Serological and coat protein sequence studies suggest that necrosis disease on sunflower in India is caused by a strain of Tobacco streak ilarvirus

2002 ◽  
Vol 147 (3) ◽  
pp. 651-658 ◽  
Author(s):  
A. I. Bhat ◽  
R. K. Jain ◽  
A. Kumar ◽  
M. Ramiah ◽  
A. Varma
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Coat Protein Sequence

scholarly journals Requirements for the Packaging of Geminivirus Circular Single-Stranded DNA: Effect of DNA Length and Coat Protein Sequence

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1235
Author(s):  
Keith Saunders ◽  
Jake Richardson ◽  
David M. Lawson ◽  
George P. Lomonossoff
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Expression System ◽  
Coat Protein Sequence ◽  
Full Length ◽  
Plant Leaves ◽  
Single Stranded Dna ◽  
Half Length

Geminivirus particles, consisting of a pair of twinned isometric structures, have one of the most distinctive capsids in the virological world. Until recently, there was little information as to how these structures are generated. To address this, we developed a system to produce capsid structures following the delivery of geminivirus coat protein and replicating circular single-stranded DNA (cssDNA) by the infiltration of gene constructs into plant leaves. The transencapsidation of cssDNA of the Begomovirus genus by coat protein of different geminivirus genera was shown to occur with full-length but not half-length molecules. Double capsid structures, distinct from geminate capsid structures, were also generated in this expression system. By increasing the length of the encapsidated cssDNA, triple geminate capsid structures, consisting of straight, bent and condensed forms were generated. The straight geminate triple structures generated were similar in morphology to those recorded for a potato-infecting virus from Peru. These finding demonstrate that the length of encapsidated DNA controls both the size and stability of geminivirus particles.

Beet luteovirus coat protein sequence variation

1995 ◽  
Vol 127 (1) ◽  
pp. 113-124 ◽  
Author(s):  
J. R. MIRANDA ◽  
M. STEVENS ◽  
E. BRUYNE ◽  
H. G. SMITH ◽  
C. BIRD ◽  
...  
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Sequence Variation ◽  
Coat Protein Sequence

scholarly journals Preliminary molecular characterization of some Citrus tristeza Closterovirus isolates infecting Croatian citrus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 264-266
Author(s):  
S. Černi ◽  
D. Škorić ◽  
M. Krajačić
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Coastal Region ◽  
Coat Protein Sequence ◽  
Rt Pcr ◽  
Double Stranded Rna ◽  
The World ◽  
Citrus Tristeza Closterovirus ◽  
Citrus Tristeza

Citrus tristeza Closterovirus (CTV) is widespread in major citrus-growing regions of the world often causing destructive diseases. Citrus samples were taken from orchards in the Croatian coastal region. CTV was detected in two symptomless field trees of Satsuma mandarins and one diseased lemon tree. Double-stranded RNA was isolated from the field trees and the dsRNA patterns were compared in polyacrylamide gels. The same dsRNA extracts were used as templates in RT-PCR experiments amplifying the CTV coat protein sequence. Amplicons were subjected to SSCP and RFLP analyses. The results indicate greater similarity between CTV isolates from Satsuma mandarins than between these two and the lemon isolate.

Sign in / Sign up

Export Citation Format

Share Document