Isolation and identification by partial sequencing of the 18S ribosomal gene of free-living amoebae from necrotic tissue of Basiliscus plumifrons (Sauria: Iguanidae)

1999 ◽  
Vol 85 (7) ◽  
pp. 601-603 ◽  
Author(s):  
Julia Walochnik ◽  
Andreas Hassl ◽  
Kirsten Simon ◽  
Gerald Benyr ◽  
Horst Aspöck
Keyword(s):  
Ribosomal Gene ◽  
Necrotic Tissue ◽  
Isolation And Identification ◽  
Free Living ◽  
Partial Sequencing

scholarly journals Isolation and identification of free living amoeba (Naegleria and Acanthamoeba) in Shiraz water resources by morphological criteria

2012 ◽  
Vol 10 (3) ◽  
pp. 33-42 ◽  
Author(s):  
Shekofeh Ghadar-ghadr ◽  
Kavous Solhjoo ◽  
Mohammadjavad Norouz-nejad ◽  
Reyhaneh Rohi ◽  
Samaneh Zia-Jahromi ◽  
...  
Keyword(s):  
Water Resources ◽  
Isolation And Identification ◽  
Free Living ◽  
Morphological Criteria ◽  
Free Living Amoeba

scholarly journals Isolation and identification of free-living amoeba from the hot springs and beaches of the Caspian Sea

2020 ◽  
Vol 10 ◽  
pp. e00151
Author(s):  
Alireza Latifi ◽  
Mahboobeh Salami ◽  
Elham Kazemirad ◽  
Mohammad Soleimani
Keyword(s):  
Caspian Sea ◽  
Hot Springs ◽  
The Caspian Sea ◽  
Isolation And Identification ◽  
Free Living ◽  
Free Living Amoeba

scholarly journals IDENTIFICATION OF ENDOPHYTIC BACTERIA OF SEEDS FROM Cedrela odorata L. (Meliaceae) WITH BIOTECHNOLOGICAL CHARACTERISTICS

2021 ◽  
Vol 26 (2) ◽  
pp. 196-206
Author(s):  
Saúl Espinosa Zaragoza ◽  
Ricardo Sánchez Cruz ◽  
Diana Sanzón Gómez ◽  
Margarita C Escobar Sandoval ◽  
Gustavo Yañez Ocampo ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Carbon Sources ◽  
Phytopathogenic Fungi ◽  
Ribosomal Gene ◽  
Cedrela Odorata ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Xenobiotic Compounds ◽  
Phosphate Solubilizing

In the present study, 62 endophytic bacterial strains of cedar seeds (Cedrela odorataL.), collected in the municipalities of Huehuetán, Motozintla, and Pijijiapan in the state of Chiapas, Mexico were isolated. The goal was to identify characteristics of biotechnological interest such as biocontrol, promotion of plant growth, and growth in aromatic compounds. The strains were identified by the partial sequence of the 16S ribosomal gene as belonging to the Bacillusgenus. The biocontrol capacity of phytopathogenic fungi, production of indoleacetic acid (IAA), solubilization of phosphate, and growth in xenobiotic compounds (phenanthrene, benzene, anthracene, or phenol) were detected in 26 strains of the 62 isolates. 21 % of the strains inhibited the mycelial growth of Alternaria solaniand Fusariumsp., and 13 % of the Phytophthora capsicioomycete. IAA production was detected in 24 isolates, phosphate solubilizing activity was identified in 18 isolates, while the ability to grow in the presence of phenanthrene and benzene was found in 26 isolates; 24 isolates grew in the presence of anthracene and only two isolates grew in phenol as the only carbon sources. This is the first report of the isolation and identification of endophytic bacteria from cedar seeds, where biotechnological characteristics were detected for biological control, promotion of plant growth, and growth in the presence of xenobiotic compounds.

scholarly journals Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Kübra Açıkalın Coşkun ◽  
Semra Özçelik ◽  
Lütfi Tutar ◽  
Nazif Elaldı ◽  
Yusuf Tutar
Keyword(s):  
18S Rdna ◽  
Morphological Analysis ◽  
Tap Water ◽  
Acanthamoeba Castellanii ◽  
Isolation And Identification ◽  
Free Living ◽  
Rdna Gene ◽  
Hartmannella Vermiformis ◽  
Sequence Types ◽  
18S Rdna Gene

The present work focuses on a local survey of free-living amoebae (FLA) that cause opportunistic and nonopportunistic infections in humans. Determining the prevalence of FLA in water sources can shine a light on the need to prevent FLA related illnesses. A total of 150 samples of tap water were collected from six districts of Sivas province. The samples were filtered and seeded on nonnutrient agar containingEscherichia colispread. Thirty-three (22%) out of 150 samples were found to be positive for FLA. The FLA were identified by morphology and by PCR using 18S rDNA gene. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of three different species,Acanthamoeba castellanii,Acanthamoeba polyphaga, andHartmannella vermiformis.Naegleria fowleri,Balamuthia mandrillaris, orSappiniasp. was not isolated during the study. AllA. castellaniiandA. polyphagasequence types were found to be genotype T4 that contains most of the pathogenicAcanthamoebastrains. The results indicated the occurrence and distribution of FLA species in tap water in these localities of Sivas, Turkey. Furthermore, the presence of temperature tolerantAcanthamoebagenotype T4 in tap water in the region must be taken into account for health risks.

Isolation and identification of Acanthamoeba spp. from thermal swimming pools and spas in Southern Brazil

2016 ◽  
Vol 61 (2) ◽  
Author(s):  
Laura Fuhrich Fabres ◽  
Sayonara Peixoto Rosa dos Santos ◽  
Lisianne Brittes Benitez ◽  
Marilise Brittes Rott
Keyword(s):  
Human Disease ◽  
Swimming Pools ◽  
Southern Brazil ◽  
Isolation And Identification ◽  
Free Living ◽  
Soil And Water

AbstractFree-living amoebae (FLA) are widely distributed in soil and water. A few number of them are implicated in human disease:

Isolation and identification of bovid enteroviruses from free-living wild animals in Botswana

1985 ◽  
Vol 116 (9) ◽  
pp. 238-239 ◽  
Author(s):  
C. Hamblin ◽  
N. Knowles ◽  
R. Hedger
Keyword(s):  
Wild Animals ◽  
Isolation And Identification ◽  
Free Living
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