Bone allograft contamination in multiorgan and tissue donors

1998 ◽  
Vol 118 (3) ◽  
pp. 156-158 ◽  
Author(s):  
J. M. Segur ◽  
S. Suso ◽  
S. García ◽  
A. Combalía ◽  
R. Ramón
Keyword(s):  
Bone Allograft ◽  
Tissue Donors ◽  
Allograft Contamination

Risk assessment of arterial allograft contamination from tissue donors colonized by Candida auris

2021 ◽  
Vol 112 ◽  
pp. 49-53 ◽  
Author(s):  
V. Mirabet ◽  
C. Salvador ◽  
A. Valentín ◽  
C. Escobedo-Lucea ◽  
L. Navarro ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Candida Auris ◽  
Tissue Donors ◽  
Arterial Allograft ◽  
Allograft Contamination

scholarly journals Bacterial infections associated with allogenic bone transplantation

2015 ◽  
Vol 72 (5) ◽  
pp. 427-430 ◽  
Author(s):  
Zeljko Stepanovic ◽  
Branko Ristic
Keyword(s):  
Infection Rate ◽  
Bone Allograft ◽  
Bone Transplantation ◽  
Contamination Rate ◽  
Coagulase Negative Staphylococcus ◽  
Allogenic Bone ◽  
Bone Allografts ◽  
Allogenic Bone Transplantation ◽  
Femoral Heads ◽  
Allograft Contamination

Background/Aim. Bone allografts are frequently used in orthopedic reconstructive procedures carrying a high risk for recipients. To assess the nature and frequency of allograft contamination and associated surgical infection the case records from our institutional bone bank were reviewed. Methods. We retrospectively analyzed the microbiology of discarded bone allografts and the surgical site of the recipients. A case series of patients who acquired surgical site infection after allogenic bone transplantation was presented. Swab culturing was conducted on 309 femoral heads from living donors who underwent partial and total hip arthroplasty between January 2007 and December 2013. To prevent potential bone allograft contamination we used saline solution of 2.0 mg/ml of amikacin during thawing. The overall infection rate was analyzed in 197 recipients. Results. Of the 309 donated femoral heads, 37 were discarded due to bacterial contamination, giving the overall contamination rate of 11.97%. The postoperative survey of 213 bone allotransplantations among 197 recipients showed the infection rate of 2.03%. The coagulase-negative Staphylococcus was the most commonly identified contaminant of bone allografts and recipient surgical sites. Conclusion. The allograft contamination rate and the infection rate among recipients in our institution are in accordance with the international standards. The coagulase-negative Staphylococcus was the most commonly identified contaminant of bone allografts and recipient surgical sites. There is no strong evidence that surgical site infections were associated with bone allograft utilization. We plan further improvements in allograft handling and decontamination with highly concentrated antibiotic solutions in order to reduce infection risk for recipients.

scholarly journals Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

2020 ◽  
Vol 6 (3) ◽  
pp. 176-179
Author(s):  
Christoph Drobek ◽  
Janine Waletzko ◽  
Michael Dau ◽  
Bernhard Frerich ◽  
Volker Weißmann ◽  
...  
Keyword(s):  
High Pressure ◽  
Structural Properties ◽  
Dna Content ◽  
Bone Allograft ◽  
Initial Study ◽  
Microbiological Contamination ◽  
Test Setup ◽  
Allograft Tissue ◽  
Bone Allografts ◽  
Content Determination

AbstractHydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

scholarly journals Volumetric changes in edentulous alveolar ridge sites utilizing guided bone regeneration and a custom titanium ridge augmentation matrix (CTRAM): a case series study

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Craig E. Hofferber ◽  
J. Cameron Beck ◽  
Peter C. Liacouras ◽  
Jeffrey R. Wessel ◽  
Thu P. Getka
Keyword(s):  
Case Series ◽  
Bone Allograft ◽  
Volumetric Analysis ◽  
Collagen Membrane ◽  
Ridge Augmentation ◽  
Implant Placement ◽  
Case Series Study ◽  
Freeze Dried ◽  
Clinical Measurements ◽  
Bone Gain

