Long-range organization of a satellite DNA family flanking the beet cyst nematode resistance locus (Hs1) on chromosome-1 of B. patellaris and B. procumbens

1994 ◽  
Vol 89 (4) ◽  
pp. 459-466 ◽  
Author(s):  
E. M. J. Salentijn ◽  
N. N. Sandal ◽  
R. Klein-Lankhorst ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
...  
Keyword(s):  
Long Range ◽  
Satellite Dna ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Chromosome 1 ◽  
Resistance Locus ◽  
Beet Cyst Nematode

Isolation and characterization of RAPD-based markers linked to the beet cyst nematode resistance locus (Hs1 pat-1) on chromosome 1 of B. patellaris

1995 ◽  
Vol 90 (6) ◽  
pp. 885-891 ◽  
Author(s):  
E. M. J. Salentijn ◽  
M. J. B. Arens-De Reuver ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
W. J. Stiekema ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Cyst Nematode ◽  
Chromosome 1 ◽  
Resistance Locus ◽  
Beet Cyst Nematode ◽  
Isolation And Characterization

Isolation of DNA markers linked to a beet cyst nematode resistance locus in Beta patellaris and Beta procumbens

1992 ◽  
Vol 235 (2-3) ◽  
pp. 432-440 ◽  
Author(s):  
E. M. J. Salentijn ◽  
N. N. Sandal ◽  
W. Lange ◽  
Th. S. M. De Bock ◽  
F. A. Krens ◽  
...  
Keyword(s):  
Dna Markers ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Resistance Locus ◽  
Beta Procumbens ◽  
Beet Cyst Nematode ◽  
Beta Patellaris

RFLP mapping of a new cereal cyst nematode resistance locus in barley

1998 ◽  
Vol 117 (2) ◽  
pp. 185-187 ◽  
Author(s):  
A. R. Barr ◽  
K. J. Chalmers ◽  
A. Karakousis ◽  
J. M. Kretschmer ◽  
S. Manning ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Cyst Nematode ◽  
Rflp Mapping ◽  
Cereal Cyst Nematode ◽  
Resistance Locus

Mapping of the cyst nematode resistance locus Gpa2 in potato using a strategy based on comigrating AFLP markers

1997 ◽  
Vol 95 (5-6) ◽  
pp. 874-880 ◽  
Author(s):  
J. Rouppe van der Voort ◽  
P. Wolters ◽  
R. Folkertsma ◽  
R. Hutten ◽  
P. van Zandvoort ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Cyst Nematode ◽  
Aflp Markers ◽  
Resistance Locus

scholarly journals Copy Number Quantification for the Soybean Cyst Nematode Resistance Locus rhg1 in the Soybean Varieties of Taiwan

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1346
Author(s):  
Cheng-Chun Huang ◽  
Jiue-in Yang ◽  
Kuo-Lung Chou ◽  
Chen-Hsiang Lin ◽  
Hao-Xun Chang
Keyword(s):  
Copy Number ◽  
Soybean Cyst Nematode ◽  
Crop Protection ◽  
Nematode Resistance ◽  
The United States ◽  
Cyst Nematode ◽  
Agricultural Product ◽  
Resistance Locus ◽  
Nucleotide Polymorphisms ◽  
Market Needs

Disease resistance is one of the most successful strategies in crop protection. For example, the implementation of PI 88788 type resistance, which contains high copy numbers of Resistance to Heterodera glycines 1 (rhg1) loci, into the commercial soybean varieties of the United States has significantly reduced the yield losses caused by soybean cyst nematode (SCN, H. glycines). Vegetable soybean, or edamame, has become a major exporting agricultural product in Taiwan with an annual revenue over $80 million USD since 2017. Several local varieties have been developed to fulfill the market needs such as the traits of flavor and sweetness. However, it remains unclear if the historical breeding programs ever incorporated rhg1 resistance into the varieties of Taiwan. This study applied the TaqMan qPCR method to measure the fluorescent signals specific to the rhg1 locus on the chromosome 18 of soybean, and the ratio of VIC and FAM signals were analyzed to predict the rhg1 copy number in the 21 soybean varieties of Taiwan. The results indicated the copy number and the single nucleotide polymorphisms of the 21 soybean varieties were identical to the susceptible soybean variety ‘Williams 82’. As importation of soybean will be continuously needed to fulfill the market and because SCN is absent in the soybean fields of Taiwan, lacking rhg1 resistance in the local soybean varieties may put the edamame industry at risk and early implementation of SCN resistance in the breeding program, alongside the application of quarantine regulations, will be the key to maintain the SCN-free status and to sustain the edamame industry in Taiwan.

