Hypomethylation of alphoid DNA and classical satellite DNA on chromosome 1, 9, 16 and Y in extraembryonic tissue

2000 ◽  
Vol 440 (S1) ◽  
pp. R190-R192 ◽  
Author(s):  
Boris Zagradisnik ◽  
Nadja Kokalj-Vokac
Keyword(s):  
Satellite Dna ◽  
Chromosome 1 ◽  
Extraembryonic Tissue ◽  
Alphoid Dna

Epigenetic control of mammalian centromere protein binding: does DNA methylation have a role?

1996 ◽  
Vol 109 (9) ◽  
pp. 2199-2206
Author(s):  
A.R. Mitchell ◽  
P. Jeppesen ◽  
L. Nicol ◽  
H. Morrison ◽  
D. Kipling
Keyword(s):  
Dna Sequences ◽  
Satellite Dna ◽  
Inbred Mouse ◽  
Inbred Mouse Strain ◽  
Chromosome 1 ◽  
Satellite Sequences ◽  
E Protein ◽  
Minor Satellite ◽  
Internal Block ◽  
Terminal Block

Chromosome 1 of the inbred mouse strain DBA/2 has a polymorphism associated with the minor satellite DNA at its centromere. The more terminal block of satellite DNA sequences on this chromosome acts as the centromere as shown by the binding of CREST ACA serum, anti-CENP-B and anti-CENP-E polyclonal sera. Demethylation of the minor satellite DNA sequences accomplished by growing cells in the presence of the drug 5-aza-2′-deoxycytidine results in a redistribution of the CENP-B protein. This protein now binds to an enlarged area on the more terminal block and in addition it now binds to the more internal block of minor satellite DNA sequences on chromosome 1. The binding of the CENP-E protein does not appear to be affected by demethylation of the minor satellite sequences. We present a model to explain these observations. This model may also indicate the mechanism by which the CENP-B protein recognises specific sites within the arrays of minor satellite DNA on mouse chromosomes.

scholarly journals Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245128
Author(s):  
Gislayne de Paula Bueno ◽  
Kaleb Pretto Gatto ◽  
Camilla Borges Gazolla ◽  
Peterson T. Leivas ◽  
Michelle M. Struett ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Morphological Changes ◽  
Hypervariable Region ◽  
5S Rdna ◽  
Evolutionary Model ◽  
Chromosome 1 ◽  
Telomeric Sequences ◽  
Chromosome Marker

Cycloramphus bolitoglossus (Werner, 1897) is a rare species with a low population density in the Serra do Mar region of Paraná and Santa Catarina, in southern Brazil. Currently, it has been assigned to the Near Threatened (NT) category in the Brazilian List of Endangered Animal Species. Here, we described the karyotype of this species for the first time and investigated the patterns of some repetitive DNA classes in the chromosomes using molecular cytogenetic approaches. We isolated, sequenced and mapped the 5S rDNA and the satellite DNA PcP190 of C. bolitoglossus, as well as mapped the telomeric sequences and seven microsatellites motifies [(GA)15, (CA)15, (GACA)4, (GATA)8, (CAG)10, (CGC)10, and (GAA)]10. Cycloramphus bolitoglossus has 2n = 26 chromosomes and a fundamental number (FN) equal to 52, with a highly conserved karyotype compared to other genus members. Comparative cytogenetic under the phylogenetic context of genus allowed evolutionary interpretations of the morphological changes in the homologs of pairs 1, 3, and 6 along with the evolutionary history of Cycloramphus. Two subtypes of 5S rDNA type II were isolated in C. bolitoglossus genome, and several comparative analysis suggests mixed effects of concerted and birth-and-death evolution acting in this repetitive DNA. The 5S rDNA II subtype “a” and “b” was mapped on chromosome 1. However, their different position along chromosome 1 provide an excellent chromosome marker for future studies. PcP190 satellite DNA, already reported for species of the families Hylidae, Hylodidae, Leptodactylidae, and Odontophrynidae, is scattered throughout the C. bolitoglossus genome, and even non-heterochromatic regions showed hybridization signals using the PcP190 probe. Molecular analysis suggests that PcP190 satellite DNA exhibit a high-level of homogenization of this sequence in the genome of C. bolitoglossus. The PcP190 satDNA from C. bolitoglossus represents a novel sequence group, compared to other anurans, based on its hypervariable region. Overall, the present data on repetitive DNA sequences showed pseudogenization evidence and corroborated the hypothesis of the emergence of satDNA from rDNA 5S clusters. These two arguments that reinforced the importance of the birth-and-death evolutionary model to explain 5S rDNA patterns found in anuran genomes.

scholarly journals 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

2013 ◽  
Vol 14 (2) ◽  
pp. 4135-4147 ◽  
Author(s):  
Elva Cortés-Gutiérrez ◽  
Brenda Ortíz-Hernández ◽  
Martha Dávila-Rodríguez ◽  
Ricardo Cerda-Flores ◽  
José Fernández ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Satellite Dna ◽  
Cervical Neoplasia ◽  
Chromosome 1 ◽  
Dna Breakage

Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens

1994 ◽  
Vol 89 (4) ◽  
pp. 459-466 ◽  
Author(s):  
E. M. J. Salentijn ◽  
N. N. Sandal ◽  
R. Klein-Lankhorst ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
...  
Keyword(s):  
Long Range ◽  
Satellite Dna ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Chromosome 1 ◽  
Resistance Locus ◽  
Beet Cyst Nematode
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