In vivo and in vitro decolorization of synthetic dyes by laccase from solid state fermentation with Trametes sp. SYBC-L4

2014 ◽  
Vol 37 (12) ◽  
pp. 2597-2605 ◽  
Author(s):  
Hui-Xing Li ◽  
Rui-Jing Zhang ◽  
Lei Tang ◽  
Jian-Hua Zhang ◽  
Zhong-Gui Mao
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Synthetic Dyes

Degradation of major allergens and allergenicity reduction of soybean meal through solid-state fermentation with microorganisms

2018 ◽  
Vol 9 (3) ◽  
pp. 1899-1909 ◽  
Author(s):  
Anshu Yang ◽  
LingLing Zuo ◽  
Youfei Cheng ◽  
Zhihua Wu ◽  
Xin Li ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Soybean Meal ◽  
Major Allergens

The effect of fermentation on allergenicity of soybean meal was assessed both in vitro and in vivo.

scholarly journals Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Helena Fernandes ◽  
Francisco Moyano ◽  
Carolina Castro ◽  
José Salgado ◽  
Francisca Martínez ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
High Potential ◽  
In Vitro Digestibility ◽  
Brewer’S Spent Grain ◽  
Dietary Phosphorus ◽  
European Seabass ◽  
Spent Grain

AbstractBrewer’s spent grain (BSG) is the largest by-product originated from the brewery industry with a high potential for producing carbohydrases by solid-state fermentation. This work aimed to test the efficacy of a carbohydrases-rich extract produced from solid-state fermentation of BSG, to enhance the digestibility of a plant-based diet for European seabass (Dicentrarchus labrax). First, BSG was fermented with A. ibericus to obtain an aqueous lyophilized extract (SSF-BSG extract) and incorporated in a plant-based diet at increasing levels (0—control; 0.1%, 0.2%, and 0.4%). Another diet incorporating a commercial carbohydrases-complex (0.04%; Natugrain; BASF) was formulated. Then, all diets were tested in in vitro and in vivo digestibility assays. In vitro assays, simulating stomach and intestine digestion in European seabass, assessed dietary phosphorus, phytate phosphorus, carbohydrates, and protein hydrolysis, as well as interactive effects between fish enzymes and dietary SSF-BSG extract. After, an in vivo assay was carried out with European seabass juveniles fed selected diets (0—control; 0.1%, and 0.4%). In vitro digestibility assays showed that pentoses release increased 45% with 0.4% SSF-BSG extract and 25% with Natugrain supplemented diets, while amino acids release was not affected. A negative interaction between endogenous fish enzymes and SSF-BSG extract was observed in both diets. The in vivo digestibility assay corroborated in vitro data. Accordingly, the dietary supplementation with 0.4% SSF-BSG increased the digestibility of dry matter, starch, cellulose, glucans, and energy and did not affect protein digestibility. The present work showed the high potential of BSG to produce an added-value functional supplement with high carbohydrases activity and its potential contribution to the circular economy by improving the nutritional value of low-cost and sustainable ingredients that can be included in aquafeeds.

scholarly journals Feature-Based Molecular Networking Analysis of the Metabolites Produced by in vitro Solid-State Fermentation Reveals Pathways for the Bioconversion of Epigallocatechin Gallate

2020 ◽  
Author(s):  
Hao-Fen Xie ◽  
Ya-Shuai Kong ◽  
Ru-Ze Li ◽  
Hong Zhang ◽  
Lu-Lu Liu ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Epigallocatechin Gallate ◽  
Oxidation Products ◽  
Molecular Networking ◽  
Hygrothermal Effect ◽  
Ring Fission ◽  
Feature Based ◽  
Ring Oxidation

