Recombination and mutagenesis in rad6 mutants of Saccharomyces cerevisiae: Evidence for multiple functions of the RAD6 gene

1981 ◽  
Vol 184 (3) ◽  
pp. 410-415 ◽  
Author(s):  
Beth A. Montelone ◽  
Satya Prakash ◽  
Louise Prakash
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Multiple Functions ◽  
Rad6 Gene

scholarly journals Protein Glycosylation inAspergillus fumigatusIs Essential for Cell Wall Synthesis and Serves as a Promising Model of Multicellular Eukaryotic Development

2012 ◽  
Vol 2012 ◽  
pp. 1-21 ◽  
Author(s):  
Cheng Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Posttranslational Modification ◽  
Mammalian Cells ◽  
Protein Glycosylation ◽  
Cell Wall Synthesis ◽  
Fungal Cell ◽  
Multiple Functions ◽  
Significant Attention

Glycosylation is a conserved posttranslational modification that is found in all eukaryotes, which helps generate proteins with multiple functions. Our knowledge of glycosylation mainly comes from the investigation of the yeastSaccharomyces cerevisiaeand mammalian cells. However, during the last decade, glycosylation in the human pathogenic moldAspergillus fumigatushas drawn significant attention. It has been revealed that glycosylation inA. fumigatusis crucial for its growth, cell wall synthesis, and development and that the process is more complicated than that found in the budding yeastS. cerevisiae. The present paper implies that the investigation of glycosylation inA. fumigatusis not only vital for elucidating the mechanism of fungal cell wall synthesis, which will benefit the design of new antifungal therapies, but also helps to understand the role of protein glycosylation in the development of multicellular eukaryotes. This paper describes the advances in functional analysis of protein glycosylation inA. fumigatus.

Multiple functions for sterols in Saccharomyces cerevisiae

1985 ◽  
Vol 837 (3) ◽  
pp. 336-343 ◽  
Author(s):  
Russell J. Rodriguez ◽  
Christopher Low ◽  
Cynthia D.K. Bottema ◽  
Leo W. Parks
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Multiple Functions

Multiple functions of the vacuolar sorting protein Ccz1p in Saccharomyces cerevisiae

2005 ◽  
Vol 329 (1) ◽  
pp. 197-204 ◽  
Author(s):  
Marta Hoffman-Sommer ◽  
Andrzej Migdalski ◽  
Joanna Rytka ◽  
Róża Kucharczyk
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Multiple Functions ◽  
Vacuolar Sorting

RAD6+ gene of Saccharomyces cerevisiae codes for two mutationally separable deoxyribonucleic acid repair functions

1981 ◽  
Vol 1 (2) ◽  
pp. 153-157
Author(s):  
M F Tuite ◽  
B S Cox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Lethal Effects ◽  
Rad6 Gene ◽  
Ultraviolet Sensitivity ◽  
Induced Mutagenesis ◽  
Error Prone Repair

The response of two mutant alleles of the RAD6+ gene of Saccharomyces cerevisiae to the ochre translational suppressor SUQ5 was determined. Both the ultraviolet sensitivity phenotype and the deficiency in ultraviolet-induced mutagenesis phenotype of the rad6-1 allele were suppressed in a [psi+] background. For the rad6-3 allele, only the ultraviolet-sensitivity phenotype was suppressible in a [psi+] background. An SUQ5 rad6-3 [psi+] strain that was examined showed the normal rad6-3 deficiency in ultraviolet-induced mutagenesis. We propose that the RAD6+ gene is divided into two cistrons, RAD6A and RAD6B. RAD6A codes for an activity responsible for the error-prone repair of ultraviolet-induced lesions in deoxyribonucleic acid but is not involved in a cell's resistance to the lethal effects of ultraviolet light. RAD6B codes for an activity essential for error-free repair of potentially lethal mutagenic damage.

scholarly journals RAD6+ gene of Saccharomyces cerevisiae codes for two mutationally separable deoxyribonucleic acid repair functions.

1981 ◽  
Vol 1 (2) ◽  
pp. 153-157 ◽  
Author(s):  
M F Tuite ◽  
B S Cox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ultraviolet Light ◽  
Deoxyribonucleic Acid ◽  
Lethal Effects ◽  
Rad6 Gene ◽  
Ultraviolet Sensitivity ◽  
Induced Mutagenesis ◽  
Error Prone Repair

The response of two mutant alleles of the RAD6+ gene of Saccharomyces cerevisiae to the ochre translational suppressor SUQ5 was determined. Both the ultraviolet sensitivity phenotype and the deficiency in ultraviolet-induced mutagenesis phenotype of the rad6-1 allele were suppressed in a [psi+] background. For the rad6-3 allele, only the ultraviolet-sensitivity phenotype was suppressible in a [psi+] background. An SUQ5 rad6-3 [psi+] strain that was examined showed the normal rad6-3 deficiency in ultraviolet-induced mutagenesis. We propose that the RAD6+ gene is divided into two cistrons, RAD6A and RAD6B. RAD6A codes for an activity responsible for the error-prone repair of ultraviolet-induced lesions in deoxyribonucleic acid but is not involved in a cell's resistance to the lethal effects of ultraviolet light. RAD6B codes for an activity essential for error-free repair of potentially lethal mutagenic damage.

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