Identification of a polyketide synthase gene ( pksP ) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence

1998 ◽  
Vol 187 (2) ◽  
pp. 79-89 ◽  
Author(s):  
K. Langfelder ◽  
Bernhard Jahn ◽  
Heike Gehringer ◽  
Axel Schmidt ◽  
Gerhard Wanner ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Polyketide Synthase ◽  
Polyketide Synthase Gene ◽  
Pigment Biosynthesis

scholarly journals Agrobacterium tumefaciens-Mediated Transformation of Aspergillus fumigatus: an Efficient Tool for Insertional Mutagenesis and Targeted Gene Disruption

2005 ◽  
Vol 71 (4) ◽  
pp. 1798-1802 ◽  
Author(s):  
Janyce A. Sugui ◽  
Yun C. Chang ◽  
K. J. Kwon-Chung
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Aspergillus Fumigatus ◽  
Gene Disruption ◽  
Insertional Mutagenesis ◽  
Polyketide Synthase ◽  
Random Mutagenesis ◽  
Single Copy ◽  
Efficient Tool ◽  
Bluish Green ◽  
Polyketide Synthase Gene

ABSTRACT Agrobacterium tumefaciens was used to transform Aspergillus fumigatus by either random or site-directed integration of transforming DNA (T-DNA). Random mutagenesis via Agrobacterium tumefaciens-mediated transformation (ATMT) was accomplished with T-DNA containing a hygromycin resistance cassette. Cocultivation of A. fumigatus conidia and Agrobacterium (1:10 ratio) for 48 h at 24°C resulted in high frequencies of transformation (>100 transformants/107 conidia). The majority of transformants harbored a randomly integrated single copy of T-DNA and were mitotically stable. We chose alb1, a polyketide synthase gene, as the target gene for homologous integration because of the clear phenotype difference between the white colonies of Δalb1 mutant strains and the bluish-green colonies of wild-type strains. ATMT with a T-DNA-containing alb1 disruption construct resulted in 66% albino transformants. Southern analysis revealed that 19 of the 20 randomly chosen albino transformants (95%) were disrupted by homologous recombination. These results suggest that ATMT is an efficient tool for transformation, random insertional mutagenesis, and gene disruption in A. fumigatus.

pks63787, a Polyketide Synthase Gene Responsible for the Biosynthesis of Benzenoids in the Medicinal Mushroom Antrodia cinnamomea

2016 ◽  
Vol 79 (6) ◽  
pp. 1485-1491 ◽  
Author(s):  
Po-Wei Yu ◽  
Ya-Chih Chang ◽  
Ruey-Fen Liou ◽  
Tzong-Huei Lee ◽  
Shean-Shong Tzean
Keyword(s):  
Polyketide Synthase ◽  
Medicinal Mushroom ◽  
Antrodia Cinnamomea ◽  
Polyketide Synthase Gene

scholarly journals Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0199110 ◽  
Author(s):  
Yi Wang ◽  
Changan Geng ◽  
Xiaolong Yuan ◽  
Mei Hua ◽  
Fenghua Tian ◽  
...  
Keyword(s):  
Polyketide Synthase ◽  
Usnic Acid ◽  
Acid Biosynthesis ◽  
Polyketide Synthase Gene

scholarly journals Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 324
Author(s):  
Yi Hua ◽  
Rui Pan ◽  
Xuelian Bai ◽  
Bin Wei ◽  
Jianwei Chen ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Polyketide Synthase ◽  
Gene Knockout ◽  
Gene Clusters ◽  
Inhibitory Effect ◽  
Genomic Feature ◽  
Biosynthetic Gene ◽  
Aromatic Polyketides ◽  
Staphyloccocus Aureus ◽  
Coastal Plant

The chemical investigation of one symbiotic strain, Aspergillus fumigatus D, from the coastal plant Edgeworthia chrysantha Lindl led to the isolation of eight compounds (1–8), which were respectively identified as rubrofusarin B (1), alternariol 9-O-methyl ether (2), fonsecinone D (3), asperpyrone A (4), asperpyrone D (5), fonsecinone B (6), fonsecinone A (7), and aurasperone A (8) by a combination of spectroscopic methods (1D NMR and ESI-MS) as well as by comparison with the literature data. An antimicrobial assay showed that these aromatic polyketides exhibited no remarkable inhibitory effect on Escherichia coli, Staphyloccocus aureus and Candida albicans. The genomic feature of strain D was analyzed, as well as its biosynthetic gene clusters, using antibiotics and Secondary Metabolite Analysis Shell 5.1.2 (antiSMASH). Plausible biosynthetic pathways for dimeric naphtho-γ-pyrones 3–8 were first proposed in this work. A non-reducing polyketide synthase (PKS) gene D8.t287 responsible for the biosynthesis of these aromatic polyketides 1–8 was identified and characterized by target gene knockout experiment and UPLC-MS analysis.

scholarly journals The Fusarium verticillioides FUM Gene Cluster Encodes a Zn(II)2Cys6 Protein That Affects FUM Gene Expression and Fumonisin Production

2007 ◽  
Vol 6 (7) ◽  
pp. 1210-1218 ◽  
Author(s):  
Daren W. Brown ◽  
Robert A. E. Butchko ◽  
Mark Busman ◽  
Robert H. Proctor
Keyword(s):  
Gene Cluster ◽  
Polyketide Synthase ◽  
Fusarium Verticillioides ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Regulatory Proteins ◽  
Wild Type ◽  
Polyketide Synthase Gene ◽  
Splice Forms ◽  
Self Protection

ABSTRACT Fumonisins are mycotoxins produced by some Fusarium species and can contaminate maize or maize products. Ingestion of fumonisins is associated with diseases, including cancer and neural tube defects, in humans and animals. In fungi, genes involved in the synthesis of mycotoxins and other secondary metabolites are often located adjacent to each other in gene clusters. Such genes can encode structural enzymes, regulatory proteins, and/or proteins that provide self-protection. The fumonisin biosynthetic gene cluster includes 16 genes, none of which appear to play a role in regulation. In this study, we identified a previously undescribed gene (FUM21) located adjacent to the fumonisin polyketide synthase gene, FUM1. The presence of a Zn(II)2Cys6 DNA-binding domain in the predicted protein suggested that FUM21 was involved in transcriptional regulation. FUM21 deletion (Δfum21) mutants produce little to no fumonisin in cracked maize cultures but some FUM1 and FUM8 transcripts in a liquid GYAM medium. Complementation of a Δfum21 mutant with a wild-type copy of the gene restored fumonisin production. Analysis of FUM21 cDNAs identified four alternative splice forms (ASFs), and microarray analysis indicated the ASFs were differentially expressed. Based on these data, we present a model for how FUM21 ASFs may regulate fumonisin biosynthesis.

Targeted disruption of the polyketide synthase gene pks15 affects virulence against insects and phagocytic survival in the fungus Beauveria bassiana

2017 ◽  
Vol 121 (8) ◽  
pp. 664-675 ◽  
Author(s):  
Wachiraporn Toopaang ◽  
Suranat Phonghanpot ◽  
Juntira Punya ◽  
Cheerapha Panyasiri ◽  
Kewarin Klamchao ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Polyketide Synthase ◽  
Targeted Disruption ◽  
Polyketide Synthase Gene ◽  
Fungus Beauveria Bassiana
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