Genomic localization, organization and amplification of the human zinc transporter protein gene, ZNT4 , and exclusion as a candidate gene in different clinical variants of acrodermatitis enteropathica

2001 ◽  
Vol 293 (8) ◽  
pp. 392-396 ◽  
Author(s):  
Oliver Bleck ◽  
Gabrielle H. S. Ashton ◽  
Rajeev Mallipeddi ◽  
Andrew P. South ◽  
Neil V. Whittock ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Protein Gene ◽  
Zinc Transporter ◽  
Acrodermatitis Enteropathica ◽  
Transporter Protein ◽  
Clinical Variants

scholarly journals Molecular cloning and functional characterization of novel zinc transporter rZip10 (Slc39a10) involved in zinc uptake across rat renal brush-border membrane

2007 ◽  
Vol 292 (1) ◽  
pp. F217-F229 ◽  
Author(s):  
P. Kaler ◽  
R. Prasad
Keyword(s):  
Functional Characterization ◽  
Zinc Transporter ◽  
Zinc Uptake ◽  
Local Alignment ◽  
Cooh Group ◽  
Transporter Protein ◽  
Renal Brush Border Membrane ◽  
Insertional Inactivation ◽  
Zinc Concentrations ◽  
Uptake Activity

Previously, in our laboratory a 40-kDa zinc transporter protein was purified and functionally reconstituted in proteoliposomes (Kumar R, Prasad R. Biochim Biophys Acta 1419: 23–32, 1999). Furthermore, we now report the identification of Slc39a10 cDNA encoding the 40-kDa zinc transporter protein by isolating a cloned DNA complementary to zinc transporter mRNA. cDNA was constructed from immunoenriched mRNA encoding the zinc transporter. cDNA was inserted into pBR322 using poly(dC)- poly(dG) tailing. Escherichia coli DH5α cells were transformed, and colonies were screened for zinc transporter cDNA by insertional inactivation. Plasmid DNA was purified from the ampicillin-sensitive clones, and the cDNA was sequenced from both strands. A basic local alignment research tool (BLAST) search of cDNA revealed that it belongs to the Slc39 gene family of zinc transporters and was designated as Slc39a10. Zinc transporter protein deduced on the basis of cDNA sequence was named rZip10 and consists of 385 amino acids with 9 predicted transmembrane domains. The Slc39a10 gene was abundantly expressed in both rat and human tissues. Increased extracellular zinc concentration resulted in upregulation of Slc39a10 in LLC-PK1cells expressing rZip10, which was downregulated at higher zinc concentrations. These cells accumulated more zinc than control cells. rZip10-mediated zinc uptake activity was time-, temperature-, and concentration-dependent and saturable which followed Michaelis-Menten kinetics with a Kmof 19.2 μM and Vmaxof 50 pmol·min−1·mg protein−1. This activity was competitively inhibited by cadmium with Kiof 91 μM. rZip10-mediated zinc uptake was inhibited by COOH group-modifying agents such as DCC. Immunofluorescence studies showed that rZip10 localizes to the plasma membrane of LLC-PK1cells.

Anti-Zinc Transporter Protein 8 Antibody Testing Is Not Informative in Routine Prediabetes Screening in Young Patients with Autoimmune Thyroiditis and Celiac Disease

2016 ◽  
Vol 86 (2) ◽  
pp. 100-105 ◽  
Author(s):  
Daniela Larizza ◽  
Mara De Amici ◽  
Catherine Klersy ◽  
Michela Albanesi ◽  
Riccardo Albertini ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Autoimmune Thyroiditis ◽  
Young Patients ◽  
Zinc Transporter ◽  
Antibody Testing ◽  
Transporter Protein

The Dopamine Transporter Protein Gene (SLC6A3): Primary Linkage Mapping and Linkage Studies in Tourette Syndrome

Genomics ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 459-463 ◽  
Author(s):  
Joel Gelernter ◽  
David Vandenbergh ◽  
Susan D. Kruger ◽  
David L. Pauls ◽  
Roger Kurlan ◽  
...  
Keyword(s):  
Dopamine Transporter ◽  
Tourette Syndrome ◽  
Linkage Mapping ◽  
Protein Gene ◽  
Linkage Studies ◽  
Transporter Protein

Oculocutaneous albinism type 4: six novel mutations in the membrane-associated transporter protein gene and their phenotypes

2006 ◽  
Vol 19 (5) ◽  
pp. 451-453 ◽  
Author(s):  
Katsuhiko Inagaki ◽  
Tamio Suzuki ◽  
Shiro Ito ◽  
Noriyuki Suzuki ◽  
Koji Adachi ◽  
...  
Keyword(s):  
Protein Gene ◽  
Oculocutaneous Albinism ◽  
Novel Mutations ◽  
Transporter Protein

scholarly journals Clinical variants of acrodermatitis enteropathica and its co-relation with genetics

2016 ◽  
Vol 17 (1) ◽  
pp. 35
Author(s):  
Sarabjit Kaur ◽  
Ankita Sangwan ◽  
Priyadarshini Sahu ◽  
Surabhi Dayal ◽  
VijayKumar Jain
Keyword(s):  
Acrodermatitis Enteropathica ◽  
Clinical Variants
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