Mitochondrial oxygen consumption, lipid peroxidation and antioxidant enzyme systems in skeletal muscle of senile dystrophic mice

1998 ◽  
Vol 437 (1) ◽  
pp. 168-171 ◽  
Author(s):  
Veronika Faist ◽  
Jürgen Koenig ◽  
Harald Hoeger ◽  
Ibrahim Elmadfa
Keyword(s):  
Skeletal Muscle ◽  
Lipid Peroxidation ◽  
Oxygen Consumption ◽  
Antioxidant Enzyme ◽  
Mitochondrial Oxygen Consumption ◽  
Enzyme Systems ◽  
Dystrophic Mice

scholarly journals Low Levels of Lipopolysaccharide Modulate Mitochondrial Oxygen Consumption in Skeletal Muscle

Metabolism ◽  
2015 ◽  
Vol 64 (3) ◽  
pp. 416-427 ◽  
Author(s):  
Madlyn I. Frisard ◽  
Yaru Wu ◽  
Ryan P. McMillan ◽  
Kevin A. Voelker ◽  
Kristin A. Wahlberg ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Mitochondrial Oxygen Consumption ◽  
Low Levels

scholarly journals Mitochondrial oxygen consumption deficits in skeletal muscle isolated from an Alzheimer’s disease-relevant murine model

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Rosemary A Schuh ◽  
Kathryn C Jackson ◽  
Anna E Schlappal ◽  
Espen E Spangenburg ◽  
Christopher W Ward ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Skeletal Muscle ◽  
Alzheimer's Disease ◽  
Oxygen Consumption ◽  
Murine Model ◽  
Mitochondrial Oxygen Consumption

Effect of hyperthermia and acidosis on equine skeletal muscle mitochondrial oxygen consumption

2020 ◽  
pp. 1-10
Author(s):  
M.S. Davis ◽  
M.R. Fulton ◽  
A. Popken
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Oxidative Phosphorylation ◽  
Muscle Fatigue ◽  
Mitochondrial Function ◽  
Mitochondrial Respiration ◽  
Complex I ◽  
Mitochondrial Oxygen Consumption ◽  
Biochemical Basis ◽  
Complex Ii

The skeletal muscle of exercising horses develops pronounced hyperthermia and acidosis during strenuous or prolonged exercise, with very high tissue temperature and low pH associated with muscle fatigue or damage. The purpose of this study was to evaluate the individual effects of physiologically relevant hyperthermia and acidosis on equine skeletal muscle mitochondrial function, using ex vivo measurement of oxygen consumption to assess the function of different mitochondrial elements. Fresh triceps muscle biopsies from 6 healthy unfit Thoroughbred geldings were permeabilised to permit diffusion of small molecular weight substrates through the sarcolemma and analysed in a high resolution respirometer at 38, 40, 42, and 44 °C, and pH=7.1, 6.5, and 6.1. Oxygen consumption was measured under conditions of non-phosphorylating (leak) respiration and phosphorylating respiration through Complex I and Complex II. Data were analysed using a one-way repeated measures ANOVA and data are expressed as mean ± standard deviation. Leak respiration was ~3-fold higher at 44 °C compared to 38 °C regardless of electron source (Complex I: 22.88±3.05 vs 8.08±1.92 pmol O2/mg/s), P=0.002; Complex II: 79.14±23.72 vs 21.43±11.08 pmol O2/mg/s, P=0.022), resulting in a decrease in efficiency of oxidative phosphorylation. Acidosis had minimal effect on mitochondrial respiration at pH=6.5, but pH=6.1 resulted in a 50% decrease in mitochondrial oxygen consumption. These results suggest that skeletal muscle hyperthermia decreases the efficiency of oxidative phosphorylation through increased leak respiration, thus providing a specific biochemical basis for hyperthermia-induced muscle fatigue. The effect of myocellular acidosis on mitochondrial respiration was minimal under typical levels of acidosis, but atypically severe acidosis can lead to impairment of mitochondrial function.

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