Regeneration of myelinated fiber after crush injury is retarded in sciatic nerves of mutant Japanese quails deficient in neurofilaments

1996 ◽  
Vol 92 (5) ◽  
pp. 467-472 ◽  
Author(s):  
Xin Mei Jiang ◽  
J. X. Zhao ◽  
A. Ohnishi ◽  
Chitoshi Itakura ◽  
Makoto Mizutani ◽  
...  
Keyword(s):  
Crush Injury ◽  
Myelinated Fiber ◽  
Japanese Quails ◽  
Sciatic Nerves

scholarly journals Inflammatory Profiling of Schwann Cells in Contact with Growing Axons Distal to Nerve Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Petr Dubový ◽  
Ilona Klusáková ◽  
Ivana Hradilová Svíženská
Keyword(s):  
Schwann Cells ◽  
Nerve Injury ◽  
Inflammatory Cytokines ◽  
Close Contact ◽  
Crush Injury ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Sciatic Nerves ◽  
Anti Inflammatory ◽  
Nerve Crush Injury

Activated Schwann cells distal to nerve injury upregulate inflammatory mediators, including cytokines. The goal of the present study was to investigate expression of proinflammatory (IL-1β, TNFα) and anti-inflammatory cytokines (IL-4, IL-10) in activated Schwann cells in relation to growing axons distal to crush injury of rat sciatic nerves. Seven days from sciatic nerve crush, transverse cryostat sections were cut 5 mm distal to lesion and incubated for double immunostaining to indicate Schwann cells (GFAP or S100b) and individual investigated cytokines or to demonstrate growing axons (GAP43). The Schwann cells of naïve sciatic nerves and those removed from sham-operated rats displayed similar weak immunoreactivity for the investigated cytokines. In contrast, increased intensity of cytokine immunofluorescence was found in Schwann cells distal to crush lesion. The cytokine-positive Schwann cells were found in close contact with growing axons detected by immunostaining for GAP43. The results of immunohistochemical analysis distal to nerve crush injury suggest that inflammatory profiling of Schwann cells including upregulation of both pro- and anti-inflammatory cytokines does not prevent growth of axons distal to nerve crush injury.

scholarly journals Deep Sequencing and Bioinformatic Analysis of Lesioned Sciatic Nerves after Crush Injury

PLoS ONE ◽  
2015 ◽  
Vol 10 (12) ◽  
pp. e0143491 ◽  
Author(s):  
Sheng Yi ◽  
Honghong Zhang ◽  
Leilei Gong ◽  
Jiancheng Wu ◽  
Guangbin Zha ◽  
...  
Keyword(s):  
Deep Sequencing ◽  
Bioinformatic Analysis ◽  
Crush Injury ◽  
Sciatic Nerves

scholarly journals TRANSIENT, FOCAL ACCUMULATION OF AXONAL MITOCHONDRIA DURING THE EARLY STAGES OF WALLERIAN DEGENERATION

1962 ◽  
Vol 12 (2) ◽  
pp. 361-383 ◽  
Author(s):  
Henry deF. Webster
Keyword(s):  
Guinea Pig ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Wallerian Degeneration ◽  
Osmium Tetroxide ◽  
Crush Injury ◽  
Electron Microscopic ◽  
Myelinated Fibers ◽  
Unmyelinated Axons ◽  
Sciatic Nerves

Wallerian degeneration was produced in guinea pig sciatic nerves by a crush injury. At intervals of 2, 12, 24, 36, 48, 72, and 96 hours after the crush, the nerves were fixed in osmium tetroxide, and blocks from the distal, degenerating segment identified topographically prior to embedding in Araldite or Epon. Phase and electron microscopic study of serial cross- and longitudinal sections reveals a striking, localized accumulation of axonal mitochondria which precedes or accompanies the swelling and fragmentation previously reported by others. These focal accumulations of mitochondria are transient and are most frequently observed in the paranodal axoplasm of large myelinated fibers 24 to 36 hours after crush injury, but are also occasionally identified in small myelinated fibers and unmyelinated axons. Migration and proliferation of axonal mitochondria are considered as possible explanations of these observations.

Up-Regulated Uridine Kinase Gene Identified by RLCS in the Ventral Horn after Crush Injury to Rat Sciatic Nerves

1999 ◽  
Vol 266 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Inhaeng Yuh ◽  
Takeshi Yaoi ◽  
Sachihiko Watanabe ◽  
Seiichiro Okajima ◽  
Yasusuke Hirasawa ◽  
...  
Keyword(s):  
Ventral Horn ◽  
Crush Injury ◽  
Kinase Gene ◽  
Uridine Kinase ◽  
Sciatic Nerves

Protective effects of the calcium-channel blocker flunarizine on crush injury of sciatic nerves in a rat model

2010 ◽  
Vol 58 (4) ◽  
pp. 530 ◽  
Author(s):  
Jin-Rong Tang ◽  
Le Wu ◽  
Ping Zhang ◽  
Long-Bin Yu ◽  
Qi Niu ◽  
...  
Keyword(s):  
Calcium Channel ◽  
Calcium Channel Blocker ◽  
Rat Model ◽  
Channel Blocker ◽  
Crush Injury ◽  
Protective Effects ◽  
Sciatic Nerves
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