Theileria annulata: Attenuation of a Schizont-Infected Cell Line by Prolonged in Vitro Culture Is Not Caused by the Preferential Growth of Particular Host Cell Types

2001 ◽  
Vol 98 (4) ◽  
pp. 188-205 ◽  
Author(s):  
P.M. Preston ◽  
L.A. Jackson ◽  
I.A. Sutherland ◽  
D.J. Brown ◽  
J. Schofield ◽  
...  
Keyword(s):  
Infected Cell ◽  
In Vitro Culture ◽  
Host Cell ◽  
Cell Line ◽  
Cell Types ◽  
Theileria Annulata ◽  
Infected Cell Line ◽  
Preferential Growth

Isolation and establishment in in vitro culture of a Theileria annulata - infected cell line from Spain

1997 ◽  
Vol 83 (4) ◽  
pp. 394-396 ◽  
Author(s):  
J. Viseras ◽  
Pedro García-Fernández ◽  
Francisco Javier Adroher
Keyword(s):  
Infected Cell ◽  
In Vitro Culture ◽  
Cell Line ◽  
Theileria Annulata ◽  
Infected Cell Line

scholarly journals Determination of the Infectivity of Cryopreserved Theileria annu-lata Sporozoites in Tick Derived Stabilates Iran Ak-93 Strain, by In Vivo and In Vitro Methods

2019 ◽  
Author(s):  
Hossein MODIRROUSTA ◽  
Gholamreza HABIBI ◽  
Parviz SHAYAN ◽  
Asghar AFSHARI ◽  
Ali MIRJALILI ◽  
...  
Keyword(s):  
Cell Culture ◽  
Infected Cell ◽  
Cell Line ◽  
Challenge Test ◽  
Infected Cell Line ◽  
Rt Pcr ◽  
In Vitro Cell Culture ◽  
Microscopic Inspection

Background: The protozoan parasite Theileria annulata is the causative agent of tropical theileriosis in cattle. Vaccination is recommended by administration of attenuated schizont-infected cell lines. The expected protective immunity post-vaccination can be demonstrated by challenge test through inoculation of highly virulent infective sporozoites. The aim of this study was to produce Hyalomma anatolicum anatolicum tick infected with T. annulata (local strain) for preparation of tick-derived sporozoite stabilates for molecular characterization and infectivity test assay. Methods: A local T. annulata strain was used for experimental infection of calves. A field isolate of H. a. anatolicum was isolated, laboratory-reared and infected by blood-feeding on Theileria infected above-mentioned calves. The infectivity of calf, tick and prepared stabilate were confirmed by clinical signs of theileriosis, microscopic inspection, RT-PCR and in vitro cell culture. Results: The tick stabilate was prepared and cryopreserved in liquid nitrogen. The infectivity of the tick stabilate was verified by in vivo bioassay, in vitro cell culture infection, microscopic inspection in salivary glands and RT-PCR assay. The in vitro produced cell line in this study was characterized by T. annulata Cytochrome b gene analyzing. Conclusion: The infectivity of a new prepared tick-derived sporozoite stabilate was confirmed in susceptible calves; by microscopically, post mortem, tick microscopic and molecular assays. Moreover, naïve PBMCs were transformed and proliferated by T. annulata infected tick stabilate to immortal T. annulata schizont infected cell line. The potent infective sporozoite tick derived stabilate could be used for vaccine efficacy and challenge test as well as in vaccine development.

