The involvement of the RAD6 gene in starvation-induced reverse mutation in Saccharomyces cerevisiae

1998 ◽  
Vol 258 (5) ◽  
pp. 546-552 ◽  
Author(s):  
Z. Storchová ◽  
A. P. Rojas Gil ◽  
B. Janderová ◽  
V. Vondrejs
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reverse Mutation ◽  
Rad6 Gene

RAD6+ gene of Saccharomyces cerevisiae codes for two mutationally separable deoxyribonucleic acid repair functions

1981 ◽  
Vol 1 (2) ◽  
pp. 153-157
Author(s):  
M F Tuite ◽  
B S Cox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Lethal Effects ◽  
Rad6 Gene ◽  
Ultraviolet Sensitivity ◽  
Induced Mutagenesis ◽  
Error Prone Repair

The response of two mutant alleles of the RAD6+ gene of Saccharomyces cerevisiae to the ochre translational suppressor SUQ5 was determined. Both the ultraviolet sensitivity phenotype and the deficiency in ultraviolet-induced mutagenesis phenotype of the rad6-1 allele were suppressed in a [psi+] background. For the rad6-3 allele, only the ultraviolet-sensitivity phenotype was suppressible in a [psi+] background. An SUQ5 rad6-3 [psi+] strain that was examined showed the normal rad6-3 deficiency in ultraviolet-induced mutagenesis. We propose that the RAD6+ gene is divided into two cistrons, RAD6A and RAD6B. RAD6A codes for an activity responsible for the error-prone repair of ultraviolet-induced lesions in deoxyribonucleic acid but is not involved in a cell's resistance to the lethal effects of ultraviolet light. RAD6B codes for an activity essential for error-free repair of potentially lethal mutagenic damage.

scholarly journals RAD6+ gene of Saccharomyces cerevisiae codes for two mutationally separable deoxyribonucleic acid repair functions.

1981 ◽  
Vol 1 (2) ◽  
pp. 153-157 ◽  
Author(s):  
M F Tuite ◽  
B S Cox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Lethal Effects ◽  
Rad6 Gene ◽  
Ultraviolet Sensitivity ◽  
Induced Mutagenesis ◽  
Error Prone Repair

The response of two mutant alleles of the RAD6+ gene of Saccharomyces cerevisiae to the ochre translational suppressor SUQ5 was determined. Both the ultraviolet sensitivity phenotype and the deficiency in ultraviolet-induced mutagenesis phenotype of the rad6-1 allele were suppressed in a [psi+] background. For the rad6-3 allele, only the ultraviolet-sensitivity phenotype was suppressible in a [psi+] background. An SUQ5 rad6-3 [psi+] strain that was examined showed the normal rad6-3 deficiency in ultraviolet-induced mutagenesis. We propose that the RAD6+ gene is divided into two cistrons, RAD6A and RAD6B. RAD6A codes for an activity responsible for the error-prone repair of ultraviolet-induced lesions in deoxyribonucleic acid but is not involved in a cell's resistance to the lethal effects of ultraviolet light. RAD6B codes for an activity essential for error-free repair of potentially lethal mutagenic damage.

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