A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize

Genome ◽  
2006 ◽  
Vol 49 (11) ◽  
pp. 1399-1407 ◽  
Author(s):  
P. Barret ◽  
M. Brinkman ◽  
M. Beckert
Keyword(s):  
Cell Culture ◽  
Oryza Sativa ◽  
Transcriptional Activation ◽  
Indica Rice ◽  
Doubled Haploid Line ◽  
Plant Genomes ◽  
Somaclonal Variations ◽  
Form Part ◽  
Cell Culture Line

Miniature inverted-repeat transposable elements (MITEs) are nonautonomous elements that are abundant in plant genomes. The rice MITE mPing was shown to be mobilized by anther culture, and the associated transposon Pong was shown to transpose actively in an Oryza sativa ‘indica’ rice cell-culture line. We have identified 3 sequences in maize named ZmTPAPong-like 1, 2, and 3 that displayed homology with the transposase of Pong. Here, we show that these sequences are differentially expressed during the in vitro androgenetic process in maize. We also demonstrate that the ZmTPAPong-like 1 and 3 sequences reveal somaclonal variations among plants regenerated from the calli of a doubled haploid line. These data suggest that the ZmTPAPong-like sequences could form part of a Zea mays element related to the rice Pong element. The possible activation of this newly discovered element under stress conditions is discussed.

scholarly journals Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein

2004 ◽  
Vol 78 (5) ◽  
pp. 2637-2641 ◽  
Author(s):  
Su-Jun Deng ◽  
Kenneth H. Pearce ◽  
Eric P. Dixon ◽  
Kelly A. Hartley ◽  
Thomas B. Stanley ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Papillomavirus ◽  
Dna Replication ◽  
Transcriptional Activation ◽  
Synthetic Peptides ◽  
Dna Interaction ◽  
Peptide Library ◽  
Random Peptide Library ◽  
Random Peptide

ABSTRACT Peptide antagonists of the human papillomavirus type 11 (HPV-11) E2-DNA association were identified using a filamentous bacteriophage random peptide library. Synthetic peptides antagonized the E2-DNA interaction, effectively blocked E2-mediated transcriptional activation of a reporter gene in cell culture, and inhibited E1-E2-mediated HPV-11 DNA replication in vitro. These peptides may prove to be useful tools for characterizing E2 function and for exploring the effectiveness of E2-inhibitor-based treatments for HPV-associated diseases.

In vitro flowering of indica rice (Oryza sativa L. spp. indica)

2012 ◽  
Vol 48 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Suriyan Cha-um ◽  
Thapanee Samphumphuang ◽  
Chalermpol Kirdmanee
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Flowering

scholarly journals Formulation of Ocular In Situ Gels with Lithuanian Royal Jelly and Their Biopharmaceutical Evaluation In Vitro

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3552
Author(s):  
Kristina Perminaite ◽  
Mindaugas Marksa ◽  
Monika Stančiauskaitė ◽  
Tadas Juknius ◽  
Aidas Grigonis ◽  
...  
Keyword(s):  
Cell Culture ◽  
Refractive Index ◽  
Cell Viability ◽  
Royal Jelly ◽  
Biological Activities ◽  
Pharmaceutical Formulations ◽  
Exposure Test ◽  
Cell Culture Line

Royal jelly is a natural substance produced by worker bees that possesses a variety of biological activities, including antioxidant, anti-inflammatory, antibacterial, and protective. Although fresh royal jelly is kept at low temperatures, to increase its stability, it needs to be incorporated into pharmaceutical formulations, such as in situ gels. The aim of this study was to formulate in situ ocular gels containing Lithuanian royal jelly for topical corneal use in order to increase the retention time of the formulation on the ocular surface and bioavailability. Gels were evaluated for physicochemical characteristics (pH, rheological properties, refractive index) and in vitro drug release measuring the amount of 10-hydroxy-2-decenoic acid (10-HDA). An ocular irritation test and cell viability tests were performed using the SIRC (Statens Seruminstitut Rabbit Cornea) cell culture line. Results indicated that all the in situ gels were within an acceptable pH and refractive index range close to corneal properties. Rheology studies have shown that the gelation temperature varies between 25 and 32 °C, depending on the amount of poloxamers. The release studies have shown that the release of 10-HDA from in situ gels is more sustained than royal jelly suspension. All gel formulations were non-irritant according to the short-time exposure test (STE) using the SIRC cell culture line, and long-term cell viability studies indicated that the formulations used in small concentrations did not induce cell death. Prepared in situ gels containing royal jelly have potential for ocular drug delivery, and they may improve the bioavailability, stability of royal jelly, and formation of non-irritant ocular formulations.

Effect of Exogenous Glucose and Abscisic Acid on Physiological and Morphological Performances of in vitro Indica Rice (Oryza sativa L. sp. indica)

2007 ◽  
Vol 2 (2) ◽  
pp. 155-166 ◽  
Author(s):  
Suriyan Cha-um ◽  
Sittiruk Roytakul ◽  
Thamakron Sathung ◽  
Atikhun Maijandang ◽  
Chalermpol Kirdmanee
Keyword(s):  
Oryza Sativa ◽  
Abscisic Acid ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Exogenous Glucose
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