scholarly journals Isolation and Identification of Free-Living Amoebae from Tap Water in Sivas, Turkey

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Kübra Açıkalın Coşkun ◽  
Semra Özçelik ◽  
Lütfi Tutar ◽  
Nazif Elaldı ◽  
Yusuf Tutar
Keyword(s):  
18S Rdna ◽  
Morphological Analysis ◽  
Tap Water ◽  
Acanthamoeba Castellanii ◽  
Isolation And Identification ◽  
Free Living ◽  
Rdna Gene ◽  
Hartmannella Vermiformis ◽  
Sequence Types ◽  
18S Rdna Gene

The present work focuses on a local survey of free-living amoebae (FLA) that cause opportunistic and nonopportunistic infections in humans. Determining the prevalence of FLA in water sources can shine a light on the need to prevent FLA related illnesses. A total of 150 samples of tap water were collected from six districts of Sivas province. The samples were filtered and seeded on nonnutrient agar containingEscherichia colispread. Thirty-three (22%) out of 150 samples were found to be positive for FLA. The FLA were identified by morphology and by PCR using 18S rDNA gene. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of three different species,Acanthamoeba castellanii,Acanthamoeba polyphaga, andHartmannella vermiformis.Naegleria fowleri,Balamuthia mandrillaris, orSappiniasp. was not isolated during the study. AllA. castellaniiandA. polyphagasequence types were found to be genotype T4 that contains most of the pathogenicAcanthamoebastrains. The results indicated the occurrence and distribution of FLA species in tap water in these localities of Sivas, Turkey. Furthermore, the presence of temperature tolerantAcanthamoebagenotype T4 in tap water in the region must be taken into account for health risks.

Hemiflagellochloris (Chlorophyceae, Chlorophyta), a new for Russian flora genus of green algae

2014 ◽  
Vol 48 ◽  
pp. 104-113
Author(s):  
A. D. Temraleeva ◽  
E. V. Mincheva ◽  
Yu. S. Bukin ◽  
M. V. Eltsov ◽  
V. A. Demkin ◽  
...  
Keyword(s):  
Green Algae ◽  
Molecular Analysis ◽  
18S Rdna ◽  
Phylogenetic Position ◽  
Rdna Gene ◽  
Algal Strain ◽  
The North ◽  
Volgograd Region ◽  
First Time ◽  
18S Rdna Gene

The genus Hemiflagellochloris Watanabe et al. was found in Russia for the first time. The algal strain was isolated from a buried solonetz in the zone of dry steppes (the north-end of Ergeni Hills, Volgograd Region). The morphology and ecology of this isolate are described. Its phylogenetic position within Chlorophyceae is determined by molecular analysis of 18S rDNA gene.

scholarly journals Myxobolus marajoensis sp. n. (Myxosporea: Myxobolidae), parasite of the freshwater catfish Rhamdia quelen from the Brazilian Amazon region

2017 ◽  
Vol 26 (4) ◽  
pp. 465-471 ◽  
Author(s):  
Jacqueline Abrunhosa ◽  
José Ledamir Sindeaux-Neto ◽  
Ândrea Kely dos Santos ◽  
Igor Hamoy ◽  
Edilson Matos
Keyword(s):  
New Species ◽  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Muscle Layer ◽  
Gene Sequences ◽  
Fishery Resource ◽  
Rdna Gene ◽  
Brazilian Amazon Region ◽  
Rhamdia Quelen ◽  
18S Rdna Gene

Abstract This study provides morphological and molecular data of a new parasite species found in the muscle layer of the intestinal tract of the South American silver catfish, Rhamdia quelen from Marajó Island region (Pará State, Brazil), an important fishery resource with recognized potential for fish farming. The morphology of these parasites was reanalyzed and phylogenetic analyses were run on their 18S rDNA gene sequences. The spores were morphologically distinct from those of other Myxobolus species described previously. The obtained partial sequence of the 18S rDNA gene sequences of the new species were compared to those of 24 other Myxobolus and Henneguya species available in GenBank. The results of morphological and molecular analyses indicated clearly the existence of a new species, Myxobolus marajoensis sp. n.