scholarly journals BvcZR3 and BvHs1pro-1 Genes Pyramiding Enhanced Beet Cyst Nematode (Heterodera schachtii Schm.) Resistance in Oilseed Rape (Brassica napus L.)

2019 ◽  
Vol 20 (7) ◽  
pp. 1740 ◽  
Author(s):  
Xuanbo Zhong ◽  
Qizheng Zhou ◽  
Nan Cui ◽  
Daguang Cai ◽  
Guixiang Tang
Keyword(s):  
Transgenic Plants ◽  
Sugar Beet ◽  
Oilseed Rape ◽  
Gene Pyramiding ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Heterodera Schachtii ◽  
Translocation Line ◽  
Wild Type ◽  
Beet Cyst Nematode

Beet cyst nematode (Heterodera schachtii Schm.) is one of the most damaging pests in sugar beet growing areas around the world. The Hs1pro-1 and cZR3 genes confer resistance to the beet cyst nematode, and both were cloned from sugar beet translocation line (A906001). The translocation line carried the locus from B. procumbens chromosome 1 including Hs1pro-1 gene and resistance gene analogs (RGA), which confer resistance to Heterodera schachtii. In this research, BvHs1pro-1 and BvcZR3 genes were transferred into oilseed rape to obtain different transgenic lines by A. tumefaciens mediated transformation method. The cZR3Hs1pro-1 gene was pyramided into the same plants by crossing homozygous cZR3 and Hs1pro-1 plants to identify the function and interaction of cZR3 and Hs1pro-1 genes. In vitro and in vivo cyst nematode resistance tests showed that cZR3 and Hs1pro-1 plants could be infested by beet cyst nematode (BCN) juveniles, however a large fraction of penetrated nematode juveniles was not able to develop normally and stagnated in roots of transgenic plants, consequently resulting in a significant reduction in the number of developed nematode females. A higher efficiency in inhibition of nematode females was observed in plants expressing pyramiding genes than in those only expressing a single gene. Molecular analysis demonstrated that BvHs1pro-1 and BvcZR3 gene expressions in oilseed rape constitutively activated transcription of plant-defense related genes such as NPR1 (non-expresser of PR1), SGT1b (enhanced disease resistance 1) and RAR1 (suppressor of the G2 allele of skp1). Transcript of NPR1 gene in transgenic cZR3 and Hs1pro-1 plants were slightly up-regulated, while its expression was considerably enhanced in cZR3Hs1pro-1 gene pyramiding plants. The expression of EDS1 gene did not change significantly among transgenic cZR3, Hs1pro-1 and cZR3Hs1pro-1 gene pyramiding plants and wild type. The expression of SGT1b gene was slightly up-regulated in transgenic cZR3 and Hs1pro-1 plants compared with the wild type, however, its expression was not changed in cZR3Hs1pro-1 gene pyramiding plant and had no interaction effect. RAR1 gene expression was significantly up-regulated in transgenic cZR3 and cZR3Hs1pro-1 genes pyramiding plants, but almost no expression was found in Hs1pro-1 transgenic plants. These results show that nematode resistance genes from sugar beet were functional in oilseed rape and conferred BCN resistance by activation of a CC-NBS-LRR R gene mediated resistance response. The gene pyramiding had enhanced resistance, thus offering a novel approach for the BCN control by preventing the propagation of BCN in oilseed rape. The transgenic oilseed rape could be used as a trap crop to offer an alternative method for beet cyst nematode control.

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