Flavan-3-ol B-ring fission derivatives (FBRFDs) are secondary metabolites that contribute to the unique properties of fermented dark teas. However, the FBRFD precursors and biochemistry are unclear. Fungal strains cultured from Fuzhuan brick tea (FBT) were incubated in an in vitro solid-state fermentation system containing β-cyclodextrin-embedded epigallocatechin gallate (EGCG), a potential precursor of FBRFDs. The produced metabolites were analyzed through a combination of targeted chromatographic isolation, non-targeted spectroscopic identification, and Feature-based Molecular Networking (FBMN) in the Global Natural Products Social Molecular Networking (GNPS) platform. Dihydromyricetin was identified for the first time, indicating that fungi possess a flavan-3-ol C-ring oxidation pathway. EGCG was verified as the precursor of dihydromyricetin and FBRFDs such as teadenol A and fuzhuanin A. The conversion was driven by the fungi rather than a hygrothermal effect. Isolates from Pezizomycotina showed much stronger abilities to convert EGCG to the B-/C-ring oxidation products than those from Saccharomycotina or Basidiomycota.

scholarly journals Solid-state fermentation with orange waste: optimization of Laccase production from Pleurotus pulmonarius CCB-20 and decolorization of synthetic dyes

2020 ◽  
Vol 42 ◽  
pp. e52699 ◽  
Author(s):  
Alex Graça Contato ◽  
Fabíola Dorneles In´ácio ◽  
Tatiane Brugnari ◽  
Caroline Aparecida Vaz de Araújo ◽  
Giselle Maria Maciel ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Fermentation Medium ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Pleurotus Pulmonarius ◽  
Biotechnological Potential ◽  
Orange Waste ◽  
Oxidoreductase Enzymes

Laccases are oxidoreductase enzymes that have the ability to oxidize phenolic substrates. Its biotechnological potential has been greatly explored in many areas as biotechnology industry, bioremediation of dyes, food industry and environmental microbiology. The aim of this study was maximize the laccase production by Pleurotus pulmonarius (Fr.) Quélet in solid-state fermentation (SSF) using orange waste as substrate. After optimization the capability of the crude laccase to decolorize dyes was analyzed. The fermentation medium in the solid-state was optimized by applying a factorial design. After statistics optimization, laccase activity increased two times. The laccase activity appears to be correlated with the ability of crude extract to decolorize some industrial dyes. The optimized laccase was characterized with respect to optimum pH, influence of temperature and salts. Our results demonstrate that P. pulmonarius was an efficient producer of an important industrial enzyme, laccase, in a cheap solid-state system using orange waste as substrate.

Absolute radiotracer concentration measurement using whole-body solid-state SPECT/CT technology: in vivo/in vitro validation

2019 ◽  
Vol 57 (7) ◽  
pp. 1581-1590
Author(s):  
John A. Kennedy ◽  
Ilya Reizberg ◽  
Rachel Lugassi ◽  
Shoham Himmelman ◽  
Zohar Keidar
Keyword(s):  
Solid State ◽  
Concentration Measurement ◽  
Whole Body ◽  
Ct Technology

scholarly journals Decomposition of canola stubble by solid-state fermentation with Cyathus olla

2005 ◽  
Vol 81 (2) ◽  
pp. 87-94 ◽  
Author(s):  
T.C. Shinners-Carnelley ◽  
J.P. Tewari
Keyword(s):  
Cell Wall ◽  
Solid State ◽  
Solid State Fermentation ◽  
Soluble Fraction ◽  
Field Testing ◽  
Cell Wall Composition ◽  
Fiber Content ◽  
Natural Conditions ◽  
Hemicellulose Content

Cyathus olla is being studied for its potential as an inoculant to accelerate decomposition of canola stubble, and hence to reduce the incidence of stubble-borne diseases of this crop. Stubble infested by C. olla appears soft and macerated, but the extent of decomposition incited by this fungus is not known. Composition of stubble fiber of five canola cultivars was determined with the Goering Van Soest method of fiber determination. Sterile canola (cv. Cyclone) substrate was inoculated with C. olla and incubated at 25 C for 45 days, followed by fiber analyses to detect changes in the fiber content. All cultivars were variable with respect to cell wall composition. Canola substrate inoculated with C. olla f. brodiensis had 60.6% of the original lignin remaining after incubation, compared to 65.9% for the substrate incubated with C. olla f. olla and 71.8% with C. olla f. anglicus. Hemicellulose content was reduced as only 75.3, 78.6, and 81.6% of the original hemicellulose content remained after incubation with C. olla f. brodiensis, C. olla f. olla, and C. olla f. anglicus, respectively. Cellulose was also degraded, and the neutral detergent soluble fraction increased. Cyathus olla was capable of degrading lignin in vitro, but field testing must follow to assess its decomposing activity under natural conditions.

