Annexins from Ehrlich ascites cells inhibit the calcium-activated chloride current in Xenopus laevis oocytes

1997 ◽  
Vol 434 (3) ◽  
pp. 261-266 ◽  
Author(s):  
P. Bennekou ◽  
Berit I. Kristensen ◽  
Anders J. J�rgensen ◽  
Karen Eskesen
Keyword(s):  
Xenopus Laevis ◽  
Chloride Current ◽  
Xenopus Laevis Oocytes ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells

scholarly journals Induction of apoptosis using sphingolipids activates a chloride current in Xenopus laevis oocytes

2000 ◽  
Vol 279 (1) ◽  
pp. C158-C165 ◽  
Author(s):  
R. Souktani ◽  
A. Berdeaux ◽  
B. Ghaleh ◽  
J. F. Giudicelli ◽  
L. Guize ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Dna Cleavage ◽  
Ionic Currents ◽  
Chloride Current ◽  
Xenopus Laevis Oocytes ◽  
Cell Shrinkage ◽  
Transport Pathways ◽  
Kinase C ◽  
Induction Of Apoptosis ◽  
A Current

The purpose of this study was to investigate whether the cell shrinkage that occurs during apoptosis could be explained by a change of the activity in ion transport pathways. We tested whether sphingolipids, which are potent pro-apoptotic compounds, can activate ionic currents in Xenopus laevis oocytes. Apoptosis was characterized in our model by a decrease in cell volume, a loss of cell viability, and DNA cleavage. Oocytes were studied using voltage-clamp after injection with N, N-dimethyl-d-erythrosphingosine (DMS) or d-sphingosine (DS). DMS and DS activated a fast-activating, slowly inactivating, outwardly rectifying current, similar to I Cl-swell, a swelling-induced chloride current. Lowering the extracellular chloride dramatically reduced the current, and the channel was more selective for thiocyanate and iodide (thiocyanate > iodide) than for chloride. The current was blocked by 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) and lanthanum but not by niflumic acid. Oocytes injected with a pseudosubstrate inhibitor of protein kinase C (PKC), PKC-(19–31), exhibited the same current. DMS-activated current was abolished by preexposure with phorbol myristate acetate. Our results suggest that induction of apoptosis in X. laevis oocytes, using sphingolipids or PKC inhibitors, activates a current similar to swelling-induced chloride current previously described in oocytes.

scholarly journals A new system to evaluate characteristics of Niemann-Pick C1 Like 1-mediated cholesterol transport using Xenopus laevis oocytes

2021 ◽  
Vol 1863 (2) ◽  
pp. 183508
Author(s):  
Shunsuke Nashimoto ◽  
Saori Yagi ◽  
Naoki Takeda ◽  
Miku Nonaka ◽  
Yoh Takekuma ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Cholesterol Transport ◽  
Xenopus Laevis Oocytes ◽  
Niemann Pick ◽  
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