scholarly journals Bacterial infections associated with allogenic bone transplantation

2015 ◽  
Vol 72 (5) ◽  
pp. 427-430 ◽  
Author(s):  
Zeljko Stepanovic ◽  
Branko Ristic
Keyword(s):  
Infection Rate ◽  
Bone Allograft ◽  
Bone Transplantation ◽  
Contamination Rate ◽  
Coagulase Negative Staphylococcus ◽  
Allogenic Bone ◽  
Bone Allografts ◽  
Allogenic Bone Transplantation ◽  
Femoral Heads ◽  
Allograft Contamination

Background/Aim. Bone allografts are frequently used in orthopedic reconstructive procedures carrying a high risk for recipients. To assess the nature and frequency of allograft contamination and associated surgical infection the case records from our institutional bone bank were reviewed. Methods. We retrospectively analyzed the microbiology of discarded bone allografts and the surgical site of the recipients. A case series of patients who acquired surgical site infection after allogenic bone transplantation was presented. Swab culturing was conducted on 309 femoral heads from living donors who underwent partial and total hip arthroplasty between January 2007 and December 2013. To prevent potential bone allograft contamination we used saline solution of 2.0 mg/ml of amikacin during thawing. The overall infection rate was analyzed in 197 recipients. Results. Of the 309 donated femoral heads, 37 were discarded due to bacterial contamination, giving the overall contamination rate of 11.97%. The postoperative survey of 213 bone allotransplantations among 197 recipients showed the infection rate of 2.03%. The coagulase-negative Staphylococcus was the most commonly identified contaminant of bone allografts and recipient surgical sites. Conclusion. The allograft contamination rate and the infection rate among recipients in our institution are in accordance with the international standards. The coagulase-negative Staphylococcus was the most commonly identified contaminant of bone allografts and recipient surgical sites. There is no strong evidence that surgical site infections were associated with bone allograft utilization. We plan further improvements in allograft handling and decontamination with highly concentrated antibiotic solutions in order to reduce infection risk for recipients.

Skeletal reconstruction by vascularized allogenic bone transplantation: effects of statin in rats

2003 ◽  
Vol 76 (5) ◽  
pp. 869-871 ◽  
Author(s):  
Teruyasu Ohno ◽  
Mitsunori Shigetomi ◽  
Koichiro Ihara ◽  
Tsunemitsu Matsunaga ◽  
Takahiro Hashimoto ◽  
...  
Keyword(s):  
Bone Transplantation ◽  
Allogenic Bone ◽  
Allogenic Bone Transplantation ◽  
Skeletal Reconstruction

Effects of Cerivastatin on Vascularized Allogenic Bone Transplantation in Rats Treated with Cyclosporine A

2003 ◽  
Vol 72 (1) ◽  
pp. 50-56 ◽  
Author(s):  
T. Ohno ◽  
M. Shigetomi ◽  
K. Ihara ◽  
K. Ikeda ◽  
T. Tsubone ◽  
...  
Keyword(s):  
Cyclosporine A ◽  
Bone Transplantation ◽  
Allogenic Bone ◽  
Allogenic Bone Transplantation

scholarly journals Initial study on removing cellular residues from hydrostatic high-pressure treated allogeneic tissue using ultrasound

2020 ◽  
Vol 6 (3) ◽  
pp. 176-179
Author(s):  
Christoph Drobek ◽  
Janine Waletzko ◽  
Michael Dau ◽  
Bernhard Frerich ◽  
Volker Weißmann ◽  
...  
Keyword(s):  
High Pressure ◽  
Structural Properties ◽  
Dna Content ◽  
Bone Allograft ◽  
Initial Study ◽  
Microbiological Contamination ◽  
Test Setup ◽  
Allograft Tissue ◽  
Bone Allografts ◽  
Content Determination

AbstractHydrostatic high-pressure technology (HHD) devitalizes tissue quickly and gently, without negatively affecting the structural properties. HHD-treated tissues must be cleaned from devitalized cells. A partially automated, gentle, reproducible and timesaving rinsing test setup utilizing ultrasound is demonstrated in this study. The test setup is used to clean HHD-treated bone allografts of tissue residues and prevent microbiological contamination. A rinsing procedure is investigated. Residual DNA content determination is utilized to analyze cleaned bone allograft tissue for rinsing procedure evaluation.

