Sesqui-Ds, the chromosome-breaking insertion at bz-m1, links double Ds to the original Ds element

1997 ◽  
Vol 255 (6) ◽  
pp. 580-586 ◽  
Author(s):  
I. M. Martínez-Férez ◽  
H. K. Dooner
Keyword(s):  
Ds Element ◽  
Chromosome Breaking

scholarly journals Mapping of transgenic alleles in soybean using a nanopore-based sequencing strategy

2019 ◽  
Vol 70 (15) ◽  
pp. 3825-3833 ◽  
Author(s):  
Shengjun Li ◽  
Shangang Jia ◽  
Lili Hou ◽  
Hanh Nguyen ◽  
Shirley Sato ◽  
...  
Keyword(s):  
High Throughput ◽  
Soybean Genome ◽  
Transgenic Technology ◽  
Transgenic Soybean ◽  
Pooled Dna ◽  
Sequencing Strategy ◽  
Ds Element ◽  
Soybean Plants ◽  
Cost Efficient ◽  
Transgene Insertion

Abstract Transgenic technology was developed to introduce transgenes into various organisms to validate gene function and add genetic variations >40 years ago. However, the identification of the transgene insertion position is still challenging in organisms with complex genomes. Here, we report a nanopore-based method to map the insertion position of a Ds transposable element originating in maize in the soybean genome. In this method, an oligo probe is used to capture the DNA fragments containing the Ds element from pooled DNA samples of transgenic soybean plants. The Ds element-enriched DNAs are then sequenced using the MinION-based platform of Nanopore. This method allowed us to rapidly map the Ds insertion positions in 51 transgenic soybean lines through a single sequencing run. This strategy is high throughput, convenient, reliable, and cost-efficient. The transgenic allele mapping protocol can be easily translated to other eukaryotes with complex genomes.

scholarly journals Gametophyte-specific transposition of the maize Ds element in transgenic tobacco

1996 ◽  
Vol 10 (3) ◽  
pp. 569-578 ◽  
Author(s):  
Simon Firek ◽  
David J. Martin ◽  
Michael R. Roberts ◽  
Faye Sturgess ◽  
Rod Scott ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Ds Element

scholarly journals Regulation of Epstein-Barr Virus OriP Replication by Poly(ADP-Ribose) Polymerase 1

2010 ◽  
Vol 84 (10) ◽  
pp. 4988-4997 ◽  
Author(s):  
Italo Tempera ◽  
Zhong Deng ◽  
Constandache Atanasiu ◽  
Chi-Ju Chen ◽  
Maria D'Erme ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Epstein Barr Virus ◽  
Origin Recognition Complex ◽  
Structural Changes ◽  
Replication Initiation ◽  
Barr Virus ◽  
Ds Element ◽  
Epstein Barr

ABSTRACT Poly(ADP-ribose) polymerase (PARP) is an abundant, chromatin-associated, NAD-dependent enzyme that functions in multiple chromosomal processes, including DNA replication and chromatin remodeling. The Epstein-Barr virus (EBV) origin of plasmid replication (OriP) is a dynamic genetic element that confers stable episome maintenance, DNA replication initiation, and chromatin organization functions. OriP function depends on the EBV-encoded origin binding protein EBNA1. We have previously shown that EBNA1 is subject to negative regulation by poly(ADP-ribosyl)ation (PARylation). We now show that PARP1 physically associates with OriP in latently EBV-infected B cells. Short hairpin RNA depletion of PARP1 enhances OriP replication activity and increases EBNA1, origin recognition complex 2 (ORC2), and minichromosome maintenance complex (MCM) association with OriP. Pharmacological inhibitors of PARP1 enhance OriP plasmid maintenance and increase EBNA1, ORC2, and MCM3 occupancy at OriP. PARylation in vitro inhibits ORC2 recruitment and remodels telomere repeat factor (TRF) binding at the dyad symmetry (DS) element of OriP. Purified PARP1 can ribosylate EBNA1 at multiple sites throughout its amino terminus but not in the carboxy-terminal DNA binding domain. We also show that EBNA1 linking regions (LR1 and LR2) can bind directly to oligomers of PAR. We propose that PARP1-dependent PARylation of EBNA1 and adjacently bound TRF2 induces structural changes at the DS element that reduce EBNA1 DNA binding affinity and functional recruitment of ORC.

