Isolation and establishment in in vitro culture of a Theileria annulata - infected cell line from Spain

1997 ◽  
Vol 83 (4) ◽  
pp. 394-396 ◽  
Author(s):  
J. Viseras ◽  
Pedro García-Fernández ◽  
Francisco Javier Adroher
Keyword(s):  
Infected Cell ◽  
In Vitro Culture ◽  
Cell Line ◽  
Theileria Annulata ◽  
Infected Cell Line

scholarly journals Determination of the Infectivity of Cryopreserved Theileria annu-lata Sporozoites in Tick Derived Stabilates Iran Ak-93 Strain, by In Vivo and In Vitro Methods

2019 ◽  
Author(s):  
Hossein MODIRROUSTA ◽  
Gholamreza HABIBI ◽  
Parviz SHAYAN ◽  
Asghar AFSHARI ◽  
Ali MIRJALILI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Cell Line ◽  
Challenge Test ◽  
Infected Cell Line ◽  
Rt Pcr ◽  
In Vitro Cell Culture ◽  
Microscopic Inspection

Background: The protozoan parasite Theileria annulata is the causative agent of tropical theileriosis in cattle. Vaccination is recommended by administration of attenuated schizont-infected cell lines. The expected protective immunity post-vaccination can be demonstrated by challenge test through inoculation of highly virulent infective sporozoites. The aim of this study was to produce Hyalomma anatolicum anatolicum tick infected with T. annulata (local strain) for preparation of tick-derived sporozoite stabilates for molecular characterization and infectivity test assay. Methods: A local T. annulata strain was used for experimental infection of calves. A field isolate of H. a. anatolicum was isolated, laboratory-reared and infected by blood-feeding on Theileria infected above-mentioned calves. The infectivity of calf, tick and prepared stabilate were confirmed by clinical signs of theileriosis, microscopic inspection, RT-PCR and in vitro cell culture. Results: The tick stabilate was prepared and cryopreserved in liquid nitrogen. The infectivity of the tick stabilate was verified by in vivo bioassay, in vitro cell culture infection, microscopic inspection in salivary glands and RT-PCR assay. The in vitro produced cell line in this study was characterized by T. annulata Cytochrome b gene analyzing. Conclusion: The infectivity of a new prepared tick-derived sporozoite stabilate was confirmed in susceptible calves; by microscopically, post mortem, tick microscopic and molecular assays. Moreover, naïve PBMCs were transformed and proliferated by T. annulata infected tick stabilate to immortal T. annulata schizont infected cell line. The potent infective sporozoite tick derived stabilate could be used for vaccine efficacy and challenge test as well as in vaccine development.

The protection afforded to cattle immunized with Theileria annulata infected cell line is enhanced by subunit vaccine candidate Ta SP

2020 ◽  
Vol 67 (S1) ◽  
pp. 26-34
Author(s):  
Abubakar A. Saaid ◽  
Diaeldin A. Salih ◽  
Limia M. Elhaj ◽  
Mohammed A. Abdalla ◽  
Maximilian Baumann ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Subunit Vaccine ◽  
Vaccine Candidate ◽  
Theileria Annulata ◽  
Infected Cell Line

Characterization of efferent lymph cells and their function following immunization of cattle with an allogenic Theileria annulata infected cell line

2003 ◽  
Vol 93 (1-2) ◽  
pp. 39-49 ◽  
Author(s):  
A.K. Nichani ◽  
J.D.M. Campbell ◽  
E.J. Glass ◽  
S.P. Graham ◽  
S.C. Craigmile ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Theileria Annulata ◽  
Infected Cell Line

scholarly journals Re-infection of the prion from the scrapie-infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

2016 ◽  
Vol 37 (3) ◽  
pp. 716-726 ◽  
Author(s):  
KANG XIAO ◽  
BAO-YUN ZHANG ◽  
XIAO-MEI ZHANG ◽  
JING WANG ◽  
CAO CHEN ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Infected Cell Line

Differential viral gene expression and its effect on the biological properties of the cell clones of an HIV-1-infected cell line

Virology ◽  
1990 ◽  
Vol 177 (1) ◽  
pp. 380-383 ◽  
Author(s):  
V.S. Kalyanaraman ◽  
V. Rodriguez ◽  
S. Josephs ◽  
R.C. Gallo ◽  
M.G. Sarngadharan
Keyword(s):  
Gene Expression ◽  
Infected Cell ◽  
Cell Line ◽  
Viral Gene Expression ◽  
Biological Properties ◽  
Viral Gene ◽  
Infected Cell Line ◽  
Cell Clones ◽  
Hiv 1

Genotypic alterations in cloned HTLV-1-infected cell line cells

1990 ◽  
Vol 1 (5) ◽  
pp. 397
Author(s):  
Keiji Iwatsuki ◽  
Masahiro Takigawa ◽  
Jin Fenxiang
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Infected Cell Line

In Vitro Culture Studies of Childhood Myelodysplastic Syndrome: Establishment of the Cell Line MUTZ-1

1997 ◽  
Vol 25 (3-4) ◽  
pp. 345-363 ◽  
Author(s):  
Klaus G. Steube ◽  
Suzanne M. Gignac ◽  
Zhen-Bo Hu ◽  
Dörthe Teepe ◽  
Dörthe Harms ◽  
...  
Keyword(s):  
Myelodysplastic Syndrome ◽  
In Vitro Culture ◽  
Cell Line ◽  
Culture Studies
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