A novel type of adhering junction in an epithelioid tumorigenic rat cell culture line

1998 ◽  
Vol 294 (1) ◽  
pp. 11-25 ◽  
Author(s):  
M. Schmelz ◽  
Dennis L. Way ◽  
Peter Borgs ◽  
Wiebke K. Peitsch ◽  
Hannelore Schmidt ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Culture Line

Activity of adenos1ne analogs against a cell culture, line resistant to 2-fluoroadenine

1966 ◽  
Vol 15 (11) ◽  
pp. 1719-1728 ◽  
Author(s):  
L.Lee Bennett ◽  
Margaret H. Vail ◽  
Sue Chumley ◽  
John A. Montgomery
Keyword(s):  
Cell Culture ◽  
A Cell ◽  
Cell Culture Line

Elicitor Induction of Cytochrome P-450 Monooxygenases in Cell Suspension Cultures of Chickpea (Cicer arietinum L.) and Their Involvement in Pterocarpan Phytoalexin Biosynthesis

1991 ◽  
Vol 46 (1-2) ◽  
pp. 58-66 ◽  
Author(s):  
Werner Gunia ◽  
Walter Hinderer ◽  
Uta Wittkampf ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Resistant Cultivar ◽  
Susceptible Cultivar ◽  
A Cell ◽  
Cell Culture Line ◽  
Cytochrome P 450

Abstract A yeast glucan elicitor causes the accumulation of the pterocarpan phytoalexins medicarpin and maackiain in chickpea (Cicer arietinum) cell suspension cultures established from seeds. A cell culture line from a chickpea cultivar resistant against its main fungal pathogen Ascochyta rabiei accumulates large amounts (944 nm ol/g fr. wt.) whereas a cell culture line from a susceptible cultivar accumulates only low amounts (38 nm ol/g fr. wt.) of the phytoalexins. This is consistent with differential accumulation of pterocarpan phytoalexins in intact plants [1], The first reactions in the pterocarpan-specific branch of biosynthesis are hydroxylation of the isoflavone intermediate form ononetin in position 2′ or 3′, catalyzed by microsomal cytochrome P-450 monooxygenases. Upon elicitation form ononetin 2′-hydroxylase undergoes a strong transient induction in the cell suspension culture of the resistant cultivar, whereas in the cell culture from the susceptible cultivar it is only slightly induced. In both cell suspension cul­tures the induction of cinnamic acid 4-hydroxylase and of form ononetin 3′-hydroxylase does not show a clear correlation with phytoalexin accumulation. Experiments with different elici­tor concentrations confirm that formononetin 2′-hydroxylase is much more induced in cell cul­tures from the resistant cultivar than from the susceptible one. It is concluded that the massive difference in phytoalexin accumulation between cell suspension cultures from the resistant and susceptible cultivar is determined mainly by the differential induction of form ononetin 2′-hydroxylase activity.

scholarly journals 242 EFFECT OF CAFFEINE ON THE MATURATION OF A TYPE II PNEUMOCYTE-LIKE CELL CULTURE LINE

1981 ◽  
Vol 15 ◽  
pp. 480-480
Author(s):  
Jacob N Finkeistein ◽  
William M Manisealco ◽  
Donald L Shapiro ◽  
Sand H Reuter ◽  
Christina M Kramer
Keyword(s):  
Cell Culture ◽  
Type Ii ◽  
Type Ii Pneumocyte ◽  
Cell Culture Line

A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize

Genome ◽  
2006 ◽  
Vol 49 (11) ◽  
pp. 1399-1407 ◽  
Author(s):  
P. Barret ◽  
M. Brinkman ◽  
M. Beckert
Keyword(s):  
Cell Culture ◽  
Oryza Sativa ◽  
Transcriptional Activation ◽  
Indica Rice ◽  
Doubled Haploid Line ◽  
Plant Genomes ◽  
Somaclonal Variations ◽  
Form Part ◽  
Cell Culture Line

Miniature inverted-repeat transposable elements (MITEs) are nonautonomous elements that are abundant in plant genomes. The rice MITE mPing was shown to be mobilized by anther culture, and the associated transposon Pong was shown to transpose actively in an Oryza sativa ‘indica’ rice cell-culture line. We have identified 3 sequences in maize named ZmTPAPong-like 1, 2, and 3 that displayed homology with the transposase of Pong. Here, we show that these sequences are differentially expressed during the in vitro androgenetic process in maize. We also demonstrate that the ZmTPAPong-like 1 and 3 sequences reveal somaclonal variations among plants regenerated from the calli of a doubled haploid line. These data suggest that the ZmTPAPong-like sequences could form part of a Zea mays element related to the rice Pong element. The possible activation of this newly discovered element under stress conditions is discussed.

scholarly journals Cultivation ofEhrlichia Canisin a Continuous BALB/C Mouse Macrophage Cell Culture Line

2001 ◽  
Vol 13 (6) ◽  
pp. 521-523 ◽  
Author(s):  
Avi Keysary ◽  
Trevor Waner ◽  
Carmella Strenger ◽  
Shimon Harrus
Keyword(s):  
Cell Culture ◽  
Mouse Macrophage ◽  
Macrophage Cell ◽  
Cell Culture Line
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