scholarly journals Ds-INDUCED ALLELES AT THE OPAQUE-2 LOCUS OF MAIZE

Genetics ◽  
1986 ◽  
Vol 112 (1) ◽  
pp. 121-133
Author(s):  
Mario Motto ◽  
Renzo Marotta ◽  
Natale Di Fonzo ◽  
Carlo Soave ◽  
Francesco Salamini
Keyword(s):  
Transposable Elements ◽  
Transposon Mutagenesis ◽  
Dna Fragments ◽  
Wild Type ◽  
Genetic Analyses ◽  
Somatic Instability ◽  
Mutable Allele ◽  
Recessive Phenotype ◽  
Maize Plants ◽  
Ds Element

ABSTRACT Transposon mutagenesis has been used to isolate mutable alleles at the Opaque-2 (O2) locus of maize. Plants with the Activator-Dissociation (Ac-Ds) system of transposable elements and O2 were crossed as males to a stable o2 tester line. Among a population of 200,000 kernels, 198 exceptional kernels with somatic instability were recovered. In four cases, designated O2-m1, o2-m2, O2-m3 and O2-m4, variegated phenotypes appeared in F2 and subsequent generations. Genetic analyses indicated that the presence of Ds near or within the O2 gene was responsible for the observed somatic instability at the O2 locus. The phenotypes of the newly induced alleles were of two types. Alleles O2-m1, O2-m3 and O2-m4, in the absence of Ac, were characterized by kernel phenotypes indistinguishable from the wild type; in the presence of Ac they generated kernels with opaque sectors interspersed within a vitreous background. In contrast, the mutable allele o2-m2, in the absence of Ac, was characterized by kernels with a recessive phenotype similar to o2 recessive mutants. In the presence of Ac, it reverted somatically to wild-type-producing kernels with vitreous spots in an o2 background. The association of the Ds element with the O2 locus may prove a valuable tool directed to the isolation of DNA fragments bearing the O2 gene.

Regional mapping of the gene coding for enolase-2 on human chromosome 12

1982 ◽  
Vol 53 (1) ◽  
pp. 245-254
Author(s):  
M.L. Law ◽  
F.T. Kao
Keyword(s):  
Human Chromosome ◽  
Linear Order ◽  
Segregation Pattern ◽  
Chromosome 12 ◽  
Regional Mapping ◽  
Neuronal Tissue ◽  
Gene Coding ◽  
Mapping Analysis ◽  
Chromosome Breaking ◽  
Hybrid Clones

Enolase-2 (ENO2), previously termed 14-3-2 protein, is an isozyme of enolase that is enriched in neuronal tissue. The gene coding for ENO2 was previously assigned to human chromosome 12. The present study presents data for a regional mapping of gene ENO2 using cell hybrids containing various deletions of human chromosome 12. These deletions were produced by treatment with chromosome-breaking agents. Cytogenetic analysis has allowed assignment of ENO2 to the short arm of chromosome 12, in the region of pter-p1205. This assignment is consistent with the segregation pattern of the 93 hybrid clones analysed. The segregation pattern has also established the linear order of 6 genes on chromosome 12:pter-TPI-GAPD-LDHB-ENO2-centromere-SHMT-PEPB-qter. Estimation of the relative distances between the 6 genes on chromosome 12 has been made by a statistical mapping analysis of the segregation data of the hybrid clones. A set of deletion hybrids containing various combinations of these 6 markers has been established for a rapid regional mapping of genes in one of these regions on chromosome 12.

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