Abstract Background The purpose of this study was to evaluate the volumetric changes in partially edentulous alveolar ridges augmented with customized titanium ridge augmentation matrices (CTRAM), freeze-dried bone allograft, and a resorbable collagen membrane. Methods A pre-surgical cone beam computed tomography (CBCT) scan was obtained for CTRAM design/fabrication and to evaluate pre-surgical ridge dimensions. Ridge augmentation surgery using CTRAM, freeze-dried bone allograft, and a resorbable collagen membrane was performed at each deficient site. Clinical measurements of the area of augmentation were made at the time of CTRAM placement and re-entry, and a 2nd CBCT scan 7 months after graft placement was used for volumetric analysis. Locations of each CTRAM in situ were also compared to their planned positions. Re-entry surgery and implant placement was performed 8 months after CTRAM placement. Results Nine subjects were treated with CTRAM and freeze-dried bone allograft. Four out of the nine patients enrolled (44.4%) experienced premature CTRAM exposure during healing, and in two of these cases, CTRAM were removed early. Early exposure did not result in total graft failure in any case. Mean volumetric bone gain was 85.5 ± 30.9% of planned augmentation volume (61.3 ± 33.6% in subjects with premature CTRAM exposure vs. 104.9% for subjects without premature exposure, p = 0.03). Mean horizontal augmentation (measured clinically) was 3.02 mm, and vertical augmentation 2.86 mm. Mean surgical positional deviation of CTRAM from the planned location was 1.09 mm. Conclusion The use of CTRAM in conjunction with bone graft and a collagen membrane resulted in vertical and horizontal bone gain suitable for implant placement.

scholarly journals Algorithms for the Testing of Tissue Donors for Human Immunodeficiency Virus, Hepatitis B Virus, and Hepatitis C Virus

2020 ◽  
pp. 1-10
Author(s):  
Axel Pruß ◽  
Akila Chandrasekar ◽  
Jacinto Sánchez-Ibáñez ◽  
Sophie Lucas-Samuel ◽  
Ulrich Kalus ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Tissue Donors ◽  
Occult Hbv Infection ◽  
Occult Hbv ◽  
B Virus ◽  
Immunodeficiency Virus

<b><i>Background:</i></b> Although transmission of pathogenic viruses through human tissue grafts is rare, it is still one of the most serious dreaded risks of transplantation. Therefore, in addition to the detailed medical and social history, a comprehensive serologic and molecular screening of the tissue donors for relevant viral markers for human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) is necessary. In the case of reactive results in particular, clear decisions regarding follow-up testing and the criteria for tissue release must be made. <b><i>Methods:</i></b> Based on the clinical relevance of the specific virus markers, the sensitivity of the serological and molecular biological methods used and the application of inactivation methods, algorithms for tissue release are suggested. <b><i>Results:</i></b> Compliance with the preanalytical requirements and assessment of a possible hemodilution are mandatory requirements before testing the blood samples. While HIV testing follows defined algorithms, the procedures for HBV and HCV diagnostics are under discussion. Screening and decisions for HBV are often not as simple, e.g., due to cases of occult HBV infection, false-positive anti-HBc results, or early window period positive HBV NAT results. In the case of HCV diagnostics, modern therapies with direct-acting antivirals, which are often associated with successful treatment of the infection, should be included in the decision. <b><i>Conclusion:</i></b> In HBV and HCV testing, a high-sensitivity virus genome test should play a central role in diagnostics, especially in the case of equivocal serology, and it should be the basis for the decision to release the tissue. The proposed test algorithms and decisions are also based on current European recommendations and standards for safety and quality assurance in tissue and cell banking.

scholarly journals Forearm reconstruction with bone allograft following tumor excision: A series of 10 patients with a mean follow-up of 10 years

2011 ◽  
Vol 97 (8) ◽  
pp. 793-799 ◽  
Author(s):  
T. van Isacker ◽  
O. Barbier ◽  
A. Traore ◽  
O. Cornu ◽  
F. Mazzeo ◽  
...  
Keyword(s):  
Bone Allograft ◽  
Tumor Excision

Assessment of brain death in cadaveric tissue donors

2002 ◽  
Vol 34 (6) ◽  
pp. 2344
Author(s):  
T Bredehorn ◽  
C Langer
Keyword(s):  
Brain Death ◽  
Tissue Donors
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