The protection afforded to cattle immunized with Theileria annulata infected cell line is enhanced by subunit vaccine candidate Ta SP

2020 ◽  
Vol 67 (S1) ◽  
pp. 26-34
Author(s):  
Abubakar A. Saaid ◽  
Diaeldin A. Salih ◽  
Limia M. Elhaj ◽  
Mohammed A. Abdalla ◽  
Maximilian Baumann ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Subunit Vaccine ◽  
Vaccine Candidate ◽  
Theileria Annulata ◽  
Infected Cell Line

Characterization of efferent lymph cells and their function following immunization of cattle with an allogenic Theileria annulata infected cell line

2003 ◽  
Vol 93 (1-2) ◽  
pp. 39-49 ◽  
Author(s):  
A.K. Nichani ◽  
J.D.M. Campbell ◽  
E.J. Glass ◽  
S.P. Graham ◽  
S.C. Craigmile ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Theileria Annulata ◽  
Infected Cell Line

THE ESTABLISHMENT AND CHROMOSOME ANALYSIS OF A NEW CELL LINE OF CHINESE HAMSTER FROM SPONTANEOUS TRANSFORMATION IN VITRO

1971 ◽  
Vol 13 (1) ◽  
pp. 9-13 ◽  
Author(s):  
C. C. Lin ◽  
T. D. Chang ◽  
Virginia Niewczas-Late
Keyword(s):  
Cell Line ◽  
Chinese Hamster ◽  
Chromosome Analysis ◽  
Cell Types ◽  
Selective Advantage ◽  
Chinese Hamster Cell ◽  
Major Type ◽  
Spontaneous Transformation ◽  
Chinese Hamster Cell Line

A male Chinese hamster cell line has been established through spontaneous transformation in a skin culture. Chromosome studies at passage 13 revealed one major and one minor type of pseudodiploid cells (77.3 and 20%). At passage 42, only the major subline persisted (78%). The two sublines, especially the major one, had selective advantage over other cell types in this cell line probably because they were more nearly genetically balanced. Autoradiographic studies indicated no overall increase in late replicating chromosomal elements in the two sublines. Both cell types lacked the X chromosome and chromosome 6, but they were largely compensated for by the presence of new marker chromosomes. However, more chromosomal material was missing in the minor type than in the major type, and this may account for the lower adaptability of the former.

scholarly journals Re-infection of the prion from the scrapie-infected cell line SMB-S15 in three strains of mice, CD1, C57BL/6 and Balb/c

2016 ◽  
Vol 37 (3) ◽  
pp. 716-726 ◽  
Author(s):  
KANG XIAO ◽  
BAO-YUN ZHANG ◽  
XIAO-MEI ZHANG ◽  
JING WANG ◽  
CAO CHEN ◽  
...  
Keyword(s):  
Infected Cell ◽  
Cell Line ◽  
Infected Cell Line

Chagas Disease Chemotherapy: What Do We Know So Far?

2021 ◽  
Vol 27 ◽  
Author(s):  
Aline Araujo Zuma ◽  
Wanderley de Souza
Keyword(s):  
Host Cell ◽  
Chagas Disease ◽  
Chronic Phase ◽  
Cell Types ◽  
Neglected Tropical Disease ◽  
Cell Type ◽  
Cure Rate ◽  
New Compounds

: Chagas disease is a Neglected Tropical Disease (NTD), and although endemic in Latin America, affects around 6-7 million people infected worldwide. The treatment of Chagas disease is based on benznidazole and nifurtimox, which are the only available drugs. However, they are not effective during the chronic phase and cause several side effects. Furthermore, BZ promotes cure in 80% of the patients in the acute phase, but the cure rate drops to 20% in adults in the chronic phase of the disease. In this review, we present several studies published in the last six years, which describes the antiparasitic potential of distinct drugs, from the synthesis of new compounds aiming to target the parasite, as well as the repositioning and the combination of drugs. We highlight several compounds for having shown results that are equivalent or superior to BZ, which means that they should be further studied, either in vitro or in vivo. Furthermore, we stand out the differences in the effects of BZ on the same strain of T. cruzi, which might be related to methodological differences such as parasite and cell ratios, host cell type and the time of adding the drug. In addition, we discuss the wide variety of strains and also the cell types used as a host cell, which makes it difficult to compare the trypanocidal effect of the compounds.

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