scholarly journals Synthesis and Evaluation of Chirally Defined Side Chain Variants of 7-Chloro-4-Aminoquinoline To Overcome Drug Resistance in Malaria Chemotherapy

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Vasantha Rao Dola ◽  
Awakash Soni ◽  
Pooja Agarwal ◽  
Hafsa Ahmad ◽  
Kanumuri Siva Rama Raju ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Solid State ◽  
Side Chain ◽  
Detailed Structure ◽  
Preclinical Development ◽  
Malaria Parasites ◽  
Sar Studies ◽  
Structure Activity

ABSTRACT A novel 4-aminoquinoline derivative [(S)-7-chloro-N-(4-methyl-1-(4-methylpiperazin-1-yl)pentan-2-yl)-quinolin-4-amine triphosphate] exhibiting curative activity against chloroquine-resistant malaria parasites has been identified for preclinical development as a blood schizonticidal agent. The lead molecule selected after detailed structure-activity relationship (SAR) studies has good solid-state properties and promising activity against in vitro and in vivo experimental malaria models. The in vitro absorption, distribution, metabolism, and excretion (ADME) parameters indicate a favorable drug-like profile.

scholarly journals Pengaruh penambahan Aspergillus niger Iradiasi Sinar Gamma Dosis Rendah pada Jerami Padi Fermentasi dan Evaluasi Kualitasnya sebagai Pakan Ternak Ruminansia Secara In Vitro

2017 ◽  
Vol 13 (1) ◽  
pp. 23 ◽  
Author(s):  
Crhisterra E. Kusumaningrum ◽  
Arthalia Poetri Yunisa ◽  
Nana Mulyana ◽  
Suharyono Suharyono
Keyword(s):  
Fatty Acid ◽  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Volatile Fatty Acid ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber

Penelitian dilakukan untuk mengetahui pengaruh pemberian Aspergillus niger iradiasi sinar gamma dosis rendah pada jerami padi fermentasi dan potensinya sebagai pakan ternak ruminansia secara in vitro. Proses fermentasi dilakukan dengan teknik Solid State Fermentation (SSF) menggunakan A. niger yang diiradiasi sinar gamma dosis rendah untuk meningkatkan kemampuan memecah ikatan selulosa. Orientasi dosis iradiasi sinar gamma sebesar 0, 125, 250, 375, 500, 625 dan 750 Gy dilakukan pada A. niger untuk mengetahui kondisi yang optimum dalam menghasilkan aktivitas enzim selulase. A. niger iradiasi sinar gamma pada dosis yang optimum digunakan untuk pembuatan jerami padi fermentasi dan dilakukan evaluasi kemampuan jerami padi fermentasi sebagai pakan ternak ruminansia secara in vitro. Perlakuan jerami padi fermentasi yaitu P0. Jerami Padi, P1. Jerami padi fermentasi A. niger 0 Gy dan P2. Jerami padi fermentasi A. niger 500 Gy. Hasil menunjukkan bahwa dosis optimum iradiasi sinar gamma pada A. niger dalam menghasilkan enzim selulase (3,24 U/ml) adalah 500 Gy. Pada jerami padi fermentasi A. niger 500 Gy menunjukkan aktivitas enzim selulase tertinggi yaitu sebesar 48,5384 U/g bahan kering (BK) dan kadar glukosa sebesar 247,33 mg/g BK pada hari ke-14. Proses fermentasi dengan A. niger iradiasi sinar gamma (500 Gy) mampu meningkatkan kandungan proein kasar, acid detergent fiber (ADF), neutral detergent fiber (NDF) dan bahan kering, namun menurunkan kandungan lemak kasar dan bahan organik (BO). Hasil fermentasi dalam cairan rumen dari jerami padi fermentasi dengan dengan A. niger iradiasi sinar gamma (500 Gy) menunjukkan nilai pH sebesar 7,01; konsentrasi ammonia dan volatile fatty acid (VFA) masing-masing sebesar 3,92% dan 4,73 mmol/100.

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