Methodological aspects of assessing phagocytosis of Vibrio anguillarum by leucocytes of gilthead seabream ( Sparus aurata L.) by flow cytometry and electron microscopy

1998 ◽  
Vol 293 (1) ◽  
pp. 133-141 ◽  
Author(s):  
M. A. Esteban ◽  
V. Mulero ◽  
J. Muñoz ◽  
J. Meseguer
Keyword(s):  
Electron Microscopy ◽  
Flow Cytometry ◽  
Sparus Aurata ◽  
Vibrio Anguillarum ◽  
Gilthead Seabream ◽  
Methodological Aspects

scholarly journals Occurrence of Neobenedenia girellae (Monogenea: Capsalidae) in Gilthead Seabream Sparus aurata (Actinopterygii: Sparidae) Cultured in Portugal

Pathogens ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1269
Author(s):  
Perla Tedesco ◽  
Monica Caffara ◽  
Nuno Miguel Ribeiro Moreira ◽  
César Gomes ◽  
Andrea Gustinelli ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Molecular Analysis ◽  
Morphological Analysis ◽  
Sparus Aurata ◽  
Species Level ◽  
Gilthead Seabream ◽  
Potential Threat ◽  
Definition Of ◽  
Scanning Electron ◽  
Specific Identity

Monogenean capsalids of the genus Neobenedenia are widespread parasites of wild and farmed marine fish, and represent a potential threat to mariculture due to their pathogenicity and ability to cause mortality in fish maintained in controlled conditions. The identification of Neobenedenia species and, consequently, the definition of their host specificity is often problematic due to their highly conserved morphology; therefore, in order to establish their specific identity, microscopic observation should be complemented with molecular analysis. The present work aims at characterizing Neobenedenia specimens infecting the skin of cage reared gilthead seabream Sparus aurata from Portugal. Parasite samples obtained from caged fish were processed for morphological analysis, through observation in light and scanning electron microscopy, and for molecular analysis, through amplification and sequencing of 28S rDNA and cytB, aimed at identifying them to the species level. Our results showed that the collected parasites belonged to the species Neobenedenia girellae; the susceptibility of S. aurata towards this pathogenic capsalid monogenean highlighted in the present work represents an important risk in the farming of this valuable fish species.

Saccharomyces cerevisiae var. boulardii preserves the integrity of intestinal mucosa in gilthead seabream, Sparus aurata subjected to a bacterial challenge with Vibrio anguillarum

2015 ◽  
Vol 48 (2) ◽  
pp. 725-728 ◽  
Author(s):  
Giulia Maricchiolo ◽  
Letteria Caccamo ◽  
Monique Mancuso ◽  
Giovanni Marco Cusimano ◽  
Francesco Gai ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Intestinal Mucosa ◽  
Sparus Aurata ◽  
Vibrio Anguillarum ◽  
Gilthead Seabream ◽  
Bacterial Challenge

scholarly journals Flow cytometry based techniques to study testicular acidophilic granulocytes from the protandrous fish gilthead seabream (Sparus aurata L.)

10.1251/bpo81 ◽  
2004 ◽  
Vol 6 (1) ◽  
pp. 129-136 ◽  
Author(s):  
Elena Chaves-Pozo ◽  
Victoriano Mulero ◽  
José Meseguer ◽  
Alfonsa García Ayala
Keyword(s):  
Flow Cytometry ◽  
Sparus Aurata ◽  
Gilthead Seabream

Acidophilic granulocytes of the marine fish gilthead seabream ( Sparus aurata L.) produce interleukin-1� following infection with Vibrio anguillarum

2004 ◽  
Vol 316 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Elena Chaves-Pozo ◽  
Pablo Pelegr�n ◽  
Jes�s Garc�a-Castillo ◽  
Alfonsa Garc�a-Ayala ◽  
Victoriano Mulero ◽  
...  
Keyword(s):  
Marine Fish ◽  
Sparus Aurata ◽  
Interleukin 1 ◽  
Vibrio Anguillarum ◽  
Gilthead Seabream
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