Polymorphisms in the 18S rDNA gene of Cystoisospora belli and clinical features of cystoisosporosis in HIV-infected patients

2010 ◽  
Vol 108 (3) ◽  
pp. 679-685 ◽  
Author(s):  
Deisy V. Resende ◽  
André L. Pedrosa ◽  
Dalmo Correia ◽  
Marlene Cabrine-Santos ◽  
Eliane Lages-Silva ◽  
...  
Keyword(s):  
Clinical Features ◽  
18S Rdna ◽  
Rdna Gene ◽  
18S Rdna Gene

Taenia spp.: 18S rDNA microsatellites for molecular systematic diagnosis

2005 ◽  
Vol 79 (2) ◽  
pp. 139-142 ◽  
Author(s):  
P. Foronda ◽  
J.C. Casanova ◽  
E. Martinez ◽  
B. Valladares ◽  
C. Feliu
Keyword(s):  
Canary Islands ◽  
18S Rdna ◽  
Oryctolagus Cuniculus ◽  
Wild Rabbit ◽  
Molecular Systematic ◽  
Rdna Gene ◽  
Larval Stages ◽  
First Time ◽  
18S Rdna Gene ◽  
Taenia Parva

AbstractThe 18S rDNA gene of adult worms of Taenia parva found in Genetta genetta in the Iberian Peninsula and larval stages of T. pisiformis from the wild rabbit (Oryctolagus cuniculus) in Tenerife (Canary Islands) were amplified and sequenced. The sequences of the 18S rDNA gene of T. parva (1768 bp) and T. pisiformis (1760 bp) are reported for the first time (GenBank accession nos. AJ555167–AJ555168 and AJ555169–AJ555170, respectively). In 168 alignment positions microsatellites in the 18S rDNA of both taxa were detected for the first time (TGC in T. parva and TGCT in T. pisiformis) and differences in their sequences with different repetition numbers were observed. The use of nucleotide sequences of this gene in the resolution of systematic problems in cestodes is discussed with reference to the systematic status of Taenia spp. and mainly in human taeniids such as T. solium, T. saginata, and Asian human isolates of Taenia.

A preliminary phylogenetic analysis of the Cymbellales based on 18S rDNA gene sequencing

2011 ◽  
Vol 26 (3) ◽  
pp. 305-315 ◽  
Author(s):  
Lenaïg Kermarrec ◽  
Luc Ector ◽  
Agnès Bouchez ◽  
Frédéric Rimet ◽  
Lucien Hoffmann
Keyword(s):  
Phylogenetic Analysis ◽  
18S Rdna ◽  
Gene Sequencing ◽  
Rdna Gene ◽  
18S Rdna Gene ◽  
Preliminary Phylogenetic Analysis

Capnodiaceous sooty mold phylogeny

1998 ◽  
Vol 76 (12) ◽  
pp. 2125-2130 ◽  
Author(s):  
Don R Reynolds
Keyword(s):  
18S Rdna ◽  
Monophyletic Group ◽  
Rdna Gene ◽  
Sooty Mold ◽  
18S Rdna Gene ◽  
Bitunicate Ascus

Sequences from the 18s rDNA gene of representatives of the capnodiaceous sooty mold families Antennulariellaceae, Capnodiaceae, and Metacapnodiaceae as well as 14 ascomycete taxa representing the Plectomycetes, Dothideales, Pyrenomycetes, and Pleosporales, and yeast outgroups were analyzed. Sooty mold capnodiaceous ascomycetes comprising were found to be a monophyletic group, the Capnodiales. The convergent origin of the bitunicate ascus associated with the periphysoid sterile element is validated. The major Capnodiales characters are the foliicolous habit, darkly pigmented hyphae, and a distinctive periphysoid sterile element associated with a fissitunicate type of bitunicate ascus.Key words: ascomycetes, Capnodiales, periphysoid sterile elements.

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