P61 Producing a Safer Bone Allograft by Procesing Single Femoral Heads

2006 ◽  
Vol 16 (s1) ◽  
pp. 51-51
Author(s):  
P. Rooney ◽  
J. Mann ◽  
M. Eagle ◽  
P. Hogg ◽  
J. Kearney
Keyword(s):  
Bone Allograft ◽  
Femoral Heads

scholarly journals Effects of gamma radiation and post-operative cisplatin injection on the incorporation of bone allografts in rats

2021 ◽  
Vol 29 (3) ◽  
pp. 51-62
Author(s):  
O.Ye. Vyrva ◽  
Ya.O. Holovina ◽  
N.O. Ashukina ◽  
R.V. Malyk ◽  
Z.M. Danyshchuk
Keyword(s):  
Gamma Radiation ◽  
Bone Tissue ◽  
Intraperitoneal Injection ◽  
Fibrous Tissue ◽  
Bone Allograft ◽  
Relative Area ◽  
Control Groups ◽  
Distal Metaphysis ◽  
Bone Allografts ◽  
Oncological Patients

Background. The reconstruction of long bone defects that occur after resection of tumors is a problem that requires constant study. Bone allografts are often used in this scenario. Unfortunately, while they are prepared, allografts partially lose their strength and osteoinductive properties; their survivability in oncological patients is only 40% after 10 years. This is why the search for superior allograft treatment methods and the study of allograft remodeling and incorporation in oncological patients, whose state has been affected by radiation or chemotherapy, is an area of interest. Purpose – study the structure of bone tissue in the distal metaphysis of a rat’s femur after bone allograft implantation (sterilized using gamma radiation or antibiotic saturation) and post-operative intraperitoneal cisplatin injection. Materials and Methods. Experiments were performed on 20 male white rats aged 5–6 months at the beginning of the experiment and weighed 365.8 ± 6.4g. All rats underwent a surgery that created a defect in the distal metaphysis of the femur which was filled with a bone allograft treated with gamma radiation (Control-1 and Experimental-1 groups) or saturated with an antibiotic (Control-2 and Experimental-2 groups). 14 days after allograft implantation, animals from the control groups received an intraperitoneal injection of 2.0–2.4 ml of 0.9% NaCl, while animals from the experimental groups received 2.5mg/kg of cisplatin. Histological analysis and histomorphometry were completed 30 days after the surgery. Results. 30 days after the operation, the smallest relative area of bone tissue (11.79%) was observed in rats from the Experimental-1 group, with gamma radiation treated allografts and post-operative intraperitoneal cisplatin injections. A somewhat higher value was found in the Experimental-2 group (antibiotic saturation + cisplatin) – 31.64%. In the control groups, (intraperitoneal injection 0.9% NaCl), the relative area of bone tissue was 16.7% (Control-1, gamma radiation treatment) and 58.09% (Control-2, antibiotic saturation). The relative area of fibrous tissue was the largest in the Experimental-1 group – 31.55% and the smallest in the Control-2 group – 12.79%. Conclusions. Allograft remodeling occurs along with the formation of bone and fibrous tissue when allografts are used to fill defects in the distal femoral metaphysis of rats, However, the relative percentages of those tissues depend on the allograft sterilization method and the use of cytostatic agents. The largest relative percentage of bone tissue (58.09%) was obtained using an allograft saturated with antibiotics and without the administration of cisplatin. The smallest (11.79%), on the other hand, occurred in gamma radiation treated allografts with cisplatin injected intraperitoneally after the operation

scholarly journals Microbial Contamination of Three-Way Taps on Arterial Lines

1998 ◽  
Vol 26 (1) ◽  
pp. 51-55 ◽  
Author(s):  
W. M. Widdowson ◽  
L. Walker ◽  
J. H. Havill ◽  
J. W. Sleigh
Keyword(s):  
Blood Pressure ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Blood Culture ◽  
Microbial Contamination ◽  
General Intensive Care Unit ◽  
Contamination Rate ◽  
Coagulase Negative Staphylococcus ◽  
Potential Source ◽  
Measure Blood Pressure

Arterial lines with three-way taps are used to measure blood pressure and aspirate blood, and are a potential source of catheter-related sepsis. Swabs were taken daily from 118 three-way taps on 98 arterial lines in a general intensive care unit. Infusion lines were changed weekly but arterial cannulae were not changed routinely. An overall contamination rate of 24.6% was found with the predominant organism being coagulase negative staphylococcus. The three-way taps became increasingly contaminated with time but this was shown to be unrelated to the manipulation rates. Blood culture organisms in those showing contamination of the three-way taps showed no relationship to the bacteria causing the contamination.

