Ultrastructural findings at the satellite cell-myofiber border in normal and diseased human muscle biopsy specimens

2001 ◽  
Vol 101 (5) ◽  
pp. 435-439 ◽  
Author(s):  
Sven Laule ◽  
Antje Bornemann
Keyword(s):  
Satellite Cell ◽  
Muscle Biopsy ◽  
Human Muscle ◽  
Biopsy Specimens ◽  
Human Muscle Biopsy ◽  
Ultrastructural Findings

A fixative for use in muscle histochemistry.

1983 ◽  
Vol 31 (1) ◽  
pp. 110-112 ◽  
Author(s):  
B P Croker ◽  
E H Bossen ◽  
N T Brinn ◽  
F A Hammond
Keyword(s):  
Muscle Biopsy ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Human Muscle ◽  
Nonspecific Esterase ◽  
Biopsy Specimens ◽  
Murine Spleen ◽  
Fresh Frozen ◽  
Myoadenylate Deaminase ◽  
Muscle Histochemistry ◽  
Human Muscle Biopsy

A fixative solution that preserves the activity of some relevant enzymes in muscle histochemistry is described. Portions of human muscle biopsy specimens and selected murine muscles were fresh frozen or placed in the fixative at room temperature for up to 1 month before freezing. Cryostat sections of fresh frozen and fixed frozen tissue were assayed for nicotinamide adenine dinucleotide phosphate (NADH)-tetrazolium reductase (NADH), several adenosine triphosphatases (ATPases), myoadenylate deaminase (MD), and phosphorylase. NADH, ATPase, and MD activity were preserved following fixation but phosphorylase was not preserved. Murine spleen and kidney were similarly tested for acid phosphatase (acid phos), alkaline phosphatase (alk phos), and nonspecific esterase (NSE). Alk phos activity was preserved but acid phos and NSE activity were significantly reduced following fixation. This fixative is useful in some circumstances for processing or shipping human muscle biopsy specimens and experimental tissues.

Analysis of cardiolipin in human muscle biopsy

2006 ◽  
Vol 831 (1-2) ◽  
pp. 63-71 ◽  
Author(s):  
Vladimir B. Ritov ◽  
Elizabeth V. Menshikova ◽  
David E. Kelley
Keyword(s):  
Muscle Biopsy ◽  
Human Muscle ◽  
Human Muscle Biopsy

Constituents of human muscle in isometric fatigue

1975 ◽  
Vol 38 (2) ◽  
pp. 208-211 ◽  
Author(s):  
J. Karlsson ◽  
C. F. Funderburk ◽  
B. Essen ◽  
A. R. Lind
Keyword(s):  
Muscle Biopsy ◽  
Maximal Voluntary Contraction ◽  
Voluntary Contraction ◽  
Human Muscle ◽  
Isometric Contractions ◽  
Biopsy Specimens ◽  
Muscle Changes

Three subjects performed five successive isometric contractions to fatigue; the tension in any one experiment was constant at tensions varying from 20 to 80% of the maximal voluntary contraction (MVC). The interval between contractions was held constant at 11 min. Muscle biopsy specimens were obtained at the start of the experiment, after the first, fourth, and fifth, and before the second and fifth of the successive contractions. The concentrations of ATP, CP, glycogen, and lactate were measured in each sample of muscle. Changes in ATP and glycogen were insufficient to be held accountable for the development of isometric fatigue. Changes in CP and lactate were large after fatigue at intermediate tensions, but those of CP were considered unlikely to be responsible for the fatigue. At tensions of 30–50% MVC the increase in lactate could be responsible for fatigue either directly or by indirect changes in pH; at higher and lower tensions the possibility that lactate is directly implicated in the development of fatigue seems remote.

Diagnostic strategies for the histological examination of muscle biopsy specimens for the assessment of vasculitis in rheumatoid arthritis

2000 ◽  
Vol 19 (24) ◽  
pp. 3433-3447 ◽  
Author(s):  
Aeilko H. Zwinderman ◽  
Alexandre E. Voskuyl ◽  
David D. Schelhaas ◽  
Sjoerd G. van Duinen ◽  
Johanna M. van der Bas ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Histological Examination ◽  
Muscle Biopsy ◽  
Diagnostic Strategies ◽  
Biopsy Specimens

Stapling Muscle Biopsy Specimens to Prevent Artifcats

JAMA ◽  
1988 ◽  
Vol 260 (3) ◽  
pp. 338 ◽  
Author(s):  
Irwin M. Siegel
Keyword(s):  
Muscle Biopsy ◽  
Biopsy Specimens

scholarly journals The diagnostic value of perivascular infiltrates in muscle biopsy specimens for the assessment of rheumatoid vasculitis

1998 ◽  
Vol 57 (2) ◽  
pp. 114-117 ◽  
Author(s):  
A. E Voskuyl ◽  
S. G van Duinen ◽  
A. H Zwinderman ◽  
F. C Breedveld ◽  
J. M W Hazes
Keyword(s):  
Muscle Biopsy ◽  
Diagnostic Value ◽  
Rheumatoid Vasculitis ◽  
Biopsy Specimens

PROTEOLYTIC AND AUTOLYTIC ACTIVITY OF SKELETAL MUSCLE IN TURNER'S SYNDROME

1967 ◽  
Vol 54 (4) ◽  
pp. 719-728
Author(s):  
V. Stånescu ◽  
Jeanne Biener ◽  
A. Grigorescu ◽  
Cornelia Diaconescu ◽  
C. Maximilian
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Turner's Syndrome ◽  
Acid Phosphatases ◽  
Turner’S Syndrome ◽  
Control Subjects ◽  
Biopsy Specimens ◽  
Autolytic Activity ◽  
Triton X

ABSTRACT Assays of acid autolysis, acid and alkaline cathepsins, GOT transaminase and acid phosphatases activities were performed on muscle biopsy specimens in 10 patients with Turner's syndrome and 7 control subjects. An increase in the acid autolysis and alkaline cathepsins activity and a non-significant increase in the acid cathepsins activity were found. GOT transaminase and acid phosphatases activities were similar in both groups, but the increase in acid phosphatases activity after triton X-100 was higher than in the controls.

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