Progesterone-mediated stimulation of osteoprogenitor proliferation and differentiation in cell populations derived from adult or fetal rat bone tissue depends on the serum component of the culture media

1997 ◽  
Vol 7 (4) ◽  
pp. 323-330 ◽  
Author(s):  
Y. Ishida ◽  
C. G. Bellows ◽  
I. Tertinegg ◽  
J. N. M. Heersche
Keyword(s):  
Bone Tissue ◽  
Culture Media ◽  
Cell Populations ◽  
Fetal Rat ◽  
Serum Component ◽  
Proliferation And Differentiation ◽  
Rat Bone ◽  
Stimulation Of

scholarly journals Intestinal crypt derived enteroid coculture in presence of peristaltic longitudinal muscle myenteric plexus

2020 ◽  
Author(s):  
Daniel E Levin ◽  
Arabinda Mandal ◽  
Mark A Fleming ◽  
Katherine H Bae ◽  
Brielle Gerry ◽  
...  
Keyword(s):  
Myenteric Plexus ◽  
Longitudinal Muscle ◽  
Culture Media ◽  
Ex Vivo ◽  
Enteric Neurons ◽  
Intestinal Cell ◽  
Cell Populations ◽  
Complex Interactions ◽  
Proliferation And Differentiation ◽  
Intestinal Peristalsis

Abstract The role of enteric neurons in driving intestinal peristalsis has been known for over a century. However, in recent decades, scientists have begun to unravel additional complex interactions between this nerve plexus and other cell populations in the intestine. Investigations into these potential interactions is complicated by a paucity of tractable models of these cellular relationships. Here, we describe a novel technique for ex vivo coculture of enteroids, so called “mini-guts,” in juxtaposition to the longitudinal muscle myenteric plexus (LMMP). Key to this system, we developed a LMMP culture media that: 1) allows the LMMP to maintain ex vivo peristalsis for 2 weeks along with proliferation of neurons, glia, smooth muscle and fibroblast cells, and 2) supports the proliferation and differentiation of the intestinal stem cells into enteroids complete with epithelial enterocytes, Paneth cells, goblet cells and enteroendocrine cells. Importantly, this technique identifies a culture condition that supports both the metabolic needs of intestinal epithelium as well as neuronal elements, demonstrating the feasibility of maintaining these two populations in a single culture system. This sets the stage for experiments to better define the regulatory interactions of these two important intestinal cell populations.

scholarly journals Histomorphometric identification of carbonic anhydrase in fetal rat bone embedded in glycolmethacrylate.

1987 ◽  
Vol 35 (2) ◽  
pp. 245-250 ◽  
Author(s):  
P J Marie ◽  
M Hott
Keyword(s):  
Acid Phosphatase ◽  
Carbonic Anhydrase ◽  
Bone Tissue ◽  
Specific Inhibitor ◽  
Slight Modification ◽  
Histochemical Staining ◽  
Histochemical Reaction ◽  
Low Concentrations ◽  
Fetal Rat ◽  
Rat Bone

Carbonic anhydrase was identified in bone-resorbing cells present in sections of fetal rat femur embedded in glycolmethacrylate. Using a slight modification of the Hansson's histochemical method, we demonstrated that most chondroclasts (91.8-95.4%) and osteoclasts (95.1-96.3%) display a positive histochemical reaction for carbonic anhydrase. This staining was consistently inhibited in the presence of very low concentrations (10(-6), 10(-7) M) of the specific inhibitor acetazolamide. The number of chondroclasts reacting for carbonic anhydrase was identical to the number of acid phosphatase-stained chondroclasts determined on adjacent sections. A large majority of osteoclasts (96.3%) stained for carbonic anhydrase and for acid phosphatase (97.2%), with more osteoclasts reacting for the latter enzyme than the former (76.8 +/- 8.5 (SD) vs 85.3 +/- 9.2 cells/mm2 of endosteal bone; p less than 0.01). The observation that acetazolamide at a concentration as low as 10(-7) M inhibited Hansson's reaction, together with our histomorphometric results, validates the use of histochemical staining for carbonic anhydrase to evaluate activity of bone-resorbing cells identified in plastic-embedded fetal bone tissue.

Differential actions of prostaglandins in separate cell populations from fetal rat bone.

