ◽  
Jane E. Aubin
Keyword(s):  
Proteoglycan Synthesis ◽  
Fetal Rat ◽  
Rat Bone

Thyroid hormones increase insulin-like growth factor I content in the medium of rat bone tissue

2009 ◽  
Vol 8 (12) ◽  
pp. 1475-1481 ◽  
Author(s):  
Peter Lakatos ◽  
Matthew D. Caplice ◽  
Vikram Khanna ◽  
Paula H. Stern
Keyword(s):  
Growth Factor ◽  
Thyroid Hormones ◽  
Bone Tissue ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Growth Factor I ◽  
Rat Bone

Activation of serum complement inhibits collagen synthesis in fetal rat bone in organ culture

1982 ◽  
Vol 34 (1) ◽  
pp. 370-375 ◽  
Author(s):  
Barbara E. Kream ◽  
Lawrence G. Raisz ◽  
Ann L. Sandberg
Keyword(s):  
Organ Culture ◽  
Collagen Synthesis ◽  
Serum Complement ◽  
Fetal Rat ◽  
Rat Bone

Biological Response to Wollastonite Doped α-Tricalcium Phosphate Implants in Hard and Soft Tissues in Rats

2008 ◽  
Vol 396-398 ◽  
pp. 7-10 ◽  
Author(s):  
Ana Maria Minarelli Gaspar ◽  
Sybele Saska ◽  
R. García Carrodeguas ◽  
A.H. De Aza ◽  
P. Pena ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Bone Formation ◽  
Bone Tissue ◽  
Tricalcium Phosphate ◽  
Rattus Norvegicus ◽  
Soft Tissues ◽  
Subcutaneous Tissue ◽  
Biological Response ◽  
New Bone Formation ◽  
Rat Bone

The biological response following subcutaneous and bone implantation of β-wollastonite(β-W)-doped α-tricalcium phosphate bioceramics in rats was evaluated. Tested materials were: tricalcium phosphate (TCP), consisting of a mixture of α- and β-polymorphs; TCP doped with 5 wt. % of β-W (TCP5W), composed of α-TCP as only crystalline phase; and TCP doped with 15 wt. % of β-W (TCP15), containing crystalline α-TCP and β-W. Cylinders of 2x1 mm were implanted in tibiae and backs of adult male Rattus norvegicus, Holtzman rats. After 7, 30 and 120 days, animals were sacrificed and the tissue blocks containing the implants were excised, fixed and processed for histological examination. TCP, TCP5W and TCP15W implants were biocompatible but neither bioactive nor biodegradable in rat subcutaneous tissue. They were not osteoinductive in connective tissue either. However, in rat bone tissue β-W-doped α-TCP implants (TCP5W and TCP15W) were bioactive, biodegradable and osteoconductive. The rates of biodegradation and new bone formation observed for TCP5W and TCP15W implants in rat bone tissue were greater than for non-doped TCP.

scholarly journals CHONDROGENESIS, STUDIED WITH THE ELECTRON MICROSCOPE

1960 ◽  
Vol 8 (3) ◽  
pp. 719-760 ◽  
Author(s):  
Gabriel C. Godman ◽  
Keith R. Porter
Keyword(s):  
Golgi Apparatus ◽  
Ground Substance ◽  
Cell Cortex ◽  
Matrix Space ◽  
Cytoplasmic Inclusions ◽  
Structural Modifications ◽  
Fetal Rat ◽  
The Matrix ◽  
Connective Tissue Matrix ◽  
Tissue Matrix

The role of the cells in the fabrication of a connective tissue matrix, and the structural modifications which accompany cytodifferentiation have been investigated in developing epiphyseal cartilage of fetal rat by means of electron microscopy. Differentiation of the prechondral mesenchymal cells to chondroblasts is marked by the acquisition of an extensive endoplasmic reticulum, enlargement and concentration of the Golgi apparatus, the appearance of membrane-bounded cytoplasmic inclusions, and the formation of specialized foci of increased density in the cell cortex. These modifications are related to the secretion of the cartilage matrix. The matrix of young hyaline cartilage consists of groups of relatively short, straight, banded collagen fibrils of 10 to 20 mµ and a dense granular component embedded in an amorphous ground substance of moderate electron density. It is postulated that the first phase of fibrillogenesis takes place at the cell cortex in dense bands or striae within the ectoplasm subjacent to the cell membrane. These can be resolved into sheaves of "primary" fibrils of about 7 to 10 mµ. They are supposedly shed (by excortication) into the matrix space between the separating chondroblasts, where they may serve as "cores" of the definitive matrix fibrils. The diameter of the fibrils may subsequently increase up to threefold, presumably by incorporation of "soluble" or tropocollagen units from the ground substance. The chondroblast also discharges into the matrix the electrondense amorphous or granular contents of vesicles derived from the Golgi apparatus, and the mixed contents of large vacuoles or blebs bounded by distinctive double membranes. Small vesicles with amorphous homogeneous contents of moderate density are expelled in toto from the chondroblasts. In their subsequent evolution to chondrocytes, both nucleus and cytoplasm of the chondroblasts undergo striking condensation. Those moving toward the osteogenic plate accumulate increasingly large stores of glycogen. In the chondrocyte, the enlarged fused Golgi vesicles with dense contents, massed in the juxtanuclear zone, are the most prominent feature of the cytoplasm. Many of these make their way to the surface to discharge their contents. The hypertrophied chondrocytes of the epiphyseal plate ultimately yield up their entire contents to the matrix.

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