Gamma Irradiated Bone Allografts Processed from Femoral Heads

2012 ◽  
Vol 2 (5) ◽  
pp. 119-126 ◽  
Author(s):  
Rita Singh ◽  
Durgeshwer Singh
Keyword(s):  
Bone Allografts ◽  
Femoral Heads ◽  
Gamma Irradiated

Bone allografts of femoral heads

1986 ◽  
Vol 68 (4) ◽  
pp. 534-537 ◽  
Author(s):  
W W Tomford ◽  
J E Ploetz ◽  
H J Mankin
Keyword(s):  
Bone Allografts ◽  
Femoral Heads

Reduction in Coagulase-Negative Staphylococcus Infection Rates in the NICU Using Evidence-Based Research

2011 ◽  
Vol 30 (3) ◽  
pp. 165-174 ◽  
Author(s):  
Ellen Kane ◽  
Gwen Bretz
Keyword(s):  
Infection Rate ◽  
Medication Administration ◽  
System Thinking ◽  
Coagulase Negative Staphylococcus ◽  
Infection Rates ◽  
Process Change ◽  
Staff Members ◽  
Policies And Procedures ◽  
Cdc Guidelines ◽  
Control And Prevention

ABSTRACTCoagulase-negative Staphylococcus (CoNS) bloodstream infection is the most common cause of sepsis in the NICU and can lead to significant morbidity and mortality. There is evidence that hand hygiene using an alcohol-based gel and wearing gloves during patient care, management of central and peripheral intravenous lines using the Centers for Disease Control and Prevention (CDC) guidelines, and a closed medication administration system can reduce the incidence to CoNS sepsis in the NICU. To successfully apply the evidence and decrease the CoNS infection rate, a systematic process is necessary. One approach to process change that significantly reduced the CoNS infection rate in a health care system with two Level III NICUs included using system thinking; working within a multidisciplinary team; using evidence to revise, develop, and implement policies and procedures; developing staff education programs; and monitoring and providing feedback to all staff members.

Finite Element Analysis Applied to Bone Remodelling in Revision Arthroplasty of the Femur using Morselized Allograft and Cement

1998 ◽  
Vol 8 (2) ◽  
pp. 51-61 ◽  
Author(s):  
J.P. Simon ◽  
J. Vander Sloten ◽  
R. Van Audekercke ◽  
G. Van Der Perre ◽  
R.S.M. Ling ◽  
...  
Keyword(s):  
Finite Element ◽  
Revision Arthroplasty ◽  
Bone Allograft ◽  
Equivalent Thickness ◽  
Element Analysis ◽  
Bone Allografts ◽  
Small Influence ◽  
Morselized Allograft ◽  
Optimal Material ◽  
Uncemented Total Hip Replacement

Finite element models have extensively been developed and reported for primary cemented and uncemented total hip replacement, but not for revision arthroplasty of the hip. A two-dimensional equivalent thickness model was developed of the proximal femur with bone grafts, a thin but uniform cement layer and a standard femoral component. This model represents the reconstructed femoral revision setting described by Gie et al (1). The effects of surface texture, cement-stem friction, cement creep and stem malplacement were evaluated. Within the range of coefficients of friction which was investigated in this study (0.03, 0.136 and 0.3) it was observed that the influence of the value of the coefficient upon the stresses in the bone-implant structure are less than 5%. Also static cement creep and certain stem malalignments which were simulated showed a relatively small influence upon the stress distribution within this structure. An agreement between trabecular orientations in the remodeled bone allograft and the principal stress orientations in this structure was observed in the calcar region. It was concluded that the remodelling which occurs in the bone allografts follows Wolff's law of optimal material use in the trabecular structure.

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