Endocrinology ◽  
1994 ◽  
Vol 135 (4) ◽  
pp. 1611-1620 ◽  
Author(s):  
M Centrella ◽  
S Casinghino ◽  
T L McCarthy
Keyword(s):  
Cell Populations ◽  
Separate Cell ◽  
Fetal Rat ◽  
Rat Bone

scholarly journals Differential effects of transforming growth factor-beta on the synthesis of extracellular matrix proteins by normal fetal rat calvarial bone cell populations.

1988 ◽  
Vol 106 (3) ◽  
pp. 915-924 ◽  
Author(s):  
J L Wrana ◽  
M Maeno ◽  
B Hawrylyshyn ◽  
K L Yao ◽  
C Domenicucci ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Plasminogen Activator Inhibitor ◽  
Bone Cell ◽  
Cell Populations ◽  
Tgf Beta ◽  
Differential Effects ◽  
Fetal Rat ◽  
Activator Inhibitor ◽  
Stimulation Of

To determine the effects of transforming growth factor-beta (TGF-beta) on the different cell types that exist in bone, cell populations (I-IV), progressively enriched in osteoblastic cells relative to fibroblastic cells, were prepared from fetal rat calvaria using timed collagenase digestions. TGF-beta did not induce anchorage-independent growth of these cells, nor was anchorage-dependent growth stimulated in most populations studied, despite a two- to threefold increase in the synthesis of cellular proteins. In all populations the synthesis of secreted proteins increased 2-3.5-fold. In particular, collagen, fibronectin, and plasminogen activator inhibitor synthesis was stimulated. However, different degrees of stimulation of individual proteins were observed both within and between cell populations. A marked preferential stimulation of plasminogen activator inhibitor was observed in each population, together with a slight preferential stimulation of collagen; the effect on collagen expression being directed primarily at type I collagen. In contrast, the synthesis of SPARC (secreted protein acidic rich in cysteine/osteonectin was stimulated approximately two-fold by TGF-beta, but only in fibroblastic populations. Collectively, these results demonstrate that TGF-beta stimulates matrix production by bone cells and, through differential effects on individual matrix components, may also influence the nature of the matrix formed by different bone cell populations. In the presence of TGF-beta, osteoblastic cells lost their polygonal morphology and alkaline phosphatase activity was decreased, reflecting a suppression of osteoblastic features. The differential effects of TGF-beta on bone cell populations are likely to be important in bone remodeling and fracture repair.

scholarly journals Nanoscale Strontium-Substituted Hydroxyapatite Pastes and Gels for Bone Tissue Regeneration

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1611
Author(s):  
Caroline J. Harrison ◽  
Paul V. Hatton ◽  
Piergiorgio Gentile ◽  
Cheryl A. Miller
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Culture Media ◽  
Full Range ◽  
Sol Gel ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Tissue Culture Media ◽  
Bone Deposition

Injectable nanoscale hydroxyapatite (nHA) systems are highly promising biomaterials to address clinical needs in bone tissue regeneration, due to their excellent biocompatibility, bioinspired nature, and ability to be delivered in a minimally invasive manner. Bulk strontium-substituted hydroxyapatite (SrHA) is reported to encourage bone tissue growth by stimulating bone deposition and reducing bone resorption, but there are no detailed reports describing the preparation of a systematic substitution up to 100% at the nanoscale. The aim of this work was therefore to fabricate systematic series (0–100 atomic% Sr) of SrHA pastes and gels using two different rapid-mixing methodological approaches, wet precipitation and sol-gel. The full range of nanoscale SrHA materials were successfully prepared using both methods, with a measured substitution very close to the calculated amounts. As anticipated, the SrHA samples showed increased radiopacity, a beneficial property to aid in vivo or clinical monitoring of the material in situ over time. For indirect methods, the greatest cell viabilities were observed for the 100% substituted SrHA paste and gel, while direct viability results were most likely influenced by material disaggregation in the tissue culture media. It was concluded that nanoscale SrHAs were superior biomaterials for applications in bone surgery, due to increased radiopacity and improved biocompatibility.

Proteoglycan synthesis and deposition in fetal rat bone

Biochemistry ◽  
1984 ◽  
Vol 23 (7) ◽  
pp. 1572-1576 ◽  
Author(s):  
Graeme K. Hunter ◽  
Johan N. M. Heersche ◽  
Jane E. Aubin
Keyword(s):  
Proteoglycan Synthesis ◽  
Fetal Rat ◽  
Rat Bone
Sign in / Sign up

Export Citation Format

Share Document