scholarly journals Preliminary molecular characterization of some Citrus tristeza Closterovirus isolates infecting Croatian citrus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 264-266
Author(s):  
S. Černi ◽  
D. Škorić ◽  
M. Krajačić
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Coastal Region ◽  
Coat Protein Sequence ◽  
Rt Pcr ◽  
Double Stranded Rna ◽  
The World ◽  
Citrus Tristeza Closterovirus ◽  
Citrus Tristeza

Citrus tristeza Closterovirus (CTV) is widespread in major citrus-growing regions of the world often causing destructive diseases. Citrus samples were taken from orchards in the Croatian coastal region. CTV was detected in two symptomless field trees of Satsuma mandarins and one diseased lemon tree. Double-stranded RNA was isolated from the field trees and the dsRNA patterns were compared in polyacrylamide gels. The same dsRNA extracts were used as templates in RT-PCR experiments amplifying the CTV coat protein sequence. Amplicons were subjected to SSCP and RFLP analyses. The results indicate greater similarity between CTV isolates from Satsuma mandarins than between these two and the lemon isolate.

scholarly journals The molecular characterization of the coat protein sequence and differentiation of CMV- subgroup I on tobacco from native flora in Turkey

2020 ◽  
Vol 48 (2) ◽  
pp. 523-534
Author(s):  
Mustafa USTA ◽  
Abdullah GÜLLER ◽  
Abidin GÜNAY
Keyword(s):  
Coat Protein ◽  
Sequence Similarity ◽  
Coat Protein Sequence ◽  
Rt Pcr ◽  
Plant Host ◽  
Native Flora ◽  
Subgroup Ii ◽  
Escherichia Coli Jm109 ◽  
First Time

Cucumber mosaic virus (CMV) has a broad plant-host range and a wide ecological zone distribution. Virus-like symptoms were observed on tobacco fields of Adiyaman province (Turkey) showing conspicuous mottling, greenish mosaic patterns and severe malformations of leaves. A total of forty tobacco samples tested positive against CMV by reverse transcription polymerase chain reaction (RT-PCR) using coat protein gene specific primers. Five randomly chosen CMV isolates were cloned into pGEM T-Easy vector and transformed into Escherichia coli JM109 strain. The recombinant bacterial clones containing insert-DNA were further purified and sequenced bidirectionally. In multiplex-RT-PCR studies carried out, it was found that all 40 CMV isolates belong to Subgroup I by resulting a 593 bp long DNA fragments. CMV subgroup IA was found to predominate in 4 out of 5 tobacco samples and CMV subgroup IB was found in 1 out of 5 CMV-positive samples by comparing the isolates with CMV reference isolates in phylogenetic tree. However, no Subgroup II sequences were found by multiplex RT-PCR using discriminating primers. The nucleic acid sequences were analyzed for the investigation of diversity of coat protein (CP) sequences of 5 CMV isolates. The sequence similarity ranged from 94.2-100% with the CMV subgroup I isolates infecting diverse plants in other regions of the world. The evolutionary tree revealed that the CMV IA Adiyaman isolates exhibited a genetic affinity with Australian and Spanish isolates. However, the CMV IB Adiyaman isolate showed a close genetic relationship with only the Australian isolates. To our knowledge, this study shows for the first time the occurrence of CMV IA and IB isolates infecting cultured tobacco plants in Adiyaman province.

scholarly journals Biological and Molecular Characterization of a Novel Tobamovirus with a Unique Host Range

Plant Disease ◽  
2003 ◽  
Vol 87 (10) ◽  
pp. 1190-1196 ◽  
Author(s):  
Scott Adkins ◽  
Ivanka Kamenova ◽  
Diann Achor ◽  
Dennis J. Lewandowski
Keyword(s):  
Coat Protein ◽  
Host Range ◽  
Protein Sequence ◽  
Genome Organization ◽  
Protein Gene ◽  
Coat Protein Sequence ◽  
Plant Viruses ◽  
Experimental Host Range ◽  
Solanaceous Plants

Tobamoviruses are among the best characterized and most studied plant viruses. Three subgroups of tobamoviruses correspond to viral genome sequence and host range to include those viruses infecting (i) solanaceous plants, (ii) brassicas, or (iii) cucurbits or legumes. We isolated a virus from Florida landscape plantings of the malvaceous plant hibiscus (Hibiscus rosasinensis) that appears to be a tobamovirus based upon its virion morphology, genome organization, and coat protein sequence. The experimental host range of this virus included five malvaceous species but excluded all tested brassica, cucurbit, and legume species and 12 of the 19 solanaceous species tested. The unique host range and comparison of coat protein gene and protein sequences with those of recognized tobamoviruses indicate that this is a novel to-bamovirus. A limited survey revealed that this virus is widespread in hibiscus and related species in the Florida landscape.

Differentiation of Allium carlaviruses isolated from different parts of the world based on the viral coat protein sequence

1998 ◽  
Vol 143 (6) ◽  
pp. 1093-1107 ◽  
Author(s):  
T. Tsuneyoshi ◽  
T. Matsumi ◽  
T. C. Deng ◽  
I. Sako ◽  
S. Sumi
Keyword(s):  
Coat Protein ◽  
Protein Sequence ◽  
Coat Protein Sequence ◽  
Viral Coat Protein ◽  
The World ◽  
Viral Coat ◽  
Different Parts

Serological Characterization of Hungarian Plum Pox Virus Isolates

1997 ◽  
Vol 52 (5-6) ◽  
pp. 391-395
Author(s):  
Juan José López-Moya ◽  
Dionisio López-Abella ◽  
José-Ramón Díaz-Rúiz ◽  
Belén Martinez-Garcia ◽  
Richard Gáborjányi
Keyword(s):  
Monoclonal Antibodies ◽  
Coat Protein ◽  
Blot Analysis ◽  
Plum Pox Virus ◽  
Rt Pcr ◽  
Dot Blot ◽  
Serological Characterization ◽  
Spanish Isolate ◽  
Virus Isolates

Abstract Three Hungarian (No.2, 4 and 9), and a Moldavian (K) plum pox virus isolates were compared with a characterized Spanish isolate (5.15) by RT-PCR, ELISA, dot-blot and West­ern blot analysis. Monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the Spanish isolate were able to differentiate the four isolates. Hungarian isolate No. 2 proved to be serologically identical to the Spanish isolate, while No. 4 showed appreciable differences and No. 9 could be recognized only by the monoclonal antibodies representing the intermedial and internal parts of the coat protein. K isolate showed a more distant relationship to other isolates. Our experiment provided the first demonstration of the presence of D type isolates in Hungary.

Molecular detection and coat protein gene based characterization of Citrus tristeza virus prevalent in Sikkim state of India

2019 ◽  
Vol 73 (1) ◽  
pp. 135-143 ◽  
Author(s):  
Ashish Warghane ◽  
Amol Kokane ◽  
Sunil Kokane ◽  
Manali Motghare ◽  
Datta Surwase ◽  
...  
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Molecular Detection ◽  
Citrus Tristeza Virus ◽  
Protein Gene ◽  
Tristeza Virus ◽  
Citrus Tristeza

scholarly journals The Whole Genome Sequence of a Novel Chrysovirus From Beauveria Bassiana Vuillemin, an Entomopathogenic Fungus

2021 ◽  
Author(s):  
Le Li ◽  
Qin Kang ◽  
Song-Bai Zhang ◽  
Du Hai ◽  
Yang Lu ◽  
...  
Keyword(s):  
Amino Acid ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungus ◽  
Whole Genome Sequence ◽  
Metagenomic Sequencing ◽  
Rt Pcr ◽  
Double Stranded Rna ◽  
Dsrna Mycovirus ◽  
Arthropod Pest

Abstract Beauveria bassiana, an entomopathogenic fungus, has a wide host range and is used for arthropod pest control worldwide. Here, we report the discovery and characterization of a novel double-stranded RNA (dsRNA) mycovirus Beauveria bassiana chrysovirus 2 (BbCV2), isolated from the B. bassiana from China. The genome of the virus was determined by metagenomic sequencing, RT-PCR, and RACE cloning comprises four dsRNA segments that are 3441bp, 2779bp, 2925bp, and 2688bp long, respectively, each of them contains a single ORF, the first one (ORF1) encoding a 1115-amino-acid-long protein (122.65 kDa) with a conserved RNA-dependent RNA polymerase (RdRp) motif, its sequences showed the highest identity of only 16.13% to that of the Beauveria bassiana chrysovirus 1. The second ORF (ORF-2) encoding a 807-amino-acid-long coat protein (CP) (88.77 kDa). The virus constitutes a new member of the chrysoviridea family from B. bassiana.

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