Organization of the intercellular spaces of porcine epidermal and palatal stratum corneum: a quantitative study employing ruthenium tetroxide

1995 ◽  
Vol 279 (2) ◽  
pp. 271-276 ◽  
Author(s):  
D. C. Swartzendruber ◽  
A. Manganaro ◽  
K. C. Madison ◽  
M. Kremer ◽  
P. W. Wertz ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Quantitative Study ◽  
Intercellular Spaces ◽  
Ruthenium Tetroxide

Organization of the intercellular spaces of porcine epidermal and palatal stratum corneum: a quantitative study employing ruthenium tetroxide

1995 ◽  
Vol 279 (2) ◽  
pp. 271-276 ◽  
Author(s):  
D. C. Swartzendruber ◽  
A. Manganaro ◽  
K. C. Madison ◽  
M. Kremer ◽  
P. W. Wertz ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Quantitative Study ◽  
Intercellular Spaces ◽  
Ruthenium Tetroxide

scholarly journals The histochemical distribution of protein bound sulfhydryl groups in human epidermis by the new staining method.

1979 ◽  
Vol 27 (5) ◽  
pp. 942-946 ◽  
Author(s):  
H Ogawa ◽  
A Taneda ◽  
Y Kanaoka ◽  
T Sekine
Keyword(s):  
Cell Membrane ◽  
Stratum Corneum ◽  
Staining Method ◽  
Sulfhydryl Groups ◽  
The Other ◽  
Human Epidermis ◽  
Horny Layer ◽  
Intercellular Spaces ◽  
Sh Groups ◽  
Thiol Reagent

Recently, we synthesized a new fluorescent thiol reagent, N-(7-dimethylamino-4-methylcoumarinyl)-maleimide (DACM) which is nonfluorescent by itself but will react readily with -SH groups to form highly fluorescent addition products. By the use of this reagent, we studied the localization and concentration of -SH groups and S--S linkages in the human epidermis. The distribution of -SH groups in living layers was abundant in cytoplasm but not in nuclei. The fluorescence was concentrated on the cell membrane or intercellular spaces (MIC parts) and was increased at the spino-granular junction. In the horny layer, the fluorescence of the MIC parts appeared brilliantly in the lower layers and decreased gradually. On the other hand, the fluorescence of cytoplasm in keratinized cells in the stratum corneum was faint. The localization of S--S linkages was not a characteristic of the living layers, but appeared abruptly at the junction of living and horny layers. The fluorescence was localized to the MIC parts and disappeared gradually. The distribution of S--S linkages appeared to be very low in the cytoplasm of keratinized cells. No substantial fluorescence was localized on keratohyalin granules even after reduction.

Quantitative study of stratum corneum ceramides contents in patients with sensitive skin

2011 ◽  
Vol 39 (3) ◽  
pp. 295-300 ◽  
Author(s):  
Hee Jin CHO ◽  
Bo Young CHUNG ◽  
Hee Bong LEE ◽  
Hye One KIM ◽  
Chun Wook PARK ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Quantitative Study ◽  
Sensitive Skin

scholarly journals CELL JUNCTIONS IN AMPHIBIAN SKIN

1965 ◽  
Vol 26 (1) ◽  
pp. 263-291 ◽  
Author(s):  
Marilyn G. Farquhar ◽  
George E. Palade
Keyword(s):  
Stratum Corneum ◽  
Membrane Fusion ◽  
Water Soluble ◽  
Cell Junctions ◽  
Physiological Data ◽  
Junctional Complex ◽  
Stratum Granulosum ◽  
Intercellular Spaces ◽  
Outermost Layer ◽  
Zonulae Occludentes

Cell junctions have been investigated in the amphibian epidermis, a stratified squamous epithelium, and compared to those described previously in simple columnar epithelia of mammalian cavitary organs. In adult frogs and toads, and in larvae approaching metamorphosis, belts of membrane fusion or zonulae occludentes of considerable depth are regularly found between adjoining cells of the outermost layer of the stratum corneum, binding the cells together into a continuous, uninterrupted sheet. Another set of occluding zonules appears in the second cornified layer (when such a layer is present), and a third set usually occurs in the outermost layer of the stratum granulosum. Specialized elements described as "modified" and "composite" desmosomes are encountered along the lateral and basal aspects, respectively, of the cornified cells; ordinary desmosomes and maculae occludentes (i.e., spots of membrane fusion) are found in all other strata. The usual 200 A intercellular gap is generally maintained between the cells of the stratum germinativum at the basal ends of the intercellular spaces. Hence, the intercellular spaces of the epidermis form a largely continuous network, closed to the external medium and open to the dermal interstitia. The situation is comparable to that found in columnar epithelia, except that the intercellular spaces are much more extensive, and an extracellular subcompartment (or two) apparently exists in the stratum corneum and between the latter and the stratum granulosum. The last subcompartment is usually filled with a dense substance, probably derived from discharged secretory granules. The tripartite junctional complex characteristic of lumen-lining epithelia (i.e., a zonula occludens followed by a zonula adhaerens, and desmosome) is seen only in early larvae; in adults and in larvae approaching metamorphosis, the occluding zonule is followed directly by a series of modified desmosomes. Interpreted in the light of current physiological data, these findings suggest that the diffusion of water, ions, and small, water-soluble molecules is impeded along the intercellular spaces of the epidermis by zonulae occludentes while it is facilitated from cell to cell within the epidermis by zonulae and maculae occludentes.

Basal Lamina Formation in DMBA Induced Mammary Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 534-535
Author(s):  
I. Russo ◽  
J. Saby ◽  
J. Russo
Keyword(s):  
Mammary Carcinoma ◽  
Virgin Female ◽  
Mammary Glands ◽  
Sesame Oil ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Intermediate Type ◽  
Sprague Dawley ◽  
Intercellular Spaces

It has been previously demonstrated that DMBA-induced rat mammary carcinoma originates in the terminal end bud (TEB) of the mammary gland by proliferation of intermediate type cells (1). The earliest lesion identified is the intraductal proliferation (IDP), which gives rise to intraductal carcinomas. These evolve to cribriform, papillary and comedo types (2). In the present work, we report the ultrastructural changes that take place in the IDP for the formation of a cribriform pattern.Fifty-five-day-old Sprague Dawley virgin female rats were inoculated intra- gastrically with 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) in 1 ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from both control and experimental rats were removed weekly from the time of inoculation until 86 days post-inoculation. The glands were fixed and processed for electron microscopy (2).The first change observed in IDP's was the widening of intercellular spaces and the secretion of an electron dense material into these spaces (Fig. 1).

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