Structure and regulation of the Arabidopsis thaliana allene oxide synthase gene

Planta ◽  
1999 ◽  
Vol 208 (4) ◽  
pp. 463-471 ◽  
Author(s):  
I. Kubigsteltig ◽  
D. Laudert ◽  
E. W. Weiler
Keyword(s):  
Arabidopsis Thaliana ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Oxide Synthase

scholarly journals ALLENE OXIDE SYNTHASE and HYDROPEROXIDE LYASE, Two Non-Canonical Cytochrome P450s in Arabidopsis thaliana and Their Different Roles in Plant Defense

2019 ◽  
Vol 20 (12) ◽  
pp. 3064 ◽  
Author(s):  
Sachin Rustgi ◽  
Armin Springer ◽  
ChulHee Kang ◽  
Diter von Wettstein ◽  
Christiane Reinbothe ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Plant Defense ◽  
Metabolic Regulation ◽  
Insect Pests ◽  
Green Leaf Volatiles ◽  
Hydroperoxide Lyase ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Cytochrome P450 Enzymes ◽  
Oxide Synthase

The channeling of metabolites is an essential step of metabolic regulation in all living organisms. Multifunctional enzymes with defined domains for metabolite compartmentalization are rare, but in many cases, larger assemblies forming multimeric protein complexes operate in defined metabolic shunts. In Arabidopsis thaliana, a multimeric complex was discovered that contains a 13-lipoxygenase and allene oxide synthase (AOS) as well as allene oxide cyclase. All three plant enzymes are localized in chloroplasts, contributing to the biosynthesis of jasmonic acid (JA). JA and its derivatives act as ubiquitous plant defense regulators in responses to both biotic and abiotic stresses. AOS belongs to the superfamily of cytochrome P450 enzymes and is named CYP74A. Another CYP450 in chloroplasts, hydroperoxide lyase (HPL, CYP74B), competes with AOS for the common substrate. The products of the HPL reaction are green leaf volatiles that are involved in the deterrence of insect pests. Both enzymes represent non-canonical CYP450 family members, as they do not depend on O2 and NADPH-dependent CYP450 reductase activities. AOS and HPL activities are crucial for plants to respond to different biotic foes. In this mini-review, we aim to summarize how plants make use of the LOX2–AOS–AOC2 complex in chloroplasts to boost JA biosynthesis over volatile production and how this situation may change in plant communities during mass ingestion by insect pests.

scholarly journals Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

2020 ◽  
Author(s):  
Susana Serrazina ◽  
Helena Machado ◽  
Rita Costa ◽  
Paula Duque ◽  
Rui Malhó
Keyword(s):  
Arabidopsis Thaliana ◽  
Jasmonic Acid ◽  
Stress Responses ◽  
Phytophthora Cinnamomi ◽  
Defense Responses ◽  
Confocal Imaging ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Castanea Crenata ◽  
Oxide Synthase

ABSTRACTAllene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants.Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.One-sentence summaryHeterologous expression of the Castanea crenata allene oxide synthase gene in Arabidopsis thaliana improves the defense response to the pathogen Phytophthora cinnamomi.

scholarly journals Specific Adduction of Plant Lipid Transfer Protein by an Allene Oxide Generated by 9-Lipoxygenase and Allene Oxide Synthase

2006 ◽  
Vol 281 (51) ◽  
pp. 38981-38988 ◽  
Author(s):  
Bénédicte Bakan ◽  
Mats Hamberg ◽  
Ludivine Perrocheau ◽  
Daniel Maume ◽  
Hélène Rogniaux ◽  
...  
Keyword(s):  
Lipid Transfer Protein ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Lipid Transfer ◽  
Plant Lipid ◽  
Transfer Protein ◽  
Oxide Synthase

scholarly journals Functional Characterization of An Allene Oxide Synthase Involved in Biosynthesis of Jasmonic Acid and Its Influence on Metabolite Profiles and Ethylene Formation in Tea (Camellia sinensis) Flowers

2018 ◽  
Vol 19 (8) ◽  
pp. 2440 ◽  
Author(s):  
Qiyuan Peng ◽  
Ying Zhou ◽  
Yinyin Liao ◽  
Lanting Zeng ◽  
Xinlan Xu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Jasmonic Acid ◽  
Camellia Sinensis ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Tea Leaves ◽  
Ethylene Formation ◽  
Horticultural Crops ◽  
Metabolite Profiles ◽  
Oxide Synthase

Jasmonic acid (JA) is reportedly involved in the interaction between insects and the vegetative parts of horticultural crops; less attention has, however, been paid to its involvement in the interaction between insects and the floral parts of horticultural crops. Previously, we investigated the allene oxide synthase 2 (AOS2) gene that was found to be the only JA synthesis gene upregulated in tea (Camellia sinensis) flowers exposed to insect (Thrips hawaiiensis (Morgan)) attacks. In our present study, transient expression analysis in Nicotiana benthamiana plants confirmed that CsAOS2 functioned in JA synthesis and was located in the chloroplast membrane. In contrast to tea leaves, the metabolite profiles of tea flowers were not significantly affected by 10 h JA (2.5 mM) treatment as determined using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry, and gas chromatography-mass spectrometry. Moreover, JA treatment did not significantly influence ethylene formation in tea flowers. These results suggest that JA in tea flowers may have different functions from JA in tea leaves and other flowers.

scholarly journals Jasmonates: what ALLENE OXIDE SYNTHASE does for plants

2019 ◽  
Vol 70 (13) ◽  
pp. 3373-3378 ◽  
Author(s):  
Edward E Farmer ◽  
Alain Goossens
Keyword(s):  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Oxide Synthase

Allene oxide synthase and hydroperoxide lyase product accumulation in Artemisia species

Plant Science ◽  
2005 ◽  
Vol 169 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Meshack Afitlhile ◽  
Hirotada Fukushige ◽  
Charles McCraken ◽  
David Hildebrand
Keyword(s):  
Hydroperoxide Lyase ◽  
Allene Oxide ◽  
Allene Oxide Synthase ◽  
Artemisia Species ◽  
Oxide Synthase ◽  
Product Accumulation
Sign in / Sign up

Export Citation Format

Share Document