Direct contacts between fibers from the ventrolateral thalamic nucleus and frontal cortical neurons projecting to the striatum: a light-microscopy study in the rat

1997 ◽  
Vol 197 (1) ◽  
pp. 77-87 ◽  
Author(s):  
Sophie Zin-Ka-Ieu ◽  
Michel Roger ◽  
P. Arnault
1986 ◽  
Vol 150 (3) ◽  
pp. 187-194 ◽  
Author(s):  
D. St. John Collier ◽  
James A. Pain ◽  
Derek G. D. Wight ◽  
Penny Lovat ◽  
Michael E. Bailey

1990 ◽  
Vol 38 (1) ◽  
pp. 79-85 ◽  
Author(s):  
J Lherminier ◽  
G Prensier ◽  
E Boudon-Padieu ◽  
A Caudwell

Flavescence dorée (FD), a grapevine yellows disease, is caused by a mycoplasma-like organism (MLO). A colloidal gold indirect immunolabeling technique identified MLO in salivary glands of a vector leafhopper, Euscelidius variegatus. After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins. Double fixation with aldehydes and osmium retroxide, followed by embedding in epon, was also performed. Thin or semi-thin serial sections were treated with polyclonal anti-FD-MLO rabbit antibodies, then with gold-conjugated anti-rabbit IgG. Labeling was revealed using the silver enhancement technique for light microscopy. MLO in frozen thin sections of glands were efficiently labeled. Optimal results were obtained with 4% paraformaldehyde-0.1% glutaraldehyde fixation and low-temperature embedding in LR White resin. Both scattered MLO and unusual dense forms of MLO were easily detected with the electron-dense gold probe. This method distinguished MLO from other membrane-limited bodies and provided a good tool for studying infection in large regions of FD-infected tissues by light microscopy.


2020 ◽  
Vol 51 (12) ◽  
pp. 5074-5083
Author(s):  
Martin Sserwadda ◽  
Nancy Nevejan ◽  
Ronald Ntanzi ◽  
Pieter Cornillie ◽  
Wim Van den Broeck ◽  
...  

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