scholarly journals Ontogenetic development of the gastrointestinal tract of African lungfish larvae Protopterus aethiopicus (Heckel 1851): A light microscopy study

2020 ◽  
Vol 51 (12) ◽  
pp. 5074-5083
Author(s):  
Martin Sserwadda ◽  
Nancy Nevejan ◽  
Ronald Ntanzi ◽  
Pieter Cornillie ◽  
Wim Van den Broeck ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Light Microscopy ◽  
Microscopy Study ◽  
Ontogenetic Development ◽  
African Lungfish ◽  
Protopterus Aethiopicus

scholarly journals The Anatomy of the Gastrointestinal Tract of the African Lungfish, Protopterus annectens

2010 ◽  
Vol 293 (7) ◽  
pp. 1146-1154 ◽  
Author(s):  
José M. Icardo ◽  
Wai P. Wong ◽  
Elvira Colvee ◽  
Ai M. Loong ◽  
Yuen K. Ip
Keyword(s):  
Gastrointestinal Tract ◽  
Protopterus Annectens ◽  
African Lungfish

The Kupffer cell in experimental extrahepatic cholestasis in the rat—a light microscopy, immunohistochemical and electron microscopy study

1986 ◽  
Vol 150 (3) ◽  
pp. 187-194 ◽  
Author(s):  
D. St. John Collier ◽  
James A. Pain ◽  
Derek G. D. Wight ◽  
Penny Lovat ◽  
Michael E. Bailey
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Kupffer Cell ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Extrahepatic Cholestasis

Respiration in the African Lungfish Protopterus Aethiopicus

1968 ◽  
Vol 49 (2) ◽  
pp. 437-452 ◽  
Author(s):  
CLAUDE LENFANT ◽  
KJELL JOHANSEN
Keyword(s):  
Gas Exchange ◽  
Haemoglobin Concentration ◽  
Temperature Shift ◽  
Mean Value ◽  
Gas Analysis ◽  
Air Exposure ◽  
Air Breathing ◽  
African Lungfish ◽  
High Degree ◽  
Protopterus Aethiopicus

1. Respiratory properties of blood and pattern of aerial and aquatic breathing and gas exchange have been studied in the African lungfish, Protopterus aethiopicus. 2. The mean value for haematocrit was 25%. Haemoglobin concentration was 6.2 g% and O2 capacity 6.8 vol. %. 3. The affinity of haemoglobin for O2 was high. P50 was 10 mm. Hg at PCOCO2, 6 mm. Hg and 25 °C. The Bohr effect was smaller than for the Australian lungfish, Neoceratodus, but exceeded that for the South American lungfish, Lepidosiren. The O2 affinity showed a larger temperature shift in Protopterus than Neoceratodus. 4. The CO2 combining power and the over-all buffering capacity of the blood exceeded values for the other lungfishes. 5. Both aerial and aquatic breathing showed a labile frequency. Air exposure elicited a marked increase in the rate of air breathing. 6. When resting in aerated water, air breathing accounted for about 90% of the O2 absorption. Aquatic gas exchange with gills and skin was 2.5 times more effective than pulmonary gas exchange in removing CO2. The low gas-exchange ratio for the lung diminished further in the interval between breaths. 7. Protopterus showed respiratory independence and a maintained O2 uptake until the ambient O2 and CO2 tensions were 85 and 35 mm. Hg respectively. A further reduction in O2 tension caused an abrupt fall in the oxygen uptake. 8. Gas analysis of blood samples drawn from unanaesthetized, free-swimming fishes attested to the important role of the lung in gas exchange and the high degree of functional separation in the circulation of oxygenated and deoxygenated blood.

scholarly journals Immunolabeling of grapevine flavescence dorée MLO in salivary glands of Euscelidius variegatus: a light and electron microscopy study.

1990 ◽  
Vol 38 (1) ◽  
pp. 79-85 ◽  
Author(s):  
J Lherminier ◽  
G Prensier ◽  
E Boudon-Padieu ◽  
A Caudwell
Keyword(s):  
Light Microscopy ◽  
Salivary Glands ◽  
Light And Electron Microscopy ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Good Tool ◽  
Tissue Samples ◽  
Thin Sections ◽  
Flavescence Dorée ◽  
Euscelidius Variegatus

Flavescence dorée (FD), a grapevine yellows disease, is caused by a mycoplasma-like organism (MLO). A colloidal gold indirect immunolabeling technique identified MLO in salivary glands of a vector leafhopper, Euscelidius variegatus. After aldehyde fixation, tissue samples were prepared by cryoultramicrotomy or embedding in acrylic resins. Double fixation with aldehydes and osmium retroxide, followed by embedding in epon, was also performed. Thin or semi-thin serial sections were treated with polyclonal anti-FD-MLO rabbit antibodies, then with gold-conjugated anti-rabbit IgG. Labeling was revealed using the silver enhancement technique for light microscopy. MLO in frozen thin sections of glands were efficiently labeled. Optimal results were obtained with 4% paraformaldehyde-0.1% glutaraldehyde fixation and low-temperature embedding in LR White resin. Both scattered MLO and unusual dense forms of MLO were easily detected with the electron-dense gold probe. This method distinguished MLO from other membrane-limited bodies and provided a good tool for studying infection in large regions of FD-infected tissues